PMID- 33489038
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220420
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 23
IP  - 12
DP  - 2020 Dec
TI  - Bee venom attenuates neurodegeneration and motor impairment and modulates the 
      response to L-dopa or rasagiline in a mice model of Parkinson's disease.
PG  - 1628-1638
LID - 10.22038/ijbms.2020.46469.10731 [doi]
AB  - OBJECTIVES: This study aimed to investigate the effect of bee venom, a form of 
      alternative therapy, on rotenone-induced Parkinson's disease (PD) in mice. 
      Moreover, the possible modulation by bee venom of the effect of L-dopa/carbidopa 
      or rasagiline was examined. MATERIALS AND METHODS: Rotenone (1.5 mg/kg, 
      subcutaneously; SC) was administered every other day for two weeks and at the 
      same time mice received the vehicle (DMSO, SC), bee venom (0.065, 0.13, and 0.26 
      mg/kg; intradermal; ID), L-dopa/carbidopa (25 mg/kg, intraperitoneal; IP), 
      L-dopa/carbidopa+bee venom (0.13 mg/kg, ID), rasagiline (1 mg/kg, IP) or 
      rasagiline+bee venom (0.13 mg/kg, ID). Then, wire hanging and staircase tests 
      were performed and mice were euthanized and brains' striata separated. Oxidative 
      stress biomarkers namely, malondialdehyde (MDA), nitric oxide (NO), reduced 
      glutathione (GSH), paraoxonase-1 (PON-1), and total antioxidant capacity (TAC) 
      were measured. Additionally, butyrylcholinesterase (BuChE), monocyte 
      chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha), and 
      dopamine (DA) were evaluated. Brain histopathological changes and caspase-3- 
      expression were done. RESULTS: Bee venom significantly enhanced motor performance 
      and inhibited rotenone-induced oxidative/nitrosative stress, observed as a 
      reduction in both MDA and NO along with increasing GSH, PON-1, and TAC. Besides, 
      bee venom decreased MCP-1, TNF-alpha, and caspase-3 expression together with an 
      increase in BuChE activity and DA content. CONCLUSION: Bee venom alone or in 
      combination with L-dopa/carbidopa or rasagiline alleviated neuronal degeneration 
      compared with L-dopa/carbidopa or rasagiline treatment only. Bee venom via its 
      antioxidant and cytokine reducing potentials might be of value either alone or as 
      adjunctive therapy in the management of PD.
FAU - Badawi, Hanaa Mm
AU  - Badawi HM
AD  - Holding Company for Biological Products, Vaccines and Drugs (VACSERA), Cairo, 
      Egypt.
FAU - Abdelsalam, Rania M
AU  - Abdelsalam RM
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, 
      Cairo, Egypt.
FAU - Abdel-Salam, Omar Me
AU  - Abdel-Salam OM
AD  - Department of Toxicology and Narcotics, National Research Centre, Cairo, Egypt.
FAU - Youness, Eman R
AU  - Youness ER
AD  - Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.
FAU - Shaffie, Nermeen M
AU  - Shaffie NM
AD  - Department of Pathology, National Research Centre, Cairo, Egypt.
FAU - Eldenshary, Ezz-El Din S
AU  - Eldenshary EDS
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, 
      Cairo, Egypt.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC7811814
OTO - NOTNLM
OT  - Bee venom
OT  - Dopamine
OT  - Oxidative stress
OT  - Parkinson's disease
OT  - Rotenone
EDAT- 2021/01/26 06:00
MHDA- 2021/01/26 06:01
PMCR- 2020/12/01
CRDT- 2021/01/25 05:40
PHST- 2021/01/25 05:40 [entrez]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/01/26 06:01 [medline]
PHST- 2020/12/01 00:00 [pmc-release]
AID - 10.22038/ijbms.2020.46469.10731 [doi]
PST - ppublish
SO  - Iran J Basic Med Sci. 2020 Dec;23(12):1628-1638. doi: 
      10.22038/ijbms.2020.46469.10731.