PMID- 33471224
OWN - NLM
STAT- MEDLINE
DCOM- 20211208
LR  - 20211214
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 44
IP  - 3
DP  - 2021 Jun
TI  - The Anti-inflammatory Protein TSG-6 Induced by S. aureus Regulates the Chemokine 
      Function of Endothelial Cells In Vitro by Inhibiting the 
      Chemokine-Glycosaminoglycan Interaction.
PG  - 1194-1202
LID - 10.1007/s10753-021-01414-1 [doi]
AB  - The aim of this study was to explore the effect of the anti-inflammatory protein 
      TSG-6 induced by Staphylococcus bacteria on the regulation of chemokine function 
      in endothelial cells by inhibiting the chemokine-glycosaminoglycan interaction. 
      To cultivate human umbilical vein endothelial cells and Staphylococcus aureus 
      bacteria, respectively, after the experiment is divided into the control group, 
      S. aureus bacteria-induced group, S. aureus bacteria glycosaminoglycans about 1 
      mg/L sugar group, S. aureus bacteria glycosaminoglycans about 5 mg/L sugar group, 
      and S. aureus bacteria glycosaminoglycans about 10 mg/L sugar group, E-selectin; 
      intercellular adhesion molecule-1 (ICAM-1); monocyte chemoattractant protein-1 
      (MCP-1); interleukin-8 (IL-8) expression level; chemokine CXCL9, CXCL10, and 
      CXCL11 mRNA and protein expression level; and TSG mRNA and protein expression 
      level were determined in each cell; the endothelial cell proliferation and 
      vascular endothelial cell function indicators were analyzed. The expression 
      levels of E-selectin, ICAM-1, IL-8, MCP-1, and chemokines CXCL9, CXCL10, and 
      CXCL11 mRNA and protein in each group at 6, 12, and 24 h were significantly 
      different (P < 0.05). TSG mRNA and protein expression levels, endothelial cell 
      proliferation ability, and vascular endothelial cell function were also 
      significantly different (P < 0.05). The expression levels of E-selectin, ICAM-1, 
      IL-8, MCP-1, endothelial cell proliferation ability, and vascular endothelial 
      cell function in the Staphylococcus aureus-induced group were lower than those in 
      the control group and the Staphylococcus aureus glycosaminoglycan group, and the 
      mRNA and protein expression levels of chemokines CXCL9, CXCL10, and CXCL11, and 
      TSG mRNA and protein expression levels were higher. With the increase of 
      glycosaminoglycan concentration, the above indexes were improved. The 
      anti-inflammatory protein TSG-6 induced by S. aureus can regulate the chemokine 
      function of endothelial cells by inhibiting the chemokine-glycosaminoglycan 
      interaction.
FAU - Yang, Zheng
AU  - Yang Z
AD  - Vascular Surgery, No. 2 Hospital of Baoding, Baoding, 071000, China.
FAU - Zhang, Liang
AU  - Zhang L
AUID- ORCID: 0000-0002-8105-8241
AD  - Vascular Surgery, No. 2 Hospital of Baoding, Baoding, 071000, China. 
      zhangliang8220@163.com.
FAU - Zhang, Hongguang
AU  - Zhang H
AD  - Vascular Surgery, No. 2 Hospital of Baoding, Baoding, 071000, China.
FAU - Zhou, Chenguang
AU  - Zhou C
AD  - Vascular Surgery, No. 2 Hospital of Baoding, Baoding, 071000, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20210120
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (E-Selectin)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (ICAM1 protein, human)
RN  - 0 (SELE protein, human)
RN  - 0 (TNFAIP6 protein, human)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokines/genetics/*metabolism
MH  - E-Selectin/genetics/metabolism
MH  - Glycosaminoglycans/*metabolism
MH  - Host-Pathogen Interactions
MH  - Human Umbilical Vein Endothelial Cells/*metabolism/*microbiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Protein Binding
MH  - Staphylococcus aureus/*metabolism/pathogenicity
OTO - NOTNLM
OT  - S. aureus bacteria
OT  - anti-inflammatory protein TSG-6
OT  - endothelial chemokine function
OT  - glycosaminoglycan
EDAT- 2021/01/21 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/01/20 12:23
PHST- 2020/11/15 00:00 [received]
PHST- 2021/01/02 00:00 [accepted]
PHST- 2020/12/23 00:00 [revised]
PHST- 2021/01/21 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/01/20 12:23 [entrez]
AID - 10.1007/s10753-021-01414-1 [pii]
AID - 10.1007/s10753-021-01414-1 [doi]
PST - ppublish
SO  - Inflammation. 2021 Jun;44(3):1194-1202. doi: 10.1007/s10753-021-01414-1. Epub 
      2021 Jan 20.