PMID- 33494207 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210316 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 10 IP - 2 DP - 2021 Jan 21 TI - Influence of Refrigerated Storage on Water Status, Protein Oxidation, Microstructure, and Physicochemical Qualities of Atlantic Mackerel (Scomber scombrus). LID - 10.3390/foods10020214 [doi] LID - 214 AB - Moisture migration, protein oxidation, microstructure, and the physicochemical qualities of Atlantic mackerel during storage at 4 degrees C and 0 degrees C were explored in this study. Three proton components were observed in mackerel muscle using low-field nuclear magnetic resonance relaxation, which were characterized as bound water, immobilized water, and lipid. The relaxation peak of immobilized water shifted to a shorter relaxation time and its intensity decreased with the proceeding of the storage process. T(1) and T(2) weighted images obtained by magnetic resonance imaging showed a slightly continuous decrease in the intensity of water. The significant decrease in sulfhydryl (SH) content and the increase in carbonyl group (CP) content, disulfide bond content, and hydrophobicity revealed the oxidation of protein during storage. The contents of alpha-helixes in proteins decreased while that of random coils increased during storage, which suggested changes in the secondary structure of mackerel protein. The storage process also caused the contraction and fracture of myofibrils, and the granulation of endolysin protein. In addition, the drip loss, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS) value, and b* value increased significantly with the storage time. FAU - Lin, Rong AU - Lin R AD - National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China. AD - National Engineering Research Center of Seafood, Dalian 116034, China. FAU - Cheng, Shasha AU - Cheng S AD - National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China. AD - National Engineering Research Center of Seafood, Dalian 116034, China. FAU - Wang, Siqi AU - Wang S AD - National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China. AD - National Engineering Research Center of Seafood, Dalian 116034, China. FAU - Tan, Mingqian AU - Tan M AUID- ORCID: 0000-0002-7535-0035 AD - National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China. AD - National Engineering Research Center of Seafood, Dalian 116034, China. FAU - Zhu, Beiwei AU - Zhu B AD - National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China. AD - National Engineering Research Center of Seafood, Dalian 116034, China. LA - eng GR - 31972105/National Natural Science Foundation of China/ GR - 2020JH6/10500002/Central Funds Guiding the Local Science and Technology Development of China/ PT - Journal Article DEP - 20210121 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC7909831 OTO - NOTNLM OT - Atlantic mackerel OT - microstructure OT - secondary structure of protein OT - water status and distribution COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2021/01/27 06:00 MHDA- 2021/01/27 06:01 PMCR- 2021/01/21 CRDT- 2021/01/26 01:00 PHST- 2020/12/27 00:00 [received] PHST- 2021/01/15 00:00 [revised] PHST- 2021/01/19 00:00 [accepted] PHST- 2021/01/26 01:00 [entrez] PHST- 2021/01/27 06:00 [pubmed] PHST- 2021/01/27 06:01 [medline] PHST- 2021/01/21 00:00 [pmc-release] AID - foods10020214 [pii] AID - foods-10-00214 [pii] AID - 10.3390/foods10020214 [doi] PST - epublish SO - Foods. 2021 Jan 21;10(2):214. doi: 10.3390/foods10020214.