PMID- 33472642
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20240226
IS  - 1749-799X (Electronic)
IS  - 1749-799X (Linking)
VI  - 16
IP  - 1
DP  - 2021 Jan 20
TI  - miRNA-128 modulates bone neoplasms cells proliferation and migration through the 
      WNT/beta-catenin and EMT signal pathways.
PG  - 71
LID - 10.1186/s13018-020-02164-w [doi]
LID - 71
AB  - BACKGROUND: Bone neoplasms present poor prognosis due to recurrence and 
      metastasis. Although the role microRNAs (miRNAs) in inhibiting growth and 
      metastasis of bone neoplasms has been investigated, the underlying potential 
      molecular mechanisms mediated by miRNA-128 (miR-218) for the invasiveness of bone 
      neoplasms cells are still not completely understood. The purpose of this study 
      was to identify the regulatory mechanisms of miR-218 in bone neoplasms cells. 
      METHODS: Western blotting, quantitative reverse transcription-polymerase chain 
      reaction (qRT-PCR), Counting Kit-8 assay, terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining, luciferase activity 
      assay immunofluorescence, and immunohistochemistry were used to analyze the 
      regulatory effects of miR-218 on bone neoplasms cells. RESULTS: Here, the results 
      showed that transfection of miR-128 suppressed bone neoplasms cells 
      proliferation, migration, and invasion. Genetic knockdown of miR-128 in bone 
      neoplasms cells suppressed the activation of the Wnt/beta-catenin and 
      epithelial-mesenchymal transition (EMT) signaling pathways. Activation of Wnt or 
      EMT blocked miR-128-inhibited cells proliferation and migration in bone neoplasms 
      cells. Exogenously introduced miR-128 markedly inhibited tumor regeneration in 
      bone neoplasms xenograft models. CONCLUSIONS: These results define a 
      tumor-regulated function for miR-128 in bone neoplasms by down-regulation of the 
      Wnt/beta-catenin and EMT signal pathways, which provided a potential target for bone 
      neoplasms gene therapy.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Orthopedics, Chongqing General Hospital, University of the Chinese 
      Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 
      400013, China.
FAU - Long, Xiaotao
AU  - Long X
AD  - Department of Orthopedics, Chongqing General Hospital, University of the Chinese 
      Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 
      400013, China.
FAU - Wang, Ji
AU  - Wang J
AD  - Department of Orthopedics, Chongqing General Hospital, University of the Chinese 
      Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 
      400013, China.
FAU - Peng, Jing
AU  - Peng J
AD  - Department of Orthopedics, Chongqing General Hospital, University of the Chinese 
      Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 
      400013, China.
FAU - Shen, Kai
AU  - Shen K
AD  - Department of Orthopedics, Chongqing General Hospital, University of the Chinese 
      Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 
      400013, China. kai_shenedu@163.com.
LA  - eng
GR  - (20160131)/science and Technology Foundation of Yuzhong District in Chongqing/
PT  - Journal Article
DEP - 20210120
PL  - England
TA  - J Orthop Surg Res
JT  - Journal of orthopaedic surgery and research
JID - 101265112
RN  - 0 (MicroRNAs)
RN  - 0 (Organic Cation Transport Proteins)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
RN  - 0 (solute carrier family 22 (organic cation transporter), member 3)
SB  - IM
MH  - Animals
MH  - Bone Neoplasms/*genetics/*pathology/therapy
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/*genetics
MH  - Disease Models, Animal
MH  - Down-Regulation/genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice, Nude
MH  - MicroRNAs/*physiology/therapeutic use
MH  - Middle Aged
MH  - Molecular Targeted Therapy
MH  - Neoplasm Transplantation
MH  - Organic Cation Transport Proteins/*metabolism
MH  - Signal Transduction/*genetics/*physiology
MH  - Wnt Proteins/*metabolism
MH  - beta Catenin/*metabolism
MH  - Mice
PMC - PMC7816476
OTO - NOTNLM
OT  - Bone neoplasms
OT  - EMT
OT  - Migration
OT  - Proliferation
OT  - Wnt/beta-catenin
OT  - miRNA-128
COIS- The authors declare that they have no competing interests.
EDAT- 2021/01/22 06:00
MHDA- 2021/07/13 06:00
PMCR- 2021/01/20
CRDT- 2021/01/21 05:24
PHST- 2020/08/21 00:00 [received]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2021/01/21 05:24 [entrez]
PHST- 2021/01/22 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2021/01/20 00:00 [pmc-release]
AID - 10.1186/s13018-020-02164-w [pii]
AID - 2164 [pii]
AID - 10.1186/s13018-020-02164-w [doi]
PST - epublish
SO  - J Orthop Surg Res. 2021 Jan 20;16(1):71. doi: 10.1186/s13018-020-02164-w.