PMID- 33482557
OWN - NLM
STAT- MEDLINE
DCOM- 20210318
LR  - 20210318
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 769
DP  - 2021 May 15
TI  - Identification of cable bacteria and its biogeochemical impact on sulfur in 
      freshwater sediments from the Wenyu River.
PG  - 144541
LID - S0048-9697(20)38072-4 [pii]
LID - 10.1016/j.scitotenv.2020.144541 [doi]
AB  - Cable bacteria are filamentous sulfur-oxidizing microorganisms that couple the 
      reduction of oxygen or nitrate in surface sediments with the oxidation of free 
      sulfide in deeper sediments by transferring electrons across centimeter scale 
      distances. The distribution and activities of cable bacteria in freshwater 
      sediments are still poorly understood, especially the impact of cable bacteria on 
      sulfur cycling. The goal of this study was to investigate electrogenic sulfide 
      oxidation associated with cable bacteria in laboratory microcosm incubations of 
      freshwater sediments using microsensor technology, 16S full-length rRNA 
      sequencing, and fluorescence in situ hybridization (FISH) microscopy. Their 
      activity was characterized by a pH maximum of 8.56 in the oxic zone and the 
      formation of a 13.7 +/- 0.6 mm wide suboxic zone after 25 days of incubation. 
      Full-length 16S rRNA gene sequences related to cable bacteria were recovered from 
      the sediments and exhibited 93.3%-99.4% nucleotide (nt) similarities with those 
      from other reported freshwater cable bacteria, indicating that new species of 
      cable bacteria were present in the sediments. FISH analysis indicated that cable 
      bacteria density increased with time, reaching a maximum of 95.48 m cm(-2) on day 
      50. The cells grew downwards to 40 mm but were mainly concentrated on the top 
      0-20 mm of sediment. The cable bacteria continuously consumed H(2)S in deeper 
      layers and oxidized sulfide into sulfate in the 0-20 mm surface layers, thereby 
      affecting the sulfur cycling within sediments. These findings provide new 
      evidence for the existence of higher diversity of cable bacteria in freshwater 
      sediments than previously known.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Xu, Xiaoling
AU  - Xu X
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Science, Beijing 100012, China; College of 
      Water Sciences, Beijing Normal University, Beijing 100012, China.
FAU - Huo, Shouliang
AU  - Huo S
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Science, Beijing 100012, China. Electronic 
      address: huosl@craes.org.cn.
FAU - Zhang, Hanxiao
AU  - Zhang H
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Science, Beijing 100012, China; College of 
      Water Sciences, Beijing Normal University, Beijing 100012, China.
FAU - Li, Xiaochuang
AU  - Li X
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Science, Beijing 100012, China.
FAU - Wu, Fengchang
AU  - Wu F
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Science, Beijing 100012, China.
LA  - eng
PT  - Journal Article
DEP - 20210114
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 70FD1KFU70 (Sulfur)
SB  - IM
MH  - Bacteria/genetics
MH  - *Deltaproteobacteria/genetics
MH  - Fresh Water
MH  - Geologic Sediments
MH  - In Situ Hybridization, Fluorescence
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rivers
MH  - Sulfur
OTO - NOTNLM
OT  - Biogeochemical cycling
OT  - Cable bacteria
OT  - Electrogenic activity
OT  - Sulfide oxidation
OT  - Sulfur
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/01/23 06:00
MHDA- 2021/03/19 06:00
CRDT- 2021/01/22 20:14
PHST- 2020/08/28 00:00 [received]
PHST- 2020/11/25 00:00 [revised]
PHST- 2020/12/14 00:00 [accepted]
PHST- 2021/01/23 06:00 [pubmed]
PHST- 2021/03/19 06:00 [medline]
PHST- 2021/01/22 20:14 [entrez]
AID - S0048-9697(20)38072-4 [pii]
AID - 10.1016/j.scitotenv.2020.144541 [doi]
PST - ppublish
SO  - Sci Total Environ. 2021 May 15;769:144541. doi: 10.1016/j.scitotenv.2020.144541. 
      Epub 2021 Jan 14.