PMID- 33475929
OWN - NLM
STAT- MEDLINE
DCOM- 20210519
LR  - 20210519
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 48
IP  - 2
DP  - 2021 Feb
TI  - Influence of microcurrent on the modulation of remodelling genes in a wound 
      healing assay.
PG  - 1233-1241
LID - 10.1007/s11033-021-06135-0 [doi]
AB  - The literature has shown the beneficial effects of microcurrent (MC) therapy on 
      tissue repair. We investigated if the application of MC at 10 muA/90 s could 
      modulate the expression of remodeling genes transforming growth factor beta 
      (Tgfb), connective tissue growth factor (Ctgf), insulin-like growth factor 1 
      (Igf1), tenascin C (Tnc), Fibronectin (Fn1), Scleraxis (Scx), Fibromodulin (Fmod) 
      and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell 
      migration was analyzed between days 0 and 4 in both fibroblasts (F) and 
      fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene 
      expression were also analyzed after daily MC application. Higher expression of 
      Ctgf and lower expression of Tnc and Fmod, respectively, were observed in the 
      F+MC group in relation to F group (p < 0.05), and no difference was observed 
      between the groups for the genes Tgfb, Fn1 and Scx. In cell migration, a higher 
      number of cells in the scratch region was observed in group F+MC (p < 0.05) 
      compared to group F on the 4th day, and the cell viability assay showed no 
      difference between the groups. In conclusion, MC therapy at an intensity/time of 
      10 muA/90 s with 4 daily applications did not affect cell viability, stimulated 
      fibroblasts migration with the involvement of Ctgf, and reduced the Tnc and Fmod 
      expression.
FAU - Silva, Daniela Fernanda Dezotti
AU  - Silva DFD
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - de Oliveira Fujii, Lucas
AU  - de Oliveira Fujii L
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - Chiarotto, Gabriela Bortolanca
AU  - Chiarotto GB
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - de Oliveira, Camila Andrea
AU  - de Oliveira CA
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - de Andrade, Thiago Antonio Moretti
AU  - de Andrade TAM
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - de Oliveira, Alexandre Leite Rodrigues
AU  - de Oliveira ALR
AD  - Department of Structural and Functional Biology, Institute of Biology, State 
      University of Campinas-UNICAMP, Campinas, Sao Paulo, Brazil.
FAU - Esquisatto, Marcelo Augusto Marretto
AU  - Esquisatto MAM
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - Mendonca, Fernanda Aparecida Sampaio
AU  - Mendonca FAS
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - Dos Santos, Glaucia Maria Tech
AU  - Dos Santos GMT
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil.
FAU - de Aro, Andrea Aparecida
AU  - de Aro AA
AUID- ORCID: 0000-0003-2895-0000
AD  - Biomedical Sciences Graduate Program, University Center of Herminio Ometto 
      Foundation/FHO, Araras, Sao Paulo, Brazil. andreaaro80@gmail.com.
AD  - Department of Structural and Functional Biology, Institute of Biology, State 
      University of Campinas-UNICAMP, Campinas, Sao Paulo, Brazil. 
      andreaaro80@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210121
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (CCN2 protein, mouse)
RN  - 0 (Fibronectins)
RN  - 0 (Fmod protein, mouse)
RN  - 0 (Tenascin)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Tnc protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (insulin-like growth factor-1, mouse)
RN  - 126468-95-9 (Fibromodulin)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animals
MH  - Cell Movement/radiation effects
MH  - Connective Tissue Growth Factor/*genetics
MH  - *Electric Stimulation Therapy
MH  - Fibromodulin/*genetics
MH  - Fibronectins/genetics
MH  - Gene Expression Regulation/radiation effects
MH  - Humans
MH  - Insulin-Like Growth Factor I/genetics
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Tenascin/*genetics
MH  - Transforming Growth Factor beta1/genetics
MH  - Wound Healing/genetics/*radiation effects
OTO - NOTNLM
OT  - Cell migration
OT  - Fibroblasts
OT  - Genes
OT  - Microcurrent
OT  - Therapy
EDAT- 2021/01/22 06:00
MHDA- 2021/05/20 06:00
CRDT- 2021/01/21 12:21
PHST- 2020/07/20 00:00 [received]
PHST- 2021/01/02 00:00 [accepted]
PHST- 2021/01/22 06:00 [pubmed]
PHST- 2021/05/20 06:00 [medline]
PHST- 2021/01/21 12:21 [entrez]
AID - 10.1007/s11033-021-06135-0 [pii]
AID - 10.1007/s11033-021-06135-0 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2021 Feb;48(2):1233-1241. doi: 10.1007/s11033-021-06135-0. Epub 
      2021 Jan 21.