PMID- 33465582
OWN - NLM
STAT- MEDLINE
DCOM- 20210212
LR  - 20210212
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1638
DP  - 2021 Feb 8
TI  - Size measurement of silica nanoparticles by Asymmetric Flow Field-Flow 
      Fractionation coupled to Multi-Angle Light Scattering: A comparison exercise 
      between two metrological institutes.
PG  - 461859
LID - S0021-9673(20)31133-X [pii]
LID - 10.1016/j.chroma.2020.461859 [doi]
AB  - In this work we present a comparison exercise between two metrological institutes 
      for size measurement of silica nanoparticles by Asymmetrical Flow Field-Flow 
      Fractionation (AF4) coupled to static light scattering. The work has been 
      performed in the frame of a French inter-laboratory comparison (ILC) exercise 
      organized by the nanoMetrology Club (CnM). The general aim of this 
      multi-technique comparison was to improve the measurement process for each 
      technique, after establishing a well-defined measurement procedure. The results 
      obtained by two national metrological institutes (NMIs), the LNE (France) and the 
      SMD (Belgium) by AF4-UV-DRI-MALS will be presented and discussed. Three different 
      samples were characterized: the reference material ERM(R)-FD304, which is a 
      suspension of colloidal silica in aqueous solution and two silica bimodal samples 
      consisting of two populations of SiO(2) nanoparticles of unknown size in aqueous 
      solution, with different populations' ratios. The procedure for the preparation 
      of the sample before the analysis, and main separation parameters have been 
      previously defined between the two institutes and will be described. The 
      principals measured parameters were the weight-average (d(ge_w)), number-average 
      (d(ge_n)) and z-average (d(ge_z)) geometric diameter; the average hydrodynamic 
      diameter (d(h)); and the diameter obtained by external calibration using 
      polystyrene latex standards (d(cal)). Results between the two NMIs were 
      comparable and coherent with the expected size values of those obtained by other 
      techniques like Scanning Mobility Particle Sizer (SMPS) and Scanning Electron 
      Microscopy (SEM) also involved in this ILC exercise. Where discrepancies are 
      observed, they leave the results compatible within their uncertainties and 
      underpin the challenges in analysing data and reporting results, making AF4 a 
      powerful tool to compare to other measurement techniques.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Alasonati, Enrica
AU  - Alasonati E
AD  - Department of Biomedical and Inorganic Chemistry, Laboratoire National de 
      Metrologie et d'Essais (LNE), 1 rue Gaston Boissier, 75015 Paris, France; LNE 
      Nanotech Institut, Laboratoire National de Metrologie et d'Essais (LNE), 29 av. 
      Roger Hennequin, 78197 Trappes, France. Electronic address: 
      enrica.alasonati@lne.fr.
FAU - Caebergs, Thierry
AU  - Caebergs T
AD  - FPS Economy, DG Quality and Safety, Metrology Division (SMD), Bvd du Roi Albert 
      II, 16 - 1000 Brussels, Belgium. Electronic address: 
      Thierry.Caebergs@economie.fgov.be.
FAU - Petry, Jasmine
AU  - Petry J
AD  - FPS Economy, DG Quality and Safety, Metrology Division (SMD), Bvd du Roi Albert 
      II, 16 - 1000 Brussels, Belgium. Electronic address: 
      Jasmine.Petry@economie.fgov.be.
FAU - Sebaihi, Noham
AU  - Sebaihi N
AD  - FPS Economy, DG Quality and Safety, Metrology Division (SMD), Bvd du Roi Albert 
      II, 16 - 1000 Brussels, Belgium. Electronic address: 
      Noham.Sebaihi@economie.fgov.be.
FAU - Fisicaro, Paola
AU  - Fisicaro P
AD  - Department of Biomedical and Inorganic Chemistry, Laboratoire National de 
      Metrologie et d'Essais (LNE), 1 rue Gaston Boissier, 75015 Paris, France; LNE 
      Nanotech Institut, Laboratoire National de Metrologie et d'Essais (LNE), 29 av. 
      Roger Hennequin, 78197 Trappes, France. Electronic address: 
      paola.fisicaro@lne.fr.
FAU - Feltin, Nicolas
AU  - Feltin N
AD  - LNE Nanotech Institut, Laboratoire National de Metrologie et d'Essais (LNE), 29 
      av. Roger Hennequin, 78197 Trappes, France. Electronic address: 
      nicolas.feltin@lne.fr.
LA  - eng
PT  - Journal Article
DEP - 20201229
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 059QF0KO0R (Water)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Academies and Institutes
MH  - Belgium
MH  - *Dynamic Light Scattering
MH  - *Fractionation, Field Flow
MH  - France
MH  - Nanoparticles/*analysis
MH  - Particle Size
MH  - Silicon Dioxide/*chemistry
MH  - Water/chemistry
OTO - NOTNLM
OT  - Asymmetrical Flow Field-Flow Fractionation
OT  - geometric diameter
OT  - hydrodynamic diameter
OT  - intercomparison
OT  - silica nanoparticles
OT  - static light scattering
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/01/20 06:00
MHDA- 2021/02/13 06:00
CRDT- 2021/01/19 20:11
PHST- 2020/10/03 00:00 [received]
PHST- 2020/12/22 00:00 [revised]
PHST- 2020/12/27 00:00 [accepted]
PHST- 2021/01/20 06:00 [pubmed]
PHST- 2021/02/13 06:00 [medline]
PHST- 2021/01/19 20:11 [entrez]
AID - S0021-9673(20)31133-X [pii]
AID - 10.1016/j.chroma.2020.461859 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Feb 8;1638:461859. doi: 10.1016/j.chroma.2020.461859. Epub 
      2020 Dec 29.