PMID- 33465584
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20210216
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1638
DP  - 2021 Feb 8
TI  - Simultaneous determination of five antiviral drug residues and stability studies 
      in honey using a two-step fraction capture coupled to liquid chromatography 
      tandem mass spectrometry.
PG  - 461890
LID - S0021-9673(21)00014-5 [pii]
LID - 10.1016/j.chroma.2021.461890 [doi]
AB  - An effective sample pretreatment method followed by liquid chromatography tandem 
      mass spectrometry (LC-MS/MS) was first developed for simultaneous determination 
      of five antiviral drug residues including ribavirin, moroxydine, amantadine, 
      rimantadine and memantine in honey. To adsorb analytes with different binding 
      properties and overcome the interference of sugars and uridine as endogenous 
      ribavirin structural analogs in honey, the target drugs were extracted with 1% 
      formic acid and then purified by a phenylboronic acid (PBA) solid phase 
      extraction cartridge using two-step fraction capture prior to LC-MS/MS analysis. 
      This method was validated by analyzing honey samples from nine floral origins 
      including miscellaneous flowers, citrus, vitex, rape, acacia, sunflower, linden, 
      buckwheat and jujube spiking at multiple levels, and the recoveries ranged from 
      82.46% to 116.34%, with relative standard deviations (RSDs) less than 14.58%. The 
      limits of detection (LODs) and limits of quantitation (LOQs) of moroxydine, 
      ribavirin, amantadine, rimantadine, and memantine were 0.1-2 microg/kg and 
      0.2-5 microg/kg, respectively. Depletion experiments of five antiviral drugs in honey 
      at different storage and process temperatures demonstrated that moroxydine can 
      potentially be used as a drug to cure sacbrood disease in honeybees.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Wang, Zhendong
AU  - Wang Z
AD  - Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, 
      Beijing 100093, China; College of life science, Yantai university, Yantai, 
      264005, China.
FAU - Wang, Xinran
AU  - Wang X
AD  - Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, 
      Beijing 100093, China.
FAU - Wang, Yuhan
AU  - Wang Y
AD  - Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, 
      Beijing 100093, China.
FAU - Wu, Cuiling
AU  - Wu C
AD  - Agilent Technologies Inc., Beijing 100102, China.
FAU - Zhou, Jinhui
AU  - Zhou J
AD  - Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, 
      Beijing 100093, China; Laboratory of Risk Assessment for Quality and Safety of 
      Bee Products, Ministry of Agriculture, Beijing 100093, China; Key Laboratory of 
      Bee Products for Quality and Safety Control, Ministry of Agriculture, Beijing 
      100093, China. Electronic address: zhoujinhui@caas.cn.
LA  - eng
PT  - Journal Article
DEP - 20210108
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Antiviral Agents)
RN  - BF4C9Z1J53 (Amantadine)
SB  - IM
MH  - Amantadine/analysis
MH  - Animals
MH  - Antiviral Agents/analysis
MH  - Bees
MH  - Chromatography, Liquid
MH  - Drug Residues/*analysis/chemistry
MH  - Honey/*analysis
MH  - Limit of Detection
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry/*methods
MH  - Temperature
OTO - NOTNLM
OT  - Antiviral drugs
OT  - Honey
OT  - LC-MS/MS
OT  - Stability
OT  - Two-step fraction capture
COIS- Declaration of Competing Interest The authors declare that there are no conflicts 
      of interest.
EDAT- 2021/01/20 06:00
MHDA- 2021/02/17 06:00
CRDT- 2021/01/19 20:11
PHST- 2020/09/28 00:00 [received]
PHST- 2021/01/04 00:00 [revised]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2021/01/20 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2021/01/19 20:11 [entrez]
AID - S0021-9673(21)00014-5 [pii]
AID - 10.1016/j.chroma.2021.461890 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Feb 8;1638:461890. doi: 10.1016/j.chroma.2021.461890. Epub 
      2021 Jan 8.