PMID- 33482202
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 173
DP  - 2021 Mar 15
TI  - A novel bifunctional glucanase exhibiting high production of glucose and 
      cellobiose from rumen bacterium.
PG  - 136-145
LID - S0141-8130(21)00145-8 [pii]
LID - 10.1016/j.ijbiomac.2021.01.113 [doi]
AB  - Herbivores gastrointestinal microbiota is of tremendous interest for mining novel 
      lignocellulosic enzymes for bioprocessing. We previously reported a set of 
      potential carbohydrate-active enzymes from the metatranscriptome of the Hu sheep 
      rumen microbiome. In this study, we isolated and heterologously expressed two 
      novel glucanase genes, Cel5A-h38 and Cel5A-h49, finding that both recombinant 
      enzymes showed the optimum temperatures of 50  degrees C. Substrate-specificity 
      determination revealed that Cel5A-h38 was exclusively active in the presence of 
      mixed-linked glucans, such as barley beta-glucan and Icelandic moss lichenan, 
      whereas Cel5A-h49 (EC 3.2.1.4) exhibited a wider substrate spectrum. 
      Surprisingly, Cel5A-h38 initially released only cellotriose from lichenan and 
      further converted it into an equivalent amount of glucose and cellobiose, 
      suggesting a dual-function as both endo-beta-1,3-1,4-glucanase (EC 3.2.1.73) and 
      exo-cellobiohydrolase (EC 3.2.1.91). Additionally, we performed enzymatic 
      hydrolysis of sheepgrass (Leymus chinensis) and rice (Orysa sativa) straw using 
      Cel5A-h38, revealing liberation of 1.91 +/- 0.30 mmol/mL and 2.03 +/- 0.09 mmol/mL 
      reducing sugars, respectively, including high concentrations of glucose and 
      cellobiose. These results provided new insights into glucanase activity and lay a 
      foundation for bioconversion of lignocellulosic biomass.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Cao, Jia-Wen
AU  - Cao JW
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China; Institute of Dairy Science, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Deng, Qian
AU  - Deng Q
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China; Institute of Dairy Science, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Gao, De-Ying
AU  - Gao DY
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China; Institute of Dairy Science, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, China; College of Biological and 
      Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China.
FAU - He, Bo
AU  - He B
AD  - State Key Laboratory of Microbial Metabolism, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Yin, Shang-Jun
AU  - Yin SJ
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo 315100, China.
FAU - Qian, Li-Chun
AU  - Qian LC
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China.
FAU - Wang, Jia-Kun
AU  - Wang JK
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China; Institute of Dairy Science, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: 
      jiakunwang@zju.edu.cn.
FAU - Wang, Qian
AU  - Wang Q
AD  - Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang 
      University, Hangzhou 310058, China; Institute of Dairy Science, College of Animal 
      Sciences, Zhejiang University, Hangzhou 310058, China; College of Biological and 
      Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China. 
      Electronic address: Emirate14@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210119
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Bacterial Proteins)
RN  - 0 (Glucans)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Trioses)
RN  - 0 (beta-Glucans)
RN  - 0 (cellotriose)
RN  - 16462-44-5 (Cellobiose)
RN  - 9004-34-6 (Cellulose)
RN  - EC 3.2.1.6 (Endo-1,3(4)-beta-Glucanase)
RN  - EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
RN  - IY9XDZ35W2 (Glucose)
RN  - M7F3LRN53I (lichenin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Cellobiose/*biosynthesis
MH  - Cellulose/metabolism
MH  - Cellulose 1,4-beta-Cellobiosidase/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Endo-1,3(4)-beta-Glucanase/genetics/*metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Gastrointestinal Microbiome/physiology
MH  - Gene Expression
MH  - Genetic Vectors/chemistry/metabolism
MH  - Glucans/metabolism
MH  - Glucose/*biosynthesis
MH  - Hydrolysis
MH  - Kinetics
MH  - Recombinant Proteins/genetics/metabolism
MH  - Rumen/microbiology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sheep/microbiology
MH  - Substrate Specificity
MH  - Trioses/metabolism
MH  - beta-Glucans/metabolism
OTO - NOTNLM
OT  - Cello-oligosaccharides
OT  - Glucanase
OT  - Hydrolysis
OT  - Natural feedstocks
OT  - Rumen microbe
COIS- Declaration of competing interest The authors declared that they have no 
      conflicts of interest to this work.
EDAT- 2021/01/23 06:00
MHDA- 2021/05/01 06:00
CRDT- 2021/01/22 20:09
PHST- 2020/09/16 00:00 [received]
PHST- 2020/12/21 00:00 [revised]
PHST- 2021/01/17 00:00 [accepted]
PHST- 2021/01/23 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2021/01/22 20:09 [entrez]
AID - S0141-8130(21)00145-8 [pii]
AID - 10.1016/j.ijbiomac.2021.01.113 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 Mar 15;173:136-145. doi: 
      10.1016/j.ijbiomac.2021.01.113. Epub 2021 Jan 19.