PMID- 33462970
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20220202
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Print)
IS  - 1076-5174 (Linking)
VI  - 56
IP  - 2
DP  - 2021 Feb
TI  - HPLC-electrospray ionization-mass spectrometry optimization by high-performance 
      design of experiments for astrocyte glutamine measurement.
PG  - e4680
LID - 10.1002/jms.4680 [doi]
AB  - The amino acid glutamine (Gln) is a likely source of energy in the brain during 
      neuroglucopenia. Effects of glucose deficiency on astrocyte Gln homeostasis 
      remain unclear, as analytical tools of requisite sensitivity for quantification 
      of intracellular levels of this molecule are not currently available. Here, a 
      primary hypothalamic astrocyte culture model was used in conjunction with design 
      of experiments (DOE)-refined high-performance liquid chromatography-electrospray 
      ionization-mass spectrometry (LC-ESI-MS) methodology to investigate the 
      hypothesis that glucoprivation alters astrocyte Gln content in a sex-specific 
      manner. Critical mass spectrometric parameters for Gln derivative chromatographic 
      response were identified by comparing the performance of central composite 
      design, Box-Behnken design, and Optimal Design (OD)-A, -D, -I, -Distance, and 
      -Modified Distance DOE models. The outcomes showed that the OD-A-generated 
      response was superior relative to other design outcomes. Forecasted surface plot 
      critical mass spectrometric parameters were maximized by OD-A, OD-Distance, and 
      OD-Modified Distance designs. OD-A produced a high-performance method that 
      yielded experimental run and forecasted surface plot maximal responses. Optimized 
      mass spectrometric analysis of male versus female astrocyte Gln content provides 
      novel evidence that glucoprivation significantly depletes this amino acid in 
      female, but not in male, and that this sex-specific response may involve 
      differential sensitivity to estrogen receptor signaling. This technological 
      advance will facilitate efforts to ascertain how distinctive physiological and 
      pathophysiological stimuli impact astrocyte Gln metabolism in each sex.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Bheemanapally, Khaggeswar
AU  - Bheemanapally K
AD  - School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, 
      University of Louisiana Monroe, Monroe, Louisiana, 71201, USA.
FAU - Ibrahim, Mostafa M H
AU  - Ibrahim MMH
AD  - School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, 
      University of Louisiana Monroe, Monroe, Louisiana, 71201, USA.
FAU - Briski, Karen P
AU  - Briski KP
AUID- ORCID: 0000-0002-7648-1395
AD  - School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, 
      University of Louisiana Monroe, Monroe, Louisiana, 71201, USA.
LA  - eng
GR  - R01 DK109382/DK/NIDDK NIH HHS/United States
GR  - DK-109382/NH/NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0RH81L854J (Glutamine)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Female
MH  - Glutamine/*analysis
MH  - Male
MH  - Models, Statistical
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
PMC - PMC7874509
MID - NIHMS1665046
OTO - NOTNLM
OT  - Box-Behnken design
OT  - Optimal Design-A
OT  - Optimal Design-D
OT  - Optimal Design-Distance
OT  - Optimal Design-I
OT  - Optimal Design-Modified Distance
OT  - central composite design
EDAT- 2021/01/20 06:00
MHDA- 2021/10/21 06:00
PMCR- 2022/02/01
CRDT- 2021/01/19 06:04
PHST- 2020/10/02 00:00 [received]
PHST- 2020/11/01 00:00 [revised]
PHST- 2020/11/04 00:00 [accepted]
PHST- 2021/01/19 06:04 [entrez]
PHST- 2021/01/20 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2022/02/01 00:00 [pmc-release]
AID - 10.1002/jms.4680 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2021 Feb;56(2):e4680. doi: 10.1002/jms.4680.