PMID- 33497195
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 93
IP  - 5
DP  - 2021 Feb 9
TI  - Structural Characterization of Isomeric Oligogalacturonan Mixtures Using 
      Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass 
      Spectrometry.
PG  - 2838-2847
LID - 10.1021/acs.analchem.0c04142 [doi]
AB  - Pectins are natural polysaccharides made from galacturonic acid residues, and 
      they are widely used as an excipient in food and pharmaceutical industries. The 
      degree of methyl-esterification, the monomeric composition, and the linkage 
      pattern are all important factors that influence the physical and chemical 
      properties of pectins, such as the solubility. This work focuses on the 
      successful online coupling of charge transfer dissociation-mass spectrometry 
      (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to 
      differentiate isomers of oligogalacturonans derived from citrus pectins. This 
      work employed CTD fragmentation of the pectin mixtures in data-dependent 
      acquisition mode. Compared to the UHPLC with collision-induced dissociation mass 
      spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more 
      structurally informative results. The developed UHPLC-CTD-MS method resulted in 
      abundant cross-ring cleavages-and especially (1,4)X(n), (1,5)X(n), and (2,4)X(n) 
      ions-which helped to identify most of the isomers. The Gal A isomers differed 
      only in the methyl group position along the galacturonic acid backbone. The 
      combination of CTD in real time with UHPLC provides a new tool for the structural 
      characterization of complex mixtures of oligogalacturonans and potentially other 
      classes of oligosaccharides.
FAU - Mendis, Praneeth M
AU  - Mendis PM
AD  - C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, 
      West Virginia 26506-6121, United States.
FAU - Sasiene, Zachary J
AU  - Sasiene ZJ
AD  - C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, 
      West Virginia 26506-6121, United States.
FAU - Ropartz, David
AU  - Ropartz D
AUID- ORCID: 0000-0003-4767-6940
AD  - INRAE, UR BIA, Nantes F-44316, France.
AD  - INRAE, BIBS Facility, Nantes F-44316, France.
FAU - Rogniaux, Helene
AU  - Rogniaux H
AUID- ORCID: 0000-0001-6083-2034
AD  - INRAE, UR BIA, Nantes F-44316, France.
AD  - INRAE, BIBS Facility, Nantes F-44316, France.
FAU - Jackson, Glen P
AU  - Jackson GP
AUID- ORCID: 0000-0003-0803-6254
AD  - C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, 
      West Virginia 26506-6121, United States.
AD  - Department of Forensic and Investigative Science, West Virginia University, 
      Morgantown, West Virginia 26506-6121, United States.
LA  - eng
GR  - R01 GM114494/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20210126
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Isomerism
MH  - Mass Spectrometry
MH  - *Oligosaccharides
MH  - *Polysaccharides
EDAT- 2021/01/27 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/01/26 17:09
PHST- 2021/01/27 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/01/26 17:09 [entrez]
AID - 10.1021/acs.analchem.0c04142 [doi]
PST - ppublish
SO  - Anal Chem. 2021 Feb 9;93(5):2838-2847. doi: 10.1021/acs.analchem.0c04142. Epub 
      2021 Jan 26.