PMID- 33490086
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220420
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 8
DP  - 2020
TI  - Circular RNA CircNOLC1, Upregulated by NF-KappaB, Promotes the Progression of 
      Prostate Cancer via miR-647/PAQR4 Axis.
PG  - 624764
LID - 10.3389/fcell.2020.624764 [doi]
LID - 624764
AB  - BACKGROUND: CircRNAs recently have shown critical roles in tumor biology. 
      However, their roles in prostate cancer (PCa) remains largely unclear. METHODS: 
      CircRNA microarrays were performed in immortal prostate cell line RWPE1 and PCa 
      cell lines as DU145, PC3, LNCaP, C4-2, and 22RV1. Combined with upregulated 
      circRNAs in PCa tissues, circNOLC1 expression was validated in PCa cells and 
      tissues via qRT-PCR and FISH. Sanger sequencing, actinomycin D, gDNA, and cDNA, 
      RNase R assays were used to assess the circular characteristics of circNOLC1. 
      CCK-8, colony formation, transwell migration assays, and mice xenograft models 
      were conducted to evaluate the functions of PCa cells after circNOLC1 knockdown 
      and overexpression. RNA pulldown, luciferase reporter assay, FISH (fluorescence 
      in situ hybridization), and CHIP were utilized to illustrate the further 
      mechanisms of circNOLC1. RESULTS: Our research indicated that circNOLC1 was 
      overexpressed in PCa cells and tissues, and circNOLC1 was more stable than linear 
      NOLC1 mRNA. CircNOLC1 promoted PCa cells proliferation and migration in vitro and 
      vivo. Additionally, we found that circNOLC1 could upregulate PAQR4 expression by 
      sponging miR-647, leading to the activation of PI3K/Akt pathway. Moreover, 
      NF-kappaB was identified to bind to the NOLC1 promoter sites and upregulated both 
      NOLC1 and circNOLC1 expression. CONCLUSION: CircNOLC1, elevated by transcription 
      factor NF-kappaB, promotes PCa progression via a miR-647/PAQR4 axis, and 
      circNOLC1 is a potential biomarker and target for PCa treatment.
CI  - Copyright (c) 2021 Chen, Cen, Zhou, Yang, Wu, Zou, Luo, Li, Lv and Mao.
FAU - Chen, Wenbin
AU  - Chen W
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Cen, Shengren
AU  - Cen S
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Zhou, Xumin
AU  - Zhou X
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Yang, Taowei
AU  - Yang T
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Wu, Kaihui
AU  - Wu K
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Zou, Libin
AU  - Zou L
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Luo, Junqi
AU  - Luo J
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Li, Chuanyin
AU  - Li C
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
FAU - Lv, Daojun
AU  - Lv D
AD  - Guangdong Key Laboratory of Urology, Department of Urology, The First Affiliated 
      Hospital of Guangzhou Medical University, Guangzhou, China.
FAU - Mao, Xiangming
AU  - Mao X
AD  - Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210108
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7820754
OTO - NOTNLM
OT  - NF-kappaB
OT  - PAQR4
OT  - circRNAs
OT  - miR-647
OT  - progression
OT  - prostate cancer
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/01/26 06:00
MHDA- 2021/01/26 06:01
PMCR- 2020/01/01
CRDT- 2021/01/25 05:44
PHST- 2020/11/01 00:00 [received]
PHST- 2020/12/14 00:00 [accepted]
PHST- 2021/01/25 05:44 [entrez]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/01/26 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2020.624764 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2021 Jan 8;8:624764. doi: 10.3389/fcell.2020.624764. 
      eCollection 2020.