PMID- 33497178
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 7
IP  - 2
DP  - 2021 Feb 8
TI  - Cellular Response to Sol-Gel Hybrid Materials Releasing Boron and Calcium Ions.
PG  - 491-506
LID - 10.1021/acsbiomaterials.0c01546 [doi]
AB  - Poly(dimethylsiloxane) (PDMS)-SiO(2)-CaO-based hybrid materials prepared by 
      sol-gel have proved to be very promising materials for tissue engineering 
      applications and drug-delivery systems. These hybrids are biocompatible and 
      present osteogenic and bioactive properties supporting osteoblast attachment and 
      bone growth. The incorporation of therapeutic elements in these materials, such 
      as boron (B) and calcium (Ca), was considered in this study as an approach to 
      develop biomaterials capable of stimulating bone regeneration. The main purpose 
      of this work was thus to produce, by sol-gel, bioactive and biocompatible hybrid 
      materials of the PDMS-SiO(2)-B(2)O(3)-CaO system, capable of a controlled Ca and 
      B release. Different compositions with different boron amounts were prepared 
      using the same precursors resulting in different monolithic materials, with 
      distinct structures and microstructures. Structural features were assessed by 
      Fourier transform infrared (FT-IR) spectrometry and solid-state nuclear magnetic 
      resonance (NMR) techniques, which confirmed the presence of hybrid bonds 
      (Si-O-Si) between organic (PDMS) and inorganic phase (tetraethyl orthosilicate 
      (TEOS)), as well as borosiloxane bonds (B-O-Si). From the (11)B NMR results, it 
      was found that Ca changes the boron coordination, from trigonal (BO(3)) to 
      tetrahedral (BO(4)). Scanning electron microscopy (SEM) micrographs and N(2) 
      isotherms showed that the incorporation of boron modifies the material's 
      microstructure by increasing the macroporosity and decreasing the specific 
      surface area (SSA). In vitro tests in simulated body fluid (SBF) showed the 
      precipitation of a calcium phosphate layer on the material surface and the 
      controlled release of therapeutic ions. The cytocompatibility of the prepared 
      hybrids was studied with bone marrow stromal cells (ST-2 cell line) by analyzing 
      the cell viability and cell density. The results demonstrated that increasing the 
      dilution rate of extraction medium from the hybrids leads to improved cell 
      behavior. The relationship between the in vitro response and the structural and 
      microstructural features of the materials was explored. It was shown that the 
      release of calcium and boron ions, determined by the hybrid structure was crucial 
      for the observed cells behavior. Although not completely understood, the 
      encouraging results obtained constitute an incentive for further studies on this 
      topic.
FAU - Coelho, Soraia Alexandra Ramos
AU  - Coelho SAR
AD  - Department of Materials and Ceramic Engineering, CICECO-Aveiro Institute of 
      Materials (CICECO/UA), University of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Almeida, Jose Carlos
AU  - Almeida JC
AUID- ORCID: 0000-0003-1552-9532
AD  - Department of Materials and Ceramic Engineering, CICECO-Aveiro Institute of 
      Materials (CICECO/UA), University of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Unalan, Irem
AU  - Unalan I
AD  - Institute of Biomaterials, Department of Materials Science and Engineering, 
      Friedrich-Alexander-University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 
      Erlangen, Germany.
FAU - Detsch, Rainer
AU  - Detsch R
AD  - Institute of Biomaterials, Department of Materials Science and Engineering, 
      Friedrich-Alexander-University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 
      Erlangen, Germany.
FAU - Miranda Salvado, Isabel Margarida
AU  - Miranda Salvado IM
AD  - Department of Materials and Ceramic Engineering, CICECO-Aveiro Institute of 
      Materials (CICECO/UA), University of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Boccaccini, Aldo R
AU  - Boccaccini AR
AUID- ORCID: 0000-0002-7377-2955
AD  - Institute of Biomaterials, Department of Materials Science and Engineering, 
      Friedrich-Alexander-University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 
      Erlangen, Germany.
FAU - Fernandes, Maria Helena Vaz
AU  - Fernandes MHV
AUID- ORCID: 0000-0002-1240-2512
AD  - Department of Materials and Ceramic Engineering, CICECO-Aveiro Institute of 
      Materials (CICECO/UA), University of Aveiro, 3810-193 Aveiro, Portugal.
AD  - The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at 
      University of Minho, Avepark, Barco, 4805-017 Guimaraes, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210126
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ions)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - N9E3X5056Q (Boron)
SB  - IM
MH  - Biocompatible Materials
MH  - *Boron
MH  - Ions
MH  - *Silicon Dioxide
MH  - Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - bioactivity
OT  - boron
OT  - calcium
OT  - cytocompatibility
OT  - hybrids
OT  - sol-gel
OT  - structure
EDAT- 2021/01/27 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/01/26 17:09
PHST- 2021/01/27 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/01/26 17:09 [entrez]
AID - 10.1021/acsbiomaterials.0c01546 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2021 Feb 8;7(2):491-506. doi: 
      10.1021/acsbiomaterials.0c01546. Epub 2021 Jan 26.