PMID- 33489859 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220102 IS - 1976-8257 (Print) IS - 2234-2753 (Electronic) IS - 1976-8257 (Linking) VI - 37 IP - 1 DP - 2021 Jan TI - Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo. PG - 71-83 LID - 10.1007/s43188-020-00064-z [doi] AB - The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 mug/ml, Elastin-1.5-fold increase at 1mug/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 mug/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 mug/ml) and heme oxygenase 1 (1.5-fold increase at 25 mug/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care. CI - (c) Korean Society of Toxicology 2020. FAU - Hong, Youngeun AU - Hong Y AD - Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 FAU - Lee, Hyunji AU - Lee H AD - Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 FAU - Tran, Quangdon AU - Tran Q AD - Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 FAU - Bayarmunkh, Choinyam AU - Bayarmunkh C AD - Department of Graduate Education, Graduate school, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia. GRID: grid.444534.6 AD - Department of Physiology, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia. GRID: grid.444534.6 FAU - Boldbaatar, Damdindorj AU - Boldbaatar D AD - Department of Graduate Education, Graduate school, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia. GRID: grid.444534.6 AD - Department of Physiology, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia. GRID: grid.444534.6 FAU - Kwon, So Hee AU - Kwon SH AD - College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, 21983 South Korea. GRID: grid.15444.30. ISNI: 0000 0004 0470 5454 FAU - Park, Jongsun AU - Park J AUID- ORCID: 0000-0002-4690-1854 AD - Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 FAU - Park, Jisoo AU - Park J AD - Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea. GRID: grid.254230.2. ISNI: 0000 0001 0722 6377 AD - Department of Life Science, Hyehwa Liberal Arts College, Daejeon University, Daejeon, 34520 South Korea. GRID: grid.411948.1. ISNI: 0000 0001 0523 5122 LA - eng PT - Journal Article DEP - 20201020 PL - Singapore TA - Toxicol Res JT - Toxicological research JID - 101483324 PMC - PMC7806687 OTO - NOTNLM OT - Anti-aging OT - Anti-oxidant OT - Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roos OT - Extracts OT - NHDF cells OT - Wound healing COIS- Conflict of InterestThe authors declare that they have no competing interests. EDAT- 2021/01/26 06:00 MHDA- 2021/01/26 06:01 PMCR- 2022/01/01 CRDT- 2021/01/25 05:44 PHST- 2020/07/03 00:00 [received] PHST- 2020/08/21 00:00 [revised] PHST- 2020/09/01 00:00 [accepted] PHST- 2021/01/25 05:44 [entrez] PHST- 2021/01/26 06:00 [pubmed] PHST- 2021/01/26 06:01 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 64 [pii] AID - 10.1007/s43188-020-00064-z [doi] PST - epublish SO - Toxicol Res. 2020 Oct 20;37(1):71-83. doi: 10.1007/s43188-020-00064-z. eCollection 2021 Jan.