PMID- 33472586
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20240226
IS  - 2661-8850 (Electronic)
IS  - 2661-8850 (Linking)
VI  - 22
IP  - 1
DP  - 2021 Jan 20
TI  - circRNA_0005529 facilitates growth and metastasis of gastric cancer via 
      regulating miR-527/Sp1 axis.
PG  - 6
LID - 10.1186/s12860-020-00340-8 [doi]
LID - 6
AB  - BACKGROUND: Circular RNAs (circRNAs) are endogenous non-coding RNAs, which are 
      associated with various biological processes, including microRNA (miRNA) 
      interaction, protein binding and regulatory splicing. circRNA_0005529 
      (circ_0005529) is derived from vacuolar protein sorting 33 homologue B (VPS33B), 
      and its biological role in gastric cancer (GC) has not been examined. In this 
      study, the expression and location of circ_0005529 and microRNA-527 (miR-527) 
      were determined by qRT-PCR and fluorescence in situ hybridization (FISH). Cell 
      proliferation and cell migration were determined by MTT, EdU incorporation, 
      colony formation, wound scratch and transwell assays. In addition, 
      immunohistochemistry and western blotting were performed to determine the 
      expressions of specificity protein 1 (Sp1), PCNA, c-myc, E-cadherin and 
      N-cadherin. Western blotting and luciferase reporter assay were performed to 
      study the interaction between circ_0005529 and miR-527 or miR-527 and Sp1. The 
      functional effects of circ_0005529 on GC through regulating Sp1 were further 
      evaluated using xenograft and metastatic mouse models in vivo. RESULTS: Our 
      results showed that circ_0005529 was upregulated in GC tissues and cells, and had 
      promoting effects on cell proliferation and cell migration. Mechanism analysis 
      suggested that circ_0005529 could bind to microRNA-527 (miR-527) and reduce its 
      expression. The interaction between miR-527 and Sp1 in GC was systematically 
      studied. In addition, the results indicated that Sp1 upregulation could rescue 
      the effects on cell proliferation and migration caused by circ_0005529. Moreover, 
      the inhibitory effects of circ_0005529 downregulation on GC growth and metastasis 
      were evaluated in mouse models. These findings suggested that the axis of 
      circ_0005529/miR-527/Sp1 may serve as a promising treatment target for GC 
      diagnosis and treatment. CONCLUSIONS: These findings suggested that the signal 
      axis of circ_0005529/miR-527/Sp1 may has the potential to be explored as a novel 
      therapeutic target for GC diagnosis and treatment. Mechanism diagram: During GC 
      development, overexpressed circ_0005529 sponged miR-527 and then upregulated the 
      expression of Sp1. Subsequently, epithelial-mesenchymal transition (EMT), cell 
      proliferation and cell migration were promoted, which ultimately facilitated the 
      tumor metastasis.
FAU - Zhang, Xing
AU  - Zhang X
AD  - Department of Gastrointestinal Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Yang, Hongwei
AU  - Yang H
AD  - Department of Breast and Thyroid Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Jia, Yingdong
AU  - Jia Y
AD  - Department of Gastrointestinal Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Xu, Zhengwen
AU  - Xu Z
AD  - Department of Gastrointestinal Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Zhang, Liuping
AU  - Zhang L
AD  - Department of Gastrointestinal Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Sun, Meng
AU  - Sun M
AD  - Department of Gastrointestinal Surgery, Suining Central Hospital, Suining City, 
      629000, Sichuan Province, China.
FAU - Fu, Jing
AU  - Fu J
AUID- ORCID: 0000-0001-7887-0266
AD  - Department of Emergency, Huai'an Hospital Affiliated of Xuzhou Medical University 
      and Huai'an Second People's Hospital, No. 62 Huaihai South Road, Huai 'an City, 
      Jiangsu Province, China. GHUI6trf89@163.com.
LA  - eng
GR  - 130493/Scientific Research Task of Sichuan Provincial Health Department/
PT  - Journal Article
DEP - 20210120
PL  - England
TA  - BMC Mol Cell Biol
JT  - BMC molecular and cell biology
JID - 101741148
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN527 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (Sp1 Transcription Factor)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Aged
MH  - Animals
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - RNA, Circular/genetics/*metabolism
MH  - Sp1 Transcription Factor/*genetics
MH  - Stomach Neoplasms/genetics
MH  - Up-Regulation/genetics
MH  - Mice
PMC - PMC7816457
OTO - NOTNLM
OT  - Cell migration
OT  - Cell proliferation
OT  - Gastric cancer
OT  - Sp1
OT  - circ_0005529
OT  - miR-527
COIS- The authors state that there are no conflicts of interest to disclose.
EDAT- 2021/01/22 06:00
MHDA- 2021/08/27 06:00
PMCR- 2021/01/20
CRDT- 2021/01/21 05:24
PHST- 2020/07/01 00:00 [received]
PHST- 2020/12/22 00:00 [accepted]
PHST- 2021/01/21 05:24 [entrez]
PHST- 2021/01/22 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2021/01/20 00:00 [pmc-release]
AID - 10.1186/s12860-020-00340-8 [pii]
AID - 340 [pii]
AID - 10.1186/s12860-020-00340-8 [doi]
PST - epublish
SO  - BMC Mol Cell Biol. 2021 Jan 20;22(1):6. doi: 10.1186/s12860-020-00340-8.