PMID- 33479910
OWN - NLM
STAT- MEDLINE
DCOM- 20210402
LR  - 20210402
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2270
DP  - 2021
TI  - Regulatory and IgE(+) B Cells in Allergic Asthma.
PG  - 375-418
LID - 10.1007/978-1-0716-1237-8_21 [doi]
AB  - Allergic asthma is triggered by inhalation of environmental allergens resulting 
      in bronchial constriction and inflammation, which leads to clinical symptoms such 
      as wheezing, coughing, and difficulty breathing. Asthmatic airway inflammation is 
      initiated by inflammatory mediators released by granulocytic cells. However, the 
      immunoglobulin E (IgE) antibody is necessary for the initiation of the allergic 
      cascade, and IgE is produced and released exclusively by memory B cells and 
      plasma cells. Acute allergen exposure has also been shown to increase IgE levels 
      in the airways of patients diagnosed with allergic asthma; however, more studies 
      are needed to understand local airway inflammation. Additionally, regulatory B 
      cells (B(regs)) have been shown to modulate IgE-mediated inflammatory processes 
      in allergic asthma pathogenesis, particularly in mouse models of allergic airway 
      disease. However, the levels and function of these IgE(+) B cells and B(regs) 
      remain to be elucidated in human models of asthma. The overall objective for this 
      chapter is to provide detailed methodological, and insightful technological 
      advances to study the biology of B cells in allergic asthma pathogenesis. 
      Specifically, we will describe how to investigate the frequency and function of 
      IgE(+) B cells and B(regs) in allergic asthma, and the kinetics of these cells 
      after allergen exposure in a human asthma model.
FAU - Oliveria, John Paul
AU  - Oliveria JP
AD  - School of Medicine, Department of Pathology, Stanford University, Stanford, CA, 
      USA.
AD  - Department of Medicine, Division of Respirology, McMaster University, Hamilton, 
      ON, Canada.
FAU - Agayby, Rita
AU  - Agayby R
AD  - Department of Medicine, Division of Respirology, McMaster University, Hamilton, 
      ON, Canada.
FAU - Gauvreau, Gail M
AU  - Gauvreau GM
AD  - Department of Medicine, Division of Respirology, McMaster University, Hamilton, 
      ON, Canada. gauvreau@mcmaster.ca.
LA  - eng
GR  - CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology
MH  - Asthma/immunology/metabolism
MH  - B-Lymphocytes/*cytology/immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/immunology/*pathology
MH  - Immunoglobulin E/*immunology/metabolism
MH  - Inflammation/pathology
MH  - Lung/immunology
MH  - Male
MH  - Models, Biological
OTO - NOTNLM
OT  - Airway inflammation
OT  - Allergic asthma
OT  - Human immunology
OT  - IgE+ B cells
OT  - Inhaled allergen challenge
OT  - Regulatory B cells
OT  - Sputum
EDAT- 2021/01/23 06:00
MHDA- 2021/04/07 06:00
CRDT- 2021/01/22 06:29
PHST- 2021/01/22 06:29 [entrez]
PHST- 2021/01/23 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
AID - 10.1007/978-1-0716-1237-8_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2270:375-418. doi: 10.1007/978-1-0716-1237-8_21.