PMID- 33478473 OWN - NLM STAT- MEDLINE DCOM- 20210712 LR - 20210712 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 17 IP - 1 DP - 2021 Jan 21 TI - S. pseudintermedius and S. aureus lineages with transmission ability circulate as causative agents of infections in pets for years. PG - 42 LID - 10.1186/s12917-020-02726-4 [doi] LID - 42 AB - BACKGROUND: Staphylococcus pseudintermedius (SP) and Staphylococcus aureus (SA) are common colonizers of companion animals, but they are also considered opportunistic pathogens, causing diseases of diverse severity. This study focused on the identification and characterization of 33 coagulase-positive staphylococci isolated from diseased pets (28 dogs and five cats) during 2009-2011 in a veterinary hospital in Spain in order to stablish the circulating lineages and their antimicrobial resistance profile. RESULTS: Twenty-eight isolates were identified as SP and five as SA. Nine methicillin-resistant (MR) isolates (27%) carrying the mecA gene were detected (eight MRSP and one MRSA). The 55% of SP and SA isolates were multidrug-resistant (MDR). MRSP strains were typed as ST71-agrIII-SCCmecII/III-(PFGE) A (n=5), ST68-agrIV-SCCmecV-B1/B2 (n=2), and ST258-agrII-SCCmecIV-C (n=1). SP isolates showed resistance to the following antimicrobials [percentage of resistant isolates/resistance genes]: penicillin [82/blaZ], oxacillin [29/mecA] erythromycin/clindamycin [43/erm(B)], aminoglycosides [18-46/aacA-aphD, aphA3, aadE], tetracycline [71/tet(M), tet(K)], ciprofloxacin [29], chloramphenicol [29/cat(pC221)], and trimethoprim-sulfamethoxazole [50/dfrG, dfrK]. The dfrK gene was revealed as part of the radC-integrated Tn559 in two SP isolates. Virulence genes detected among SP isolates were as follow [percentage of isolates]: siet [100], se-int [100], lukS/F-I [100], sec(canine) [7], and expB [7]. The single MRSA-mecA detected was typed as t011-ST398/CC398-agrI-SCCmecV and was MDR. The methicillin-susceptible SA isolates were typed as t045-ST5/CC5 (n=2), t10576-ST1660 (n=1), and t005-ST22/CC22 (n=1); the t005-ST22 feline isolate was PVL-positive and the two t045-ST45 isolates were ascribed to Immune Evasion Cluster (IEC) type F. Moreover, the t10576-ST1660 isolate, of potential equine origin, harbored the lukPQ and scneq genes. According to animal clinical history and data records, several strains seem to have been acquired from different sources of the hospital environment, while some SA strains appeared to have a human origin. CONCLUSIONS: The frequent detection of MR and MDR isolates among clinical SP and SA strains with noticeable virulence traits is of veterinary concern, implying limited treatment options available. This is the first description of MRSA-ST398 and MRSP-ST68 in pets in Spain, as well the first report of the dfrK-carrying Tn559 in SP. This evidences that current transmissible lineages with mobilizable resistomes have been circulating as causative agents of infections among pets for years. FAU - Ruiz-Ripa, Laura AU - Ruiz-Ripa L AD - Area Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, 26006, Spain. FAU - Simon, Carmen AU - Simon C AD - Departamento de Patologia Animal, Universidad de Zaragoza, Zaragoza, 50013, Spain. FAU - Ceballos, Sara AU - Ceballos S AD - Area Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, 26006, Spain. FAU - Ortega, Carmelo AU - Ortega C AD - Departamento de Patologia Animal, Universidad de Zaragoza, Zaragoza, 50013, Spain. FAU - Zarazaga, Myriam AU - Zarazaga M AD - Area Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, 26006, Spain. FAU - Torres, Carmen AU - Torres C AD - Area Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, 26006, Spain. FAU - Gomez-Sanz, Elena AU - Gomez-Sanz E AUID- ORCID: 0000-0001-7666-3642 AD - Area Bioquimica y Biologia Molecular, Universidad de La Rioja, Logrono, 26006, Spain. elena.gomez@hest.ethz.ch. AD - Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, LFV B36, 8092, Zurich, Switzerland. elena.gomez@hest.ethz.ch. AD - Area de Microbiologia Molecular, Centro de Investigacion Biomedica de La Rioja (CIBIR), Logrono, 26006, Spain. elena.gomez@hest.ethz.ch. LA - eng GR - SAF2012-35474/Agencia Estatal de Investigacion (AEI) of Spain/ GR - SAF2016-76571-R/Fondo Europeo de Desarrollo Regional (FEDER) of EU/ GR - 167090/Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung/ GR - 659314/H2020 Marie Sklodowska-Curie Actions (MSCA-FI-GF)/ PT - Journal Article DEP - 20210121 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 RN - 0 (Anti-Bacterial Agents) RN - Staphylococcus pseudintermedius SB - IM MH - Animals MH - Anti-Bacterial Agents/pharmacology MH - Carrier State/microbiology/veterinary MH - Cat Diseases/epidemiology/microbiology MH - Cats MH - Dog Diseases/epidemiology/microbiology MH - Dogs MH - Drug Resistance, Multiple, Bacterial MH - Methicillin Resistance/genetics MH - Methicillin-Resistant Staphylococcus aureus/genetics/isolation & purification MH - Microbial Sensitivity Tests/veterinary MH - Pets MH - Spain MH - Staphylococcal Infections/epidemiology/*veterinary MH - Staphylococcus/genetics/*isolation & purification MH - Staphylococcus aureus/genetics/*isolation & purification MH - Virulence/genetics PMC - PMC7819200 OTO - NOTNLM OT - Infection OT - MRSA OT - MRSA-CC398 OT - MRSP OT - MRSP-ST71 OT - Pets OT - Tn559 COIS- The authors declare that they have no competing interests. EDAT- 2021/01/23 06:00 MHDA- 2021/07/13 06:00 PMCR- 2021/01/21 CRDT- 2021/01/22 05:45 PHST- 2020/04/23 00:00 [received] PHST- 2020/12/21 00:00 [accepted] PHST- 2021/01/22 05:45 [entrez] PHST- 2021/01/23 06:00 [pubmed] PHST- 2021/07/13 06:00 [medline] PHST- 2021/01/21 00:00 [pmc-release] AID - 10.1186/s12917-020-02726-4 [pii] AID - 2726 [pii] AID - 10.1186/s12917-020-02726-4 [doi] PST - epublish SO - BMC Vet Res. 2021 Jan 21;17(1):42. doi: 10.1186/s12917-020-02726-4.