PMID- 33493944
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 200
DP  - 2021 Apr
TI  - Effect of surface modification on physical and cellular properties of PCL thin 
      film.
PG  - 111582
LID - S0927-7765(21)00026-6 [pii]
LID - 10.1016/j.colsurfb.2021.111582 [doi]
AB  - Lack of suitable surface functional groups is one of the main limitations related 
      to the cell attachment of Polycaprolactone (PCL). The aim of this research was to 
      surface modify the PCL film using gelatin coating, via a simple physical 
      entrapment process. In this regard, after preparation of PCL films using casting, 
      they were immersed in each gelatin solutions. Consequently, chemical crosslinking 
      using glutaraldehyde was performed to improve the stability of the PCL-gelatin 
      film. Attenuated total reflectance-Fourier transform infrared spectroscopy 
      (ATR-FTIR), Scanning electron microscope (SEM), contact angle measurement, strip 
      tensile test, Dimethylthiazol-diphenyltetrazolium bromide (MTT) assay and Cell 
      seeding were used to evaluate the quality of the coating layer, the thickness of 
      PCL-gelatin film, the surface wettability, their mechanical properties, Cell 
      viability and Cell attachment and proliferation respectively. Results showed that 
      the amount of entrapped gelatin enhanced with increasing acetone in the gelatin 
      solution. Surface modification led to a two-fold increment of mechanical 
      strength, about 50% increase in elastic modulus, 54% in elongation and up to 11% 
      increment in cell viability. Moreover, wettability and cell attachment of PCL 
      film significantly enhanced, after gelatin modification. In conclusion, the 
      simple and cost effective modification of PCL using gelatin entrapment could 
      provide significant mechanical and biological properties making it a promising 
      approach for development of three-dimensional scaffolds for bone tissue 
      engineering.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Khorramnezhad, Mojtaba
AU  - Khorramnezhad M
AD  - Department of Life Science Engineering, Faculty of New Sciences and Technologies, 
      University of Tehran, Tehran, Iran.
FAU - Akbari, Babak
AU  - Akbari B
AD  - Department of Life Science Engineering, Faculty of New Sciences and Technologies, 
      University of Tehran, Tehran, Iran. Electronic address: babakbari@ut.ac.ir.
FAU - Akbari, Mahdokht
AU  - Akbari M
AD  - Department of Life Science Engineering, Faculty of New Sciences and Technologies, 
      University of Tehran, Tehran, Iran.
FAU - Kharaziha, Mahshid
AU  - Kharaziha M
AD  - Department of Materials Engineering, Isfahan University of Technology, Isfahan, 
      84156-83111, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210116
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Polyesters)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Bone and Bones
MH  - Elastic Modulus
MH  - *Gelatin
MH  - *Polyesters
MH  - Surface Properties
MH  - Tissue Engineering
MH  - Tissue Scaffolds
MH  - Wettability
OTO - NOTNLM
OT  - Gelatin
OT  - Physical entrapment
OT  - Polycaprolactone
OT  - Surface modification
OT  - Thin film
EDAT- 2021/01/26 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/01/25 20:15
PHST- 2019/12/24 00:00 [received]
PHST- 2020/12/26 00:00 [revised]
PHST- 2021/01/13 00:00 [accepted]
PHST- 2021/01/26 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/01/25 20:15 [entrez]
AID - S0927-7765(21)00026-6 [pii]
AID - 10.1016/j.colsurfb.2021.111582 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2021 Apr;200:111582. doi: 
      10.1016/j.colsurfb.2021.111582. Epub 2021 Jan 16.