PMID- 33488110
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220420
IS  - 1178-7031 (Print)
IS  - 1178-7031 (Electronic)
IS  - 1178-7031 (Linking)
VI  - 14
DP  - 2021
TI  - Selenium-Containing Amino Acids Protect Dextran Sulfate Sodium-Induced Colitis 
      via Ameliorating Oxidative Stress and Intestinal Inflammation.
PG  - 85-95
LID - 10.2147/JIR.S288412 [doi]
AB  - BACKGROUND: Inflammatory bowel disease (IBD) is characterized by chronic 
      relapsing inflammation of the gastrointestinal tract. Oxidative stress plays a 
      pivotal role in the pathogenesis of IBD. Selenium-containing amino acids 
      reportedly have anti-oxidative and anti-inflammatory properties, but it remains 
      unknown if selenium-containing amino acids can be used to treat IBD. This study 
      aimed to investigate the effects of two selenium-containing amino acids - 
      selenocysteine and selenocystine - on oxidative stress and chronic inflammation 
      in a mouse model of dextran sulfate sodium (DSS)-induced IBD. METHODOLOGY: 
      C57BL/6 mice were randomly assigned to the following six groups: control, DSS, 
      DSS+selenocysteine, DSS+selenocystine, DSS+sodium selenite, and 
      DSS+N-acetylcysteine (NAC). IBD was induced by 3% DSS. Pro-inflammatory cytokines 
      [interleukin-1beta (IL-1beta), monocyte chemotactic protein 1 (MCP-1), IL-6, and tumor 
      necrosis factor-alpha (TNF-alpha)] and markers for oxidative and anti-oxidative stress 
      [malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase 
      (SOD), and glutathione peroxidase (GPx)] were measured using immunohistochemical 
      analysis. RESULTS: Selenocysteine and selenocystine significantly attenuated 
      IBD-related symptoms, including preventing weight loss, decreasing disease 
      activity index (DAI) scores, and increasing colon length. Selenocysteine and 
      selenocystine significantly ameliorated the DSS-induced oxidative stress, as 
      demonstrated by a reduction in ROS and MDA activity and an increase in SOD and 
      GPx activity. IL-1, MCP-1, IL-6, and TNF-alpha levels were significantly increased in 
      the IBD mice, while treatment with the selenium-containing amino acids 
      significantly reduced the levels of these pro-inflammatory cytokines. In vivo 
      safety analysis showed minimal side effects of the selenium-containing amino 
      acids. CONCLUSION: We found that selenocysteine and selenocystine ameliorated 
      DSS-induced IBD via reducing oxidative stress and intestinal inflammation, 
      indicating that selenium-containing amino acids could be a novel therapeutic 
      option for patients with IBD.
CI  - (c) 2021 Shi et al.
FAU - Shi, Chengxin
AU  - Shi C
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Yue, Fengli
AU  - Yue F
AD  - College of Basic Medical Sciences, Jilin University, Changchun 130021, People's 
      Republic of China.
FAU - Shi, Feiyu
AU  - Shi F
AUID- ORCID: 0000-0001-5691-1362
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Qin, Qian
AU  - Qin Q
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Wang, Lizhao
AU  - Wang L
AUID- ORCID: 0000-0002-7782-322X
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Wang, Guanghui
AU  - Wang G
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Mu, Lijun
AU  - Mu L
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Liu, Dan
AU  - Liu D
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Li, Yaguang
AU  - Li Y
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - Yu, Tianyu
AU  - Yu T
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
FAU - She, Junjun
AU  - She J
AUID- ORCID: 0000-0002-0853-4280
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210114
PL  - New Zealand
TA  - J Inflamm Res
JT  - Journal of inflammation research
JID - 101512684
PMC - PMC7814278
OTO - NOTNLM
OT  - Inflammatory bowel diseases
OT  - colitis
OT  - inflammation
OT  - oxidative stress
OT  - selenium-containing amino acids
OT  - selenocysteine
OT  - selenocystine
COIS- The authors declare that there is no conflict of interest.
EDAT- 2021/01/26 06:00
MHDA- 2021/01/26 06:01
PMCR- 2021/01/14
CRDT- 2021/01/25 05:37
PHST- 2020/10/23 00:00 [received]
PHST- 2020/12/31 00:00 [accepted]
PHST- 2021/01/25 05:37 [entrez]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/01/26 06:01 [medline]
PHST- 2021/01/14 00:00 [pmc-release]
AID - 288412 [pii]
AID - 10.2147/JIR.S288412 [doi]
PST - epublish
SO  - J Inflamm Res. 2021 Jan 14;14:85-95. doi: 10.2147/JIR.S288412. eCollection 2021.