PMID- 33488296
OWN - NLM
STAT- MEDLINE
DCOM- 20211103
LR  - 20231110
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2021
DP  - 2021
TI  - MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with 
      Endotoxemia in Equine Colic.
PG  - 9501478
LID - 10.1155/2021/9501478 [doi]
LID - 9501478
AB  - The purpose of the study was to compare the results of sepsis scoring (clinical 
      examination and clinical pathology) to the concentrations of 
      matrix-metalloproteinases (MMPs) -2, -8, and -9; tissue-inhibitor of 
      metalloproteinases (TIMPs) -1 and -2; and inflammatory chemokines interleukin 
      (IL) 1beta and tumor-necrosis-factor-alpha (TNF-alpha) in plasma and peritoneal fluid of 
      equine colic patients. A modified sepsis scoring including general condition, 
      heart and respiratory rate, rectal temperature, mucous membranes, white blood 
      cell count (WBC), and ionized calcium was applied in 47 horses presented with 
      clinical signs of colic. Using this scoring system, horses were classified as 
      negative (n = 32, </=6/19 points), questionable (n = 9, 7-9/19 points), or positive 
      (n = 6, >/=10/19 points) for sepsis. MMPs, TIMPs, IL-1beta, and TNF-alpha concentrations 
      were evaluated in plasma and peritoneal fluid using species-specific sandwich 
      ELISA kits. In a linear discriminant analysis, all parameters of sepsis scoring 
      apart from calcium separated well between sepsis severity groups (P < 0.05). 
      MMP-9 was the only biomarker of high diagnostic value, while all others remained 
      insignificant. A significant influence of overall sepsis scoring on MMP-9 was 
      found for peritoneal fluid (P = 0.005) with a regression coefficient of 0.092, 
      while no association was found for plasma (P = 0.085). Using a MMP-9 
      concentration of >113 ng/ml in the peritoneal fluid was found to be the ideal 
      cutoff to identify positive sepsis scoring (>/=10/19 points; sensitivity of 83.3% 
      and specificity of 82.9%). In conclusion, MMP-9 was found to be a biomarker of 
      high diagnostic value for sepsis and endotoxemia in equine colic. The evaluation 
      of peritoneal fluid seems preferable in comparison to plasma. As abdominocentesis 
      is commonly performed in the diagnostic work-up of equine colic, a pen-side assay 
      would be useful and easy-to-perform diagnostic support in the decision for 
      therapeutic intervention.
CI  - Copyright (c) 2021 Ann Kristin Barton et al.
FAU - Barton, Ann Kristin
AU  - Barton AK
AUID- ORCID: 0000-0002-3333-9647
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
FAU - Richter, Ina-Gabriele
AU  - Richter IG
AD  - Research Centre of Medical Technology and Biotechnology, Bad Langensalza, 
      Germany.
FAU - Ahrens, Tanja
AU  - Ahrens T
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
FAU - Merle, Roswitha
AU  - Merle R
AUID- ORCID: 0000-0002-8688-2926
AD  - Institute for Veterinary Epidemiology, Freie Universitaet Berlin, Berlin, 
      Germany.
FAU - Alalwani, Abdollah
AU  - Alalwani A
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
FAU - Lilge, Svenja
AU  - Lilge S
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
FAU - Purschke, Katrin
AU  - Purschke K
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
FAU - Barnewitz, Dirk
AU  - Barnewitz D
AD  - Research Centre of Medical Technology and Biotechnology, Bad Langensalza, 
      Germany.
FAU - Gehlen, Heidrun
AU  - Gehlen H
AUID- ORCID: 0000-0001-5451-4232
AD  - Equine Clinic, Freie Universitaet Berlin, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20210105
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Biomarkers)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.34 (Matrix Metalloproteinase 8)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/*chemistry
MH  - Biomarkers/*metabolism
MH  - Colic/*metabolism
MH  - Endotoxemia/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Horses
MH  - Interleukin-1beta/metabolism
MH  - Leukocyte Count
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 8/metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Sepsis/blood
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
MH  - Tumor Necrosis Factor-alpha
PMC - PMC7803393
COIS- The authors declare that none of them have conflicts of interest.
EDAT- 2021/01/26 06:00
MHDA- 2021/11/04 06:00
PMCR- 2021/01/05
CRDT- 2021/01/25 05:38
PHST- 2020/07/20 00:00 [received]
PHST- 2020/12/03 00:00 [revised]
PHST- 2020/12/28 00:00 [accepted]
PHST- 2021/01/25 05:38 [entrez]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/11/04 06:00 [medline]
PHST- 2021/01/05 00:00 [pmc-release]
AID - 10.1155/2021/9501478 [doi]
PST - epublish
SO  - Mediators Inflamm. 2021 Jan 5;2021:9501478. doi: 10.1155/2021/9501478. 
      eCollection 2021.