PMID- 33497585 OWN - NLM STAT- MEDLINE DCOM- 20220221 LR - 20220221 IS - 1557-9042 (Electronic) IS - 0897-7151 (Linking) VI - 38 IP - 14 DP - 2021 Jul 15 TI - Sequential Expression of Chemokines in Chronic Subdural Hematoma Fluids after Trepanation Surgery. PG - 1979-1987 LID - 10.1089/neu.2020.7401 [doi] AB - Chronic subdural hematoma (CSDH) is considered an angiogenic and inflammatory disease. Chemokines attract leukocytes, and invading neutrophils and monocytes/macrophages play important roles in wound healing. However, no studies have been reported regarding changes in expression of chemokines in CSDH fluid after trepanation surgery. We randomly divided patients who underwent trepanation surgery into two groups. One was the irrigation group, in which irrigation of CSDH fluids was performed and a drainage tube was placed (n = 10). The other was the non-irrigation group, in which a drainage tube was inserted without irrigation (n = 10). CSDH fluids were collected during the trepanation surgery, immediately after surgery and on day 1 through the drainage tube. The concentrations of interleukin-8 (IL-8), growth-regulated oncogene-alpha (GRO-alpha), epithelial neutrophil-activating peptide 78 (ENA-78), monocyte chemoattractant protein-1 (MCP-1), interferon-gamma-induced protein-10 (IP-10), tissue plasminogen activator (tPA), von Willebrand factor (vWF), eotaxin-3, and myeloperoxidase (MPO) in each CSDH fluid sample were measured using enzyme-linked immunosorbent assay kits. After irrigation, concentrations of all chemokines decreased. However, concentrations of IL-8, GRO-alpha, ENA-78, MCP-1, and MPO were significantly increased on day 1 compared with concentrations during surgery with or without irrigation. In contrast, there were no changes in concentrations of IP-10, eotaxin-3, tPA, or vWF after trepanation surgery. Moreover, there were significant relationships among concentrations of IL-8, GRO-alpha, ENA-78, and MCP-1 during the surgery and on day 1. In CSDH fluids, chemokines that attract neutrophils, such as IL-8, GRO-alpha, ENA-78, and macrophage-attracting MCP-1, appear first after trepanation surgery, whereas lymphocyte-attracting IP-10 and eosinophil-attracting eotaxin-3 levels do not change within 1 day of surgery. These findings suggest that neutrophils and macrophages may play important roles in the healing process of CSDH at an early stage. FAU - Osuka, Koji AU - Osuka K AD - Department of Neurological Surgery, Aichi Medical University, Aichi, Japan. FAU - Ohmichi, Yusuke AU - Ohmichi Y AD - Department of Anatomy II, Kanazawa Medical University, Ishikawa, Japan. FAU - Ohmichi, Mika AU - Ohmichi M AD - Department of Anatomy II, Kanazawa Medical University, Ishikawa, Japan. FAU - Nakura, Takahiro AU - Nakura T AD - Department of Anatomy II, Kanazawa Medical University, Ishikawa, Japan. FAU - Iwami, Kenichiro AU - Iwami K AD - Department of Neurological Surgery, Aichi Medical University, Aichi, Japan. FAU - Watanabe, Yasuo AU - Watanabe Y AD - High Technology Research Center, Pharmacology, Showa Pharmaceutical University, Tokyo, Japan. FAU - Miyachi, Shigeru AU - Miyachi S AD - Department of Neurological Surgery, Aichi Medical University, Aichi, Japan. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20210127 PL - United States TA - J Neurotrauma JT - Journal of neurotrauma JID - 8811626 RN - 0 (Chemokines) SB - IM MH - Aged MH - Aged, 80 and over MH - Chemokines/*metabolism MH - Cohort Studies MH - Drainage MH - Female MH - Hematoma, Subdural, Chronic/*metabolism/*surgery MH - Humans MH - Male MH - Middle Aged MH - Therapeutic Irrigation MH - Time Factors MH - *Trephining OTO - NOTNLM OT - ENA-78 OT - GRO-alpha OT - MCP-1 OT - chronic subdural hematoma OT - interleukin-8 EDAT- 2021/01/27 06:00 MHDA- 2022/02/22 06:00 CRDT- 2021/01/26 20:08 PHST- 2021/01/27 06:00 [pubmed] PHST- 2022/02/22 06:00 [medline] PHST- 2021/01/26 20:08 [entrez] AID - 10.1089/neu.2020.7401 [doi] PST - ppublish SO - J Neurotrauma. 2021 Jul 15;38(14):1979-1987. doi: 10.1089/neu.2020.7401. Epub 2021 Jan 27.