PMID- 33484738
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20240226
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 747
DP  - 2021 Mar 16
TI  - Enolase2 and enolase1 cooperate against neuronal injury in stroke model.
PG  - 135662
LID - S0304-3940(21)00040-9 [pii]
LID - 10.1016/j.neulet.2021.135662 [doi]
AB  - Stroke is one of the leading causes of death in adults worldwide. However, the 
      mechanism causing neuronal death remains poorly understood. Our previous report 
      showed that enolase1 (ENO1), a key glycolytic enzyme, alleviates cerebral 
      ischemia-induced neuronal injury. It remained unclear whether enolase2 (ENO2) 
      affects neuronal injury in stroke models. Here, we examined the effects of ENO2 
      in several stroke models. The results showed that the expression level of ENO2 
      was downregulated after 3 h of cerebral ischemia by middle cerebral artery 
      occlusion (MCAO) in the mouse model. ENO2 was expressed in mouse brain and 
      cultured hippocampus neurons. Overexpression of ENO2 in cultured hippocampus 
      neurons did not affect neuronal injury in our oxygen-glucose deprivation (OGD) 
      model. Interestingly, double knock-down (KD) of ENO1 and ENO2 increased neuronal 
      injury while either KD of ENO1 or ENO2 failed to increase neuronal injury in OGD. 
      Deletion of ENO1 did not affect anoxia-starvation (AS)-induced worm death in C. 
      elegans. These findings demonstrated that ENO2 and ENO1 work together against 
      neuronal injury in these stroke models.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Jiang, Wei
AU  - Jiang W
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Stingelin, Lukas
AU  - Stingelin L
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Zhang, Pei
AU  - Zhang P
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Tian, Xibin
AU  - Tian X
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Kang, Na
AU  - Kang N
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Liu, Junqiang
AU  - Liu J
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Aihemaiti, Yilixiati
AU  - Aihemaiti Y
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China.
FAU - Zhou, Desheng
AU  - Zhou D
AD  - Department of Neurology, First Affiliated Hospital of Hunan University of Chinese 
      Medicine, Changsha, Hunan, 410007, China. Electronic address: 
      zds1101@foxmail.com.
FAU - Tu, Haijun
AU  - Tu H
AD  - Institute of Neuroscience, State Key Laboratory of Chemo/Biosensing and 
      Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, 
      China. Electronic address: haijuntu@hnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210120
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain Injuries/*metabolism/pathology
MH  - Brain Ischemia/*metabolism/pathology
MH  - Disease Models, Animal
MH  - Infarction, Middle Cerebral Artery/metabolism
MH  - Mice, Inbred C57BL
MH  - Neurons/*metabolism
MH  - Phosphopyruvate Hydratase/*metabolism
MH  - Stroke/*metabolism/pathology
MH  - Mice
OTO - NOTNLM
OT  - AS
OT  - Cerebral ischemia
OT  - ENO2
OT  - OGD
OT  - Stroke model
EDAT- 2021/01/24 06:00
MHDA- 2021/06/04 06:00
CRDT- 2021/01/23 20:08
PHST- 2020/10/26 00:00 [received]
PHST- 2020/12/30 00:00 [revised]
PHST- 2021/01/13 00:00 [accepted]
PHST- 2021/01/24 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
PHST- 2021/01/23 20:08 [entrez]
AID - S0304-3940(21)00040-9 [pii]
AID - 10.1016/j.neulet.2021.135662 [doi]
PST - ppublish
SO  - Neurosci Lett. 2021 Mar 16;747:135662. doi: 10.1016/j.neulet.2021.135662. Epub 
      2021 Jan 20.