PMID- 33476669
OWN - NLM
STAT- MEDLINE
DCOM- 20220207
LR  - 20220207
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 119
DP  - 2021 Jun
TI  - Molecular characterization, expression and function analysis of Epinephelus 
      coioides MKK4 response to SGIV and Vibrio alginolyticus infection.
PG  - 104020
LID - S0145-305X(21)00028-8 [pii]
LID - 10.1016/j.dci.2021.104020 [doi]
AB  - Mitogen-activated protein kinase 4 (MKK4), a member of the MAP kinase family, 
      play important roles in response to many environmental and cellular stresses in 
      mammals. In this study, three MKK4 subtypes, EcMKK4-1, EcMKK4-2 and EcMKK4-3, 
      were obtained from grouper Epinephelus coioides. The open reading frame (ORF) of 
      EcMKK4s are obtained and the EcMKK4s proteins contain highly conserved domains: a 
      S_TKc domain, a canonical diphosphorylation group and two conserved MKKK ATP 
      binding motifs, Asp-Phe-Gly (DFG) and Ala-Pro-Glu (APE). EcMKK4s could be found 
      both in the cytoplasmic and nuclear. The EcMKK4s mRNA were detected in all E. 
      coioides tissues examined with the different expression levels, and the 
      expression were up-regulated during SGIV (Singapore grouper iridescent virus) or 
      Vibrio alginolyticus infection. EcMKK4 could significantly reduce the activation 
      of AP-1 reporter gene. The results suggested that EcMKK4s might play important 
      roles in pathogen-caused inflammation.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Su, Yu-Ling
AU  - Su YL
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
FAU - Jiang, Yu-Feng
AU  - Jiang YF
AD  - Department of Laboratory, Jining No.1 People's Hospital; Postdoctoral Mobile 
      Station of Shandong University of Traditional Chinese Medicine, Shandong, 272111, 
      PR China.
FAU - Chen, He-Jia
AU  - Chen HJ
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
FAU - Ye, Shi
AU  - Ye S
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
FAU - Zhou, Wei-Hua
AU  - Zhou WH
AD  - Hainan Key Laboratory of Tropical Marine Biotechnology, South China Sea Institute 
      of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
FAU - Liu, Hong-Ping
AU  - Liu HP
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
FAU - Dong, Jun-De
AU  - Dong JD
AD  - Hainan Key Laboratory of Tropical Marine Biotechnology, South China Sea Institute 
      of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
FAU - Wei, Jing-Guang
AU  - Wei JG
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
FAU - Qin, Qi-Wei
AU  - Qin QW
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China. 
      Electronic address: qinqw@scau.edu.cn.
FAU - Sun, Hongyan
AU  - Sun H
AD  - Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine 
      Bioresource Conservation and Exploitation, College of Marine Sciences, South 
      China Agricultural University, Guangzhou 510642, Guangdong Province, PR China. 
      Electronic address: hongyanlucky@scau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210119
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/genetics/immunology
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Fish Diseases/*immunology/microbiology/virology
MH  - Fish Proteins/classification/genetics/*immunology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*immunology
MH  - Host-Pathogen Interactions/immunology
MH  - Iridovirus/*immunology/physiology
MH  - MAP Kinase Kinase 4/classification/genetics/*immunology
MH  - Perciformes/*immunology/microbiology/virology
MH  - Phylogeny
MH  - RNA, Messenger/genetics/immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Up-Regulation/immunology
MH  - Vibrio alginolyticus/*immunology/physiology
OTO - NOTNLM
OT  - Apoptosis
OT  - Epinephelus coioides
OT  - MKK4
OT  - SGIV
OT  - Vibrio alginolyticus
EDAT- 2021/01/22 06:00
MHDA- 2022/02/08 06:00
CRDT- 2021/01/21 20:10
PHST- 2020/10/03 00:00 [received]
PHST- 2021/01/13 00:00 [revised]
PHST- 2021/01/13 00:00 [accepted]
PHST- 2021/01/22 06:00 [pubmed]
PHST- 2022/02/08 06:00 [medline]
PHST- 2021/01/21 20:10 [entrez]
AID - S0145-305X(21)00028-8 [pii]
AID - 10.1016/j.dci.2021.104020 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2021 Jun;119:104020. doi: 10.1016/j.dci.2021.104020. Epub 2021 
      Jan 19.