PMID- 33486279
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20211112
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 86
DP  - 2021 Apr
TI  - hucMSCs transplantation promotes locomotor function recovery, reduces apoptosis 
      and inhibits demyelination after SCI in rats.
PG  - 102125
LID - S0143-4179(21)00011-1 [pii]
LID - 10.1016/j.npep.2021.102125 [doi]
AB  - AIMS: Spinal cord injury (SCI) can cause a variety of cells apoptosis, 
      neurodegeneration, and eventually permanent paralysis. This study aimed to 
      examine whether transplanting human umbilical cord mesenchymal stem cells 
      (hucMSCs) can promote locomotor function recovery, reduce apoptosis and inhibit 
      demyelination in SCI models. MAIN METHODS: Rats were allocated into Sham group 
      (spinal cord exposure only), SCI + PBS group (spinal cord impact plus 
      phosphate-buffered saline (PBS) injections), SCI + hucMSCs group (spinal cord 
      impact plus hucMSCs injections) groups. Behavioral tests, Basso-Beattie-Bresnahan 
      locomotion scores (BBB scores), were carried out at 0, 3, 7, 14, 21, 28 days 
      after SCI surgery. Hematoxylin-eosin staining observed spinal cord morphology. 
      Nissl staining detected the number of nissl bodies. Myelin basic protein (MBP) 
      and oligodendrocyte (CNPase) were examed by immunohistochemical staining. The 
      apoptosis of oligodendrocyte and neurons were detected by immunofluorescence. 
      RESULTS: The 28-day behavioral test showed that the BBB score of rats in the 
      SCI + hucMSCs group increased significantly, comparing to the SCI + PBS group. 
      The numbers of nissl bodies and myelin sheath in the damaged area of 
      SCI + hucMSCs group were also significantly increased compared to the SCI + PBS 
      group. HucMSCs transplanting decreased the expression of protein level of 
      Caspase-3 and Bax and increased the Bcl-2, MBP and CNPase, rescued the apoptosis 
      of neurons and the oligodendrocyte. CONCLUSION: These results showed that hucMSCs 
      can improve motor function, tissue repairing and reducing apoptosis in SCI rats.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Liao, Ziling
AU  - Liao Z
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Yang, Xiuzhen
AU  - Yang X
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Deng, Weiyue
AU  - Deng W
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Zhang, Yuying
AU  - Zhang Y
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Song, Aishi
AU  - Song A
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China.
FAU - Ni, Bin
AU  - Ni B
AD  - NHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial 
      Maternal and Child Health Care Hospital, Changsha, Hunan 410008, China.
FAU - Zhao, Huifang
AU  - Zhao H
AD  - CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine 
      and Health and Regenerative Medicine, Guangzhou Institutes of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Zhang, Shusheng
AU  - Zhang S
AD  - Changsha Stomatological Hospital, Changsha, Hunan 410004, China.
FAU - Li, Zhiyuan
AU  - Li Z
AD  - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410013, China; NHC Key Laboratory of Birth Defect for 
      Research and Prevention, Hunan Provincial Maternal and Child Health Care 
      Hospital, Changsha, Hunan 410008, China; CAS Key Laboratory of Regenerative 
      Biology, Guangzhou Institutes of Biomedicine and Health and Regenerative 
      Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of 
      Sciences, Guangzhou 510530, China; GZMU-GIBH Joint School of Life Sciences, 
      Guangzhou Medical University, Guangdong, China; Changsha Stomatological Hospital, 
      Changsha, Hunan 410004, China; Guangzhou Regenerative Medicine and Health 
      Guangdong Laboratory, Guangzhou 510005, China. Electronic address: 
      li_zhiyuan@gibh.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20210115
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Myelin Basic Protein)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Blood-Brain Barrier/pathology
MH  - Demyelinating Diseases/*prevention & control
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - *Locomotion
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Myelin Basic Protein/metabolism
MH  - Neurons/pathology
MH  - Oligodendroglia/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Spinal Cord Injuries/*pathology/psychology/*therapy
OTO - NOTNLM
OT  - Apoptosis
OT  - Myelin sheath
OT  - Neurons
OT  - Spinal cord injury
OT  - hucMSCs
EDAT- 2021/01/25 06:00
MHDA- 2021/11/16 06:00
CRDT- 2021/01/24 20:39
PHST- 2020/10/13 00:00 [received]
PHST- 2020/12/18 00:00 [revised]
PHST- 2021/01/10 00:00 [accepted]
PHST- 2021/01/25 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/01/24 20:39 [entrez]
AID - S0143-4179(21)00011-1 [pii]
AID - 10.1016/j.npep.2021.102125 [doi]
PST - ppublish
SO  - Neuropeptides. 2021 Apr;86:102125. doi: 10.1016/j.npep.2021.102125. Epub 2021 Jan 
      15.