PMID- 33472479
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1473-2300 (Electronic)
IS  - 0300-0605 (Print)
IS  - 0300-0605 (Linking)
VI  - 49
IP  - 1
DP  - 2021 Jan
TI  - Dietary chlorogenic acid ameliorates oxidative stress and improves endothelial 
      function in diabetic mice via Nrf2 activation.
PG  - 300060520985363
LID - 10.1177/0300060520985363 [doi]
LID - 0300060520985363
AB  - OBJECTIVES: Chlorogenic acid (CGA) is an antioxidant dietary factor. We 
      investigated the effects of CGA on endothelial cell dysfunction in diabetic mice 
      and the mechanistic role of nuclear factor erythroid-related factor 2 (Nrf2) in 
      the antioxidant effect of CGA. METHODS: Diabetic (db/db) mice were fed normal 
      chow or chow containing 0.02% CGA for 12 weeks. Human umbilical vein endothelial 
      cells (HUVECs) and mouse aortas were treated with normal or high glucose. 
      RESULTS: CGA treatment induced upregulation of Nrf2 in HUVECs in a dose-dependent 
      manner. CGA pretreatment prevented reactive oxygen species generation and 
      preserved nitric oxide bioavailability in HUVECs and aortas from wild-type but 
      not Nrf2(-/-) mice. CGA improved endothelium-dependent relaxation in high 
      glucose-treated aortas from wild-type and db/db mice, but not Nrf2(-/-) mice. 
      Dietary CGA improved endothelium-dependent relaxation in db/db mice. CONCLUSIONS: 
      CGA ameliorates endothelial dysfunction in diabetic mice through activation of 
      the Nrf2 anti-oxidative pathway.
FAU - Wang, Dan
AU  - Wang D
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Hou, Jixin
AU  - Hou J
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Wan, Jindong
AU  - Wan J
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Yang, Yi
AU  - Yang Y
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Liu, Sen
AU  - Liu S
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Li, Xiaoqing
AU  - Li X
AD  - School of Pharmacy, Chengdu Medical College, Chengdu, Sichuan, China.
FAU - Li, Wenzhang
AU  - Li W
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Dai, Xiaozhen
AU  - Dai X
AD  - School of Biological Sciences and Technology, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
FAU - Liu, Weihua
AU  - Liu W
AD  - Department of Scientific Research, The First Affiliated Hospital, Chengdu Medical 
      College, Chengdu, Sichuan, China.
FAU - Wang, Peijian
AU  - Wang P
AUID- ORCID: 0000-0002-3622-4538
AD  - Department of Cardiology, The First Affiliated Hospital, Chengdu Medical College, 
      Chengdu, Sichuan, China.
AD  - Key Laboratory of Aging and Vascular Homeostasis, Sichuan Higher Education 
      Institute, The First Affiliated Hospital, Chengdu Medical College, Chengdu, 
      Sichuan, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Int Med Res
JT  - The Journal of international medical research
JID - 0346411
RN  - 0 (NF-E2-Related Factor 2)
RN  - 318ADP12RI (Chlorogenic Acid)
SB  - IM
MH  - Animals
MH  - Chlorogenic Acid/metabolism/pharmacology
MH  - *Diabetes Mellitus, Experimental/drug therapy
MH  - Endothelium, Vascular
MH  - Mice
MH  - *NF-E2-Related Factor 2/metabolism
MH  - Oxidative Stress
PMC - PMC7829538
OTO - NOTNLM
OT  - Chlorogenic acid
OT  - diabetes
OT  - endothelial dysfunction
OT  - endothelium
OT  - nuclear factor E2-related factor 2
OT  - oxidative stress
COIS- Declaration of conflicting interest: The authors declare that there is no 
      conflict of interest.
EDAT- 2021/01/22 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/01/20
CRDT- 2021/01/21 05:23
PHST- 2021/01/21 05:23 [entrez]
PHST- 2021/01/22 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/01/20 00:00 [pmc-release]
AID - 10.1177_0300060520985363 [pii]
AID - 10.1177/0300060520985363 [doi]
PST - ppublish
SO  - J Int Med Res. 2021 Jan;49(1):300060520985363. doi: 10.1177/0300060520985363.