PMID- 33498583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 14
IP  - 3
DP  - 2021 Jan 20
TI  - Development of pH-Responsive Polymer Coating as an Alternative to Enzyme-Based 
      Stem Cell Dissociation for Cell Therapy.
LID - 10.3390/ma14030491 [doi]
LID - 491
AB  - Cell therapy usually accompanies cell detachment as an essential process in cell 
      culture and cell collection for transplantation. However, conventional methods 
      based on enzymatic cell detachment can cause cellular damage including cell death 
      and senescence during the routine cell detaching step due to an inappropriate 
      handing. The aim of the current study is to apply the pH-responsive degradation 
      property of poly (amino ester) to the surface of a cell culture dish to provide a 
      simple and easy alternative method for cell detachment that can substitute the 
      conventional enzyme treatment. In this study, poly (amino ester) was modified 
      (cell detachable polymer, CDP) to show appropriate pH-responsive degradation 
      under mild acidic conditions (0.05% (w/v) CDP, pH 6.0) to detach stem cells 
      (human adipose tissue-derived stem cells (hADSCs)) perfectly within a short 
      period (less than 10 min). Compared to conventional enzymatic cell detachment, 
      hADSCs cultured on and detached from a CDP-coated cell culture dish showed 
      similar cellular properties. We further performed in vivo experiments on a mouse 
      hindlimb ischemia model (1.0 x 10(6) cells per limb). The in vivo results 
      indicated that hADSCs retrieved from normal cell culture dishes and CDP-coated 
      cell culture dishes showed analogous therapeutic angiogenesis. In conclusion, CDP 
      could be applied to a pH-responsive cell detachment system as a simple and easy 
      nonenzymatic method for stem cell culture and various cell therapies.
FAU - Kim, Yu-Jin
AU  - Kim YJ
AD  - School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
FAU - Lee, Tae-Jin
AU  - Lee TJ
AUID- ORCID: 0000-0001-6091-542X
AD  - School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
AD  - Department of Bio-Health Convergence, Kangwon National University, Chuncheon 
      24341, Korea.
FAU - Jeong, Gun-Jae
AU  - Jeong GJ
AD  - School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 
      30332, USA.
FAU - Song, Jihun
AU  - Song J
AD  - School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
FAU - Yu, Taekyung
AU  - Yu T
AUID- ORCID: 0000-0003-4703-3523
AD  - Department of Chemical Engineering, Kyung Hee University, Youngin 17104, Korea.
FAU - Lee, Doo Sung
AU  - Lee DS
AD  - School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
AD  - Theranostic Macromolecules Research Center, Sungkyunkwan University, Suwon 16419, 
      Korea.
FAU - Bhang, Suk Ho
AU  - Bhang SH
AD  - School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
LA  - eng
GR  - NRF-2016M3A9B4919711/National Research Foundation of Korea/
GR  - NRF-2018M3A9E2023255/National Research Foundation of Korea/
GR  - NRF-2019R1C1C1007384/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20210120
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC7864336
OTO - NOTNLM
OT  - angiogenesis
OT  - enzyme free cell detachment
OT  - hindlimb ischemia
OT  - human adipose tissue-derived stem cells
OT  - pH-sensitive polymer
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/28 06:00
MHDA- 2021/01/28 06:01
PMCR- 2021/01/20
CRDT- 2021/01/27 01:06
PHST- 2020/12/28 00:00 [received]
PHST- 2021/01/11 00:00 [revised]
PHST- 2021/01/18 00:00 [accepted]
PHST- 2021/01/27 01:06 [entrez]
PHST- 2021/01/28 06:00 [pubmed]
PHST- 2021/01/28 06:01 [medline]
PHST- 2021/01/20 00:00 [pmc-release]
AID - ma14030491 [pii]
AID - materials-14-00491 [pii]
AID - 10.3390/ma14030491 [doi]
PST - epublish
SO  - Materials (Basel). 2021 Jan 20;14(3):491. doi: 10.3390/ma14030491.