PMID- 33463134
OWN - NLM
STAT- MEDLINE
DCOM- 20210930
LR  - 20210930
IS  - 1735-5249 (Electronic)
IS  - 1735-1502 (Linking)
VI  - 19
IP  - 6
DP  - 2020 Dec 19
TI  - The Effects of Particulate Matter on C57BL/6 Peritoneal and Alveolar Macrophages.
PG  - 647-659
LID - 10.18502/ijaai.v19i6.4934 [doi]
AB  - The presence of ambient particulate matter (PM) poses more dangers to human 
      health than that of other common air pollutants such as Carbon dioxide (Co2) and 
      ozone.  Epidemiologic studies show a direct correlation between PM and the risk 
      of respiratory and cardiovascular diseases. The immune system seems to play a 
      critical role in the process of these diseases. The main goal of this study was 
      to investigate the effect of Tehran particulate matter in two aerodynamic 
      diameters (PM2.5 and PM10) on alveolar macrophages (AM) from C57/BL6 mice. To 
      evaluate the inflammatory effects of PMs, cultured alveolar, and peritoneal 
      macrophages were treated with PM2.5 and PM10 (concentrations of 5 microg/mL and 10 
      microg/mL). Tumor necrosis factor-alpha (TNF-alpha) and IL-10 (representatives of 
      inflammatory and anti-inflammatory cytokines, respectively) were assessed in the 
      culture supernatant by ELISA. Expression of arginase and inducible nitric oxide 
      synthase (iNOS) genes was carried out by quantitative real-time PCR. Different 
      functional types of cultured alveolar macrophages (M1, M2) were also determined 
      in this study. Our results suggest that PM2.5 induces M1 inflammatory phenotype 
      in comparison with PM10. We found Also, an increase in TNF-alpha and M1-related gene 
      expression (iNOS), as well as a decrease in both IL-10 and M2 phenotype genes 
      (Arginase). Moreover, a reduction in phagocytic capacity and increased apoptosis 
      function of macrophage cells were detected. PM2.5 as a major component in 
      hydrocarbons has a considerable effect on polarizing the alveolar macrophages to 
      an inflammatory phenotype and eliciting lung inflammation in mice.
FAU - Rahmani, Hoda
AU  - Rahmani H
AD  - Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical 
      Science, Tehran, Iran. hodarahamani24@gmail.com.
FAU - Sadeghi, Somaye
AU  - Sadeghi S
AD  - Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical 
      Science, Tehran, Iran. somaieh.sadeghi@yahoo.com.
FAU - Taghipour, Niloofar
AU  - Taghipour N
AD  - Department of Tissue Engineering and Applied Cell Science, School of Advanced 
      Technologies in Medicine, Shahid Beheshti University of medical science, Tehran. 
      Iran. nillitaghipour@gmail.com.
FAU - Roshani, Mohsen
AU  - Roshani M
AD  - Tehran Air Quality Control Company (AQCC), Tehran, Iran. 
      mohsenroshani@hotmail.com.
FAU - Amani, Davar
AU  - Amani D
AD  - Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical 
      Science, Tehran, Iran. amanid@sums.ac.ir.
FAU - Ghazanfari, Tooba
AU  - Ghazanfari T
AD  - Department of Immunology, School of Medicine, Shahed University of Medical 
      Science, Tehran, Iran. Tghazanfari@yahoo.com.
FAU - Mosaffa, Nariman
AU  - Mosaffa N
AD  - Department of Immunology, Medicine Faculty, Shahid Beheshti University of Medical 
      Science, Tehran, Iran. yasamaryan@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20201219
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Air Pollutants)
RN  - 0 (Cytokines)
RN  - 0 (Particulate Matter)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Air Pollutants/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Arginase/metabolism
MH  - Cytokines/metabolism
MH  - Gene Expression/drug effects
MH  - Inflammation/drug therapy/metabolism
MH  - Interleukin-10/metabolism
MH  - Macrophages, Alveolar/*drug effects/metabolism
MH  - Macrophages, Peritoneal/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Particulate Matter/*pharmacology
MH  - Phagocytes/drug effects/metabolism
MH  - Phenotype
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Inflammation
OT  - Macrophages
OT  - Particulate matter
EDAT- 2021/01/20 06:00
MHDA- 2021/10/01 06:00
CRDT- 2021/01/19 07:08
PHST- 2020/05/02 00:00 [received]
PHST- 2020/09/28 00:00 [accepted]
PHST- 2021/01/19 07:08 [entrez]
PHST- 2021/01/20 06:00 [pubmed]
PHST- 2021/10/01 06:00 [medline]
AID - 10.18502/ijaai.v19i6.4934 [doi]
PST - epublish
SO  - Iran J Allergy Asthma Immunol. 2020 Dec 19;19(6):647-659. doi: 
      10.18502/ijaai.v19i6.4934.