PMID- 33489476
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210126
IS  - 2164-7712 (Print)
IS  - 2164-7712 (Electronic)
VI  - 9
IP  - 5
DP  - 2020
TI  - Resveratrol attenuates stimulated T-cell activation and proliferation: potential 
      therapy against cellular rejection in organ transplantation.
PG  - 81-90
AB  - BACKGROUND: Pharmaceuticals to inhibit mammalian target of rapamycin (mTOR) 
      protein, which plays an integral role in T cell survival and function, have been 
      used to prevent complications associated with organ transplantation. Although 
      studies have individually shown that resveratrol can inhibit mTOR and that 
      inhibiting mTOR leads to attenuated immune function, no studies to date have 
      examined these two functions conjointly under one study. Therefore, we 
      hypothesize that resveratrol will decrease mTOR activation and expression as well 
      as attenuate stimulated T cell activation and proliferation in peripheral blood 
      mononuclear cells (PBMC). METHODS AND MATERIALS: Human PBMC were isolated and 
      cultured. The cells were pre-treated with resveratrol (50 muM) overnight (18 hrs) 
      before stimulation. The cells were collected for subsequent biochemical analysis 
      after 1, 3, and 5 days. Additionally, the cells were stained with proliferation 
      dye and cultured for 24 hours in PMA/Ionomycin with resveratrol for flow 
      cytometry analysis. RESULTS: Resveratrol treated stimulated PBMCs displayed a 
      significant decrease in activated phosphorylation of mTOR at days 1, 3, and 5 (P 
      < 0.0329). Markers of T cell activation, tumour necrosis factor-alpha (TNF-alpha) and 
      interferon-gamma (INF-gamma), were also significantly reduced along with T cell 
      proliferation following stimulated PBMC resveratrol treatment when compared to 
      vehicle-treated controls (P < 0.01). CONCLUSION: Taken together, our data suggest 
      that resveratrol can decrease the immune response of stimulated T-cells and 
      inhibit the expression and activation of mTOR mediated cellular signalling under 
      the same study setting. Therefore, resveratrol proposes a possible adjunctive 
      therapy option for patients undergoing organ transplantation.
CI  - AJCEI Copyright (c) 2020.
FAU - Kang, Jimmy Jh
AU  - Kang JJ
AD  - Faculty of Medicine and Dentistry, University of Alberta Edmonton, AB, Canada.
FAU - Bozso, Sabin J
AU  - Bozso SJ
AD  - Department of Surgery, Division of Cardiac Surgery, University of Alberta 
      Edmonton, AB, Canada.
FAU - Boe, Dana E
AU  - Boe DE
AD  - Faculty of Medicine and Dentistry, University of Alberta Edmonton, AB, Canada.
FAU - Al-Adra, David P
AU  - Al-Adra DP
AD  - Department of Surgery, Division of Transplantation, University of Wisconsin 
      School of Medicine and Public Health Madison, WI, USA.
FAU - Moon, Michael C
AU  - Moon MC
AD  - Department of Surgery, Division of Cardiac Surgery, University of Alberta 
      Edmonton, AB, Canada.
FAU - Freed, Darren H
AU  - Freed DH
AD  - Department of Surgery, Division of Cardiac Surgery, University of Alberta 
      Edmonton, AB, Canada.
FAU - Nagendran, Jayan
AU  - Nagendran J
AD  - Department of Surgery, Division of Cardiac Surgery, University of Alberta 
      Edmonton, AB, Canada.
FAU - Nagendran, Jeevan
AU  - Nagendran J
AD  - Department of Surgery, Division of Cardiac Surgery, University of Alberta 
      Edmonton, AB, Canada.
LA  - eng
PT  - Journal Article
DEP - 20201215
PL  - United States
TA  - Am J Clin Exp Immunol
JT  - American journal of clinical and experimental immunology
JID - 101587157
PMC - PMC7811925
OTO - NOTNLM
OT  - Resveratrol
OT  - lymphocytes
OT  - organ transplantation
COIS- None.
EDAT- 2021/01/26 06:00
MHDA- 2021/01/26 06:01
PMCR- 2020/12/15
CRDT- 2021/01/25 05:42
PHST- 2020/08/15 00:00 [received]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2021/01/25 05:42 [entrez]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/01/26 06:01 [medline]
PHST- 2020/12/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Clin Exp Immunol. 2020 Dec 15;9(5):81-90. eCollection 2020.