PMID- 33478156
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 2
DP  - 2021 Jan 19
TI  - Simultaneous Determination of Cortisol, Cortisone, and Multiple Illicit Drugs in 
      Hair among Female Drug Addicts with LC-MS/MS.
LID - 10.3390/molecules26020516 [doi]
LID - 516
AB  - Long-term dependence of illicit drugs impairs the function of the 
      hypothalamic-pituitary-adrenal (HPA) axis, which regulates the secretion of 
      endogenous steroids, cortisol, and cortisone. Thus, the present study aimed to 
      develop a sensitive method for simultaneous determination of the multiple illicit 
      drugs and two steroids in hair to monitor the status of illicit drug exposure and 
      the physiological and psychological health of drug addicts. The target analytes 
      were extracted from hair by incubation with 1 mL methanol for 24 h at 40  degrees C and 
      then determined with LC-APCI(+)-MS/MS. The validated method showed acceptable 
      linearity (R (2) > 0.99) in the range of 1.25-250 pg/mg for cortisol and 
      cortisone, 2.5-125 pg/mg for heroin, 2.5-1250 pg/mg for ketamine, 2.5-5000 pg/mg 
      for methamphetamine (MAM), 2.5-250 pg/mg for 3, 4-methylenedioxymethamphetamine 
      (MDMA), morphine, and 6-monoacetylmorphine (6-AM). Limits of quantification were 
      1.6, 1.2, 1.6, 1.0, 1.4, 0.3, 2.1, and 1.2 pg/mg for cortisol, cortisone, heroin, 
      ketamine, MAM, MDMA, morphine, and 6-AM, respectively. Method recoveries were 
      from 90-115% for all analytes. Inter-day and intra-day coefficients of variation 
      were within 10%. Finally, this method was successfully applied to detect the 
      aforementioned analytes in hair among female drug addicts who self-reported to be 
      MAM abuser, heroin abuser, ketamine abuser, and abuser of mixture drugs of MAM 
      and heroin. MAM abusers with current MAM use showed significantly higher 
      concentrations of cortisol, MAM, and MDMA than controls with drug withdrawal.
FAU - Duan, Cailing
AU  - Duan C
AD  - Key Laboratory of Child Development and Learning Science, Ministry of Education, 
      Southeast University, Nanjing 210096, China.
AD  - Institute of Child Development and Education, Research Center of Learning 
      Science, Southeast University, Nanjing 210096, China.
AD  - Hangzhou College of Preschool Teacher Education, Zhejiang Normal University, 
      Hangzhou 311231, China.
FAU - Wu, Yan
AU  - Wu Y
AD  - Key Laboratory of Child Development and Learning Science, Ministry of Education, 
      Southeast University, Nanjing 210096, China.
AD  - Institute of Child Development and Education, Research Center of Learning 
      Science, Southeast University, Nanjing 210096, China.
FAU - Yang, Jin
AU  - Yang J
AD  - Key Laboratory of Child Development and Learning Science, Ministry of Education, 
      Southeast University, Nanjing 210096, China.
AD  - Institute of Child Development and Education, Research Center of Learning 
      Science, Southeast University, Nanjing 210096, China.
FAU - Chen, Shenghuo
AU  - Chen S
AD  - Key Laboratory of Child Development and Learning Science, Ministry of Education, 
      Southeast University, Nanjing 210096, China.
AD  - Institute of Child Development and Education, Research Center of Learning 
      Science, Southeast University, Nanjing 210096, China.
FAU - Pu, Yun
AU  - Pu Y
AD  - Women's Compulsory Isolated Drug Rehabilitation Center, Nanjing 210031, China.
FAU - Deng, Huihua
AU  - Deng H
AD  - Key Laboratory of Child Development and Learning Science, Ministry of Education, 
      Southeast University, Nanjing 210096, China.
AD  - Institute of Child Development and Education, Research Center of Learning 
      Science, Southeast University, Nanjing 210096, China.
LA  - eng
PT  - Journal Article
DEP - 20210119
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Illicit Drugs)
RN  - V27W9254FZ (Cortisone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - *Chromatography, Liquid
MH  - Cortisone/*analysis
MH  - Female
MH  - Hair/*chemistry
MH  - Humans
MH  - Hydrocortisone/*analysis
MH  - Illicit Drugs/*analysis
MH  - Limit of Detection
MH  - *Tandem Mass Spectrometry
PMC - PMC7835904
OTO - NOTNLM
OT  - LC-MS/MS
OT  - cortisol
OT  - cortisone
OT  - hair matrix
OT  - illicit drugs
COIS- The authors declare no conflict of interest. The funders has no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/01/23 06:00
MHDA- 2021/09/04 06:00
PMCR- 2021/01/19
CRDT- 2021/01/22 01:04
PHST- 2020/11/27 00:00 [received]
PHST- 2021/01/12 00:00 [revised]
PHST- 2021/01/16 00:00 [accepted]
PHST- 2021/01/22 01:04 [entrez]
PHST- 2021/01/23 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2021/01/19 00:00 [pmc-release]
AID - molecules26020516 [pii]
AID - molecules-26-00516 [pii]
AID - 10.3390/molecules26020516 [doi]
PST - epublish
SO  - Molecules. 2021 Jan 19;26(2):516. doi: 10.3390/molecules26020516.