PMID- 33482190
OWN - NLM
STAT- MEDLINE
DCOM- 20210226
LR  - 20210226
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 269
DP  - 2021 Mar 15
TI  - The MIR155 host gene/microRNA-627/HMGB1/NF-kappaB loop modulates fibroblast 
      proliferation and extracellular matrix deposition.
PG  - 119085
LID - S0024-3205(21)00070-9 [pii]
LID - 10.1016/j.lfs.2021.119085 [doi]
AB  - Pulmonary fibrosis (PF), which is characterized by excessive matrix formation, 
      may ultimately lead to irreversible lung damage and thus death. Fibroblast 
      activation has been regarded as a central event during PF pathogenesis. In our 
      previous study, we confirmed that the miR-627/high-mobility group box protein 1 
      (HMGB1)/Nuclear factor kappa beta (NF-kappaB) axis modulates transforming growth 
      factor beta 1 (TGFbeta1)-induced pulmonary fibrosis. In the present study, we 
      investigated the upstream factors leading to miR-627 dysregulation in the process 
      of pulmonary fibroblast activation and PF. The lncRNA MIR155 host gene (MIR155HG) 
      was found to be abnormally upregulated in pulmonary fibrosis tissues and 
      TGFbeta1-stimulated normal human primary lung fibroblasts (NHLFs). By directly 
      binding to miR-627, MIR155HG inhibited miR-627 expression. MIR155HG 
      overexpression enhanced TGFbeta1-induced increases in HMGB1 protein expression and 
      p65 phosphorylation, NHLF proliferation, and extracellular matrix (ECM) 
      deposition. In contrast, miR-627 overexpression attenuated the TGFbeta1-induced 
      changes in NHLFs and significantly reversed the effects of MIR155HG 
      overexpression. Under TGFbeta1 stimulation, miR-627 inhibition promoted, whereas 
      JSH-23 treatment inhibited NF-kappaB activation; in NHLFs, NF-kappaB overexpression 
      upregulated, whereas JSH-23 treatment downregulated MIR155HG expression. In 
      tissue samples, HMGB1 protein levels and p65 phosphorylation were increased; 
      MIR155HG was negatively correlated with miR-627 and positively correlated with 
      HMGB1. In conclusion, we validated that the MIR155HG/miR-627/HMGB1/NF-kappaB axis 
      formed a regulatory loop that modulates TGFbeta1-induced NHLF activation. 
      Considering the critical role of NHLF activation in PF pathogenesis, the 
      NF-kappaB/MIR155HG/miR-627/HMGB1 regulatory loop could exert a vital effect on PF 
      pathogenesis. Further in vivo and clinical investigations are required to confirm 
      this model.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Li, Jie
AU  - Li J
AD  - Department of Internal Medicine, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Zhang, Xueyu
AU  - Zhang X
AD  - Department of Internal Medicine, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Wang, Tao
AU  - Wang T
AD  - Department of Thoracic Surgery, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Li, Jinghong
AU  - Li J
AD  - Department of Internal Medicine, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Su, Qi
AU  - Su Q
AD  - Medical Department, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Zhong, Cheng
AU  - Zhong C
AD  - Department of Internal Medicine, Jiangxi Chest Hospital, Nanchang 330006, China.
FAU - Chen, Zhongshu
AU  - Chen Z
AD  - Department of Thoracic Surgery, Jiangxi Chest Hospital, Nanchang 330006, China. 
      Electronic address: czs680819@126.com.
FAU - Liang, Ying
AU  - Liang Y
AD  - Department of Food Science and Engineering, Central South University of Forestry 
      and Technology, Changsha, 410004, Hunan, China. Electronic address: 
      Liangying498@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210120
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MIRN627 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Case-Control Studies
MH  - Cell Proliferation
MH  - Extracellular Matrix/*metabolism
MH  - Fibroblasts/*cytology/metabolism
MH  - Gene Expression Regulation
MH  - HMGB1 Protein/genetics/*metabolism
MH  - Humans
MH  - Lung/cytology/metabolism
MH  - MicroRNAs/*genetics
MH  - NF-kappa B/genetics/*metabolism
MH  - Pulmonary Fibrosis/genetics/metabolism/*pathology
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction
MH  - Transforming Growth Factor beta1/genetics/metabolism
OTO - NOTNLM
OT  - Fibroblast activation
OT  - High-mobility group box protein 1 (HMGB1)
OT  - MIR155 host gene (MIR155HG)
OT  - Nuclear factor kappa beta (NF-kappaB)
OT  - Pulmonary fibrosis (PF)
OT  - miR-627
EDAT- 2021/01/23 06:00
MHDA- 2021/02/27 06:00
CRDT- 2021/01/22 20:09
PHST- 2020/10/30 00:00 [received]
PHST- 2021/01/08 00:00 [revised]
PHST- 2021/01/12 00:00 [accepted]
PHST- 2021/01/23 06:00 [pubmed]
PHST- 2021/02/27 06:00 [medline]
PHST- 2021/01/22 20:09 [entrez]
AID - S0024-3205(21)00070-9 [pii]
AID - 10.1016/j.lfs.2021.119085 [doi]
PST - ppublish
SO  - Life Sci. 2021 Mar 15;269:119085. doi: 10.1016/j.lfs.2021.119085. Epub 2021 Jan 
      20.