PMID- 33483310
OWN - NLM
STAT- MEDLINE
DCOM- 20210407
LR  - 20210513
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 87
IP  - 7
DP  - 2021 Mar 11
TI  - New Endohyphal Relationships between Mucoromycota and Burkholderiaceae 
      Representatives.
LID - 10.1128/AEM.02707-20 [doi]
LID - e02707-20
AB  - Mucoromycota representatives are known to harbor two types of endohyphal bacteria 
      (EHB)-Burkholderia-related endobacteria (BRE) and Mycoplasma-related endobacteria 
      (MRE). While both BRE and MRE occur in fungi representing all subphyla of 
      Mucoromycota, their distribution is not well studied. Therefore, it is difficult 
      to resolve the evolutionary history of these associations in favor of one of the 
      following two alternative hypotheses explaining their origin: "early invasion" 
      and "late invasion." Our main goal was to fill this knowledge gap by surveying 
      Mucoromycota fungi for the presence of EHB. We screened 196 fungal strains from 
      16 genera using a PCR-based approach to detect bacterial 16S rRNA genes, 
      complemented with fluorescence in situ hybridization (FISH) imaging to confirm 
      the presence of bacteria within the hyphae. We detected Burkholderiaceae in ca. 
      20% of fungal strains. Some of these bacteria clustered phylogenetically with 
      previously described BRE clades, whereas others grouped with free-living 
      Paraburkholderia Importantly, the latter were detected in Umbelopsidales, which 
      previously were not known to harbor endobacteria. Our results suggest that this 
      group of EHB is recruited from the environment, supporting the late invasion 
      scenario. This pattern complements the early invasion scenario apparent in the 
      BRE clade of EHB.IMPORTANCE Bacteria living within fungal hyphae present an 
      example of one of the most intimate relationships between fungi and bacteria. 
      Even though there are several well-described examples of such partnerships, their 
      prevalence within the fungal kingdom remains unknown. Our study focused on early 
      divergent terrestrial fungi in the phylum Mucoromycota. We found that ca. 20% of 
      the strains tested harbored bacteria from the family Burkholderiaceae Not only 
      did we confirm the presence of bacteria from previously described endosymbiont 
      clades, we also identified a new group of endohyphal Burkholderiaceae 
      representing the genus Paraburkholderia We established that more than half of the 
      screened Umbelopsis strains were positive for bacteria from this new group. We 
      also determined that, while previously described BRE codiverged with their fungal 
      hosts, Paraburkholderia symbionts did not.
CI  - Copyright (c) 2021 Okrasinska et al.
FAU - Okrasinska, Alicja
AU  - Okrasinska A
AUID- ORCID: 0000-0002-3402-1526
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland alis.ok@biol.uw.edu.pl.
FAU - Bokus, Aleksandra
AU  - Bokus A
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
FAU - Duk, Katarzyna
AU  - Duk K
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
FAU - Gesiorska, Aleksandra
AU  - Gesiorska A
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
FAU - Sokolowska, Blanka
AU  - Sokolowska B
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
FAU - Milobedzka, Aleksandra
AU  - Milobedzka A
AUID- ORCID: 0000-0002-3912-7581
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
AD  - Department of Water Technology and Environmental Engineering, University of 
      Chemistry and Technology Prague, Prague, Czech Republic.
FAU - Wrzosek, Marta
AU  - Wrzosek M
AUID- ORCID: 0000-0002-5871-5020
AD  - Botanic Garden, Faculty of Biology, University of Warsaw, Warsaw, Poland.
FAU - Pawlowska, Julia
AU  - Pawlowska J
AUID- ORCID: 0000-0003-4914-5182
AD  - Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw, 
      Warsaw, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210311
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Burkholderiaceae/*physiology
MH  - Fungi/*physiology
MH  - Hyphae/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/analysis
PMC - PMC8091615
OTO - NOTNLM
OT  - BRE
OT  - Mortierella
OT  - Umbelopsis
OT  - bacterial-fungal interactions
OT  - endosymbionts
EDAT- 2021/01/24 06:00
MHDA- 2021/04/10 06:00
PMCR- 2021/03/11
CRDT- 2021/01/23 05:33
PHST- 2020/11/03 00:00 [received]
PHST- 2021/01/09 00:00 [accepted]
PHST- 2021/01/24 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2021/01/23 05:33 [entrez]
PHST- 2021/03/11 00:00 [pmc-release]
AID - AEM.02707-20 [pii]
AID - 02707-20 [pii]
AID - 10.1128/AEM.02707-20 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2021 Mar 11;87(7):e02707-20. doi: 10.1128/AEM.02707-20. 
      Print 2021 Mar 11.