PMID- 33482765
OWN - NLM
STAT- MEDLINE
DCOM- 20210511
LR  - 20210511
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jan 22
TI  - Raptor and rictor expression in patients with human papillomavirus-related 
      oropharyngeal squamous cell carcinoma.
PG  - 87
LID - 10.1186/s12885-021-07794-9 [doi]
LID - 87
AB  - BACKGROUND: Despite reports of a link between human papillomavirus (HPV) 
      infection and mechanistic target of rapamycin (mTOR) signaling activation, the 
      role of the mTOR pathway, especially raptor and rictor, in HPV-related head and 
      neck cancer is still unclear. The aim of the present study was to elucidate the 
      role of the mTOR pathway in HPV-related oropharyngeal squamous cell carcinoma 
      (OPSCC). METHODS: The present study involved two strategies. The first was to 
      investigate the activity of mTOR and mTOR-related complexes in high-risk 
      HPV-positive (UM-SCC47 and CaSki) and HPV-negative (SCC-4 and SAS) cancer cell 
      lines. The second was to elucidate mTOR complex expression in 80 oropharyngeal 
      cancer tissues and to examine the relationship between mTOR complex expression 
      and survival in patients with OPSCC. RESULTS: The UM-SCC47 and CaSki cell lines 
      showed high gene and protein expression of raptor. They also exhibited G1/S and 
      G2/M phase cell cycle arrest following 24 h incubation with 6 muM temsirolimus, a 
      rapamycin analog, and temsirolimus administration inhibited their growth. 
      HPV-related OPSCC samples showed high gene and protein expression of raptor and 
      rictor compared with HPV-unrelated OPSCC. In addition, HPV-related OPSCC patients 
      with high raptor and rictor expression tended to have a worse prognosis than 
      those with low or medium expression. CONCLUSIONS: These results suggest that 
      raptor and rictor have important roles in HPV-related OPSCC and that temsirolimus 
      is a potential therapeutic agent for patients with HPV-related OPSCC. This is the 
      first report to reveal the overexpression of raptor and rictor in HPV-related 
      OPSCC.
FAU - Kondo, Shunsuke
AU  - Kondo S
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Hirakawa, Hitoshi
AU  - Hirakawa H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Ikegami, Taro
AU  - Ikegami T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Uehara, Takayuki
AU  - Uehara T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Agena, Shinya
AU  - Agena S
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Uezato, Jin
AU  - Uezato J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Kinjyo, Hidetoshi
AU  - Kinjyo H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Kise, Noritomo
AU  - Kise N
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Yamashita, Yukashi
AU  - Yamashita Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Tanaka, Katsunori
AU  - Tanaka K
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Hasegawa, Narumi
AU  - Hasegawa N
AD  - Ryukyu Society for the Promotion of Oto-Rhino-Laryngology, Nishihara-cho, 
      Okinawa, 903-0215, Japan.
FAU - Kiyuna, Asanori
AU  - Kiyuna A
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Maeda, Hiroyuki
AU  - Maeda H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
FAU - Suzuki, Mikio
AU  - Suzuki M
AUID- ORCID: 0000-0001-7743-8666
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan. suzuki@med.u-ryukyu.ac.jp.
FAU - Gahana, Akira
AU  - Gahana A
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of 
      Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 
      903-0215, Japan.
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, University of Miyazaki, 
      Miyazaki, 889-1692, Japan.
LA  - eng
GR  - 168F010007/Japan Society for the Promotion of Science/
GR  - 17K11390/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20210122
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RICTOR protein, human)
RN  - 0 (RPTOR protein, human)
RN  - 0 (Rapamycin-Insensitive Companion of mTOR Protein)
RN  - 0 (Regulatory-Associated Protein of mTOR)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Carcinoma, Squamous Cell/epidemiology/metabolism/*pathology/virology
MH  - Cell Cycle
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Japan/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Oropharyngeal Neoplasms/epidemiology/metabolism/*pathology/virology
MH  - Papillomaviridae/*isolation & purification
MH  - Papillomavirus Infections/*complications/virology
MH  - Prognosis
MH  - Rapamycin-Insensitive Companion of mTOR Protein/*metabolism
MH  - Regulatory-Associated Protein of mTOR/*metabolism
MH  - Survival Rate
MH  - Tumor Cells, Cultured
PMC - PMC7821513
OTO - NOTNLM
OT  - Human papillomavirus
OT  - Oropharyngeal cancer
OT  - Overall survival
OT  - Rapalog
OT  - Raptor
OT  - Rictor
OT  - Temsirolimus
OT  - mTOR
COIS- The authors declare that they have no competing interests.
EDAT- 2021/01/24 06:00
MHDA- 2021/05/12 06:00
PMCR- 2021/01/22
CRDT- 2021/01/23 05:25
PHST- 2020/03/10 00:00 [received]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2021/01/23 05:25 [entrez]
PHST- 2021/01/24 06:00 [pubmed]
PHST- 2021/05/12 06:00 [medline]
PHST- 2021/01/22 00:00 [pmc-release]
AID - 10.1186/s12885-021-07794-9 [pii]
AID - 7794 [pii]
AID - 10.1186/s12885-021-07794-9 [doi]
PST - epublish
SO  - BMC Cancer. 2021 Jan 22;21(1):87. doi: 10.1186/s12885-021-07794-9.