PMID- 33498689
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 3
DP  - 2021 Jan 23
TI  - Tear-Derived Exosome Proteins Are Increased in Patients with Thyroid Eye Disease.
LID - 10.3390/ijms22031115 [doi]
LID - 1115
AB  - Exosomes contain proteins, lipids, RNA, and DNA that mediate intercellular 
      signaling. Exosomes can contribute to the pathological processes of various 
      diseases, although their roles in ocular diseases are unclear. We aimed to 
      isolate exosomes from tear fluids (TF) of patients with Thyroid eye disease (TED) 
      and analyze the exosomal proteins. TFs were collected from eight patients with 
      TED and eight control subjects. The number of TF exosomes were measured using 
      nanoparticle-tracking analysis. The expression of specific proteins in the 
      purified exosome pellets were analyzed using a Proteome Profiler Array Kit. 
      Cultured normal orbital fibroblasts were incubated with TF exosomes from patients 
      with TED and control subjects, and changes in inflammatory cytokine levels were 
      compared. TF exosomes from TED patients showed more exosomes than the control 
      subjects. The expression levels of exosomal proteins vitamin D-binding (VDB) 
      protein, C-reactive protein (CRP), chitinase 3-like 1 (CHI3L1), matrix 
      metalloproteinase-9 (MMP-9), and vascular adhesion molecule-1 (VCAM-1) were 
      significantly increased in patients with TED, compared to those of controls. 
      Orbital fibroblasts exposed to TF exosomes from patients with TED showed 
      significantly higher levels of interleukin (IL)-6, IL-8, and monocyte 
      chemoattractant protein-1 (MCP-1) production than those treated with control TF 
      exosomes. Specific proteins showed higher expression in exosomes from TED 
      patients, implying that they may play keys roles in TED pathogenesis.
FAU - Han, Jeong-Sun
AU  - Han JS
AUID- ORCID: 0000-0002-4491-626X
AD  - Department of Internal Medicine, Division of Endocrinology and Metabolism, Seoul 
      St. Mary Hospital, College of Medicine, The Catholic University of Korea, Seoul 
      06591, Korea.
FAU - Kim, Sung Eun
AU  - Kim SE
AD  - Department of Ophthalmology and Visual Science, Seoul St. Mary Hospital, College 
      of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Jin, Jun-Qing
AU  - Jin JQ
AUID- ORCID: 0000-0002-5018-7008
AD  - Department of Internal Medicine, Division of Endocrinology and Metabolism, Seoul 
      St. Mary Hospital, College of Medicine, The Catholic University of Korea, Seoul 
      06591, Korea.
FAU - Park, Na Ri
AU  - Park NR
AD  - Department of Ophthalmology and Visual Science, Seoul St. Mary Hospital, College 
      of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Lee, Ji-Young
AU  - Lee JY
AD  - Department of Pathology, Institute of Hansen's Disease, Seoul St. Mary Hospital, 
      College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Kim, Hong Lim
AU  - Kim HL
AD  - Integrative Research Support Center, Laboratory of Electron Microscope, College 
      of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Lee, Seong-Beom
AU  - Lee SB
AD  - Department of Pathology, Institute of Hansen's Disease, Seoul St. Mary Hospital, 
      College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Yang, Suk-Woo
AU  - Yang SW
AD  - Department of Ophthalmology and Visual Science, Seoul St. Mary Hospital, College 
      of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
FAU - Lim, Dong-Jun
AU  - Lim DJ
AUID- ORCID: 0000-0003-0995-6482
AD  - Department of Internal Medicine, Division of Endocrinology and Metabolism, Seoul 
      St. Mary Hospital, College of Medicine, The Catholic University of Korea, Seoul 
      06591, Korea.
LA  - eng
GR  - 2019R1I1A1A01059472/the Basic Science Research Program through the National 
      Research Foundation of Korea (NRF) funded by the Ministry of Education/
PT  - Journal Article
DEP - 20210123
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (CHI3L1 protein, human)
RN  - 0 (Chitinase-3-Like Protein 1)
RN  - 0 (Cytokines)
RN  - 0 (Eye Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Vitamin D-Binding Protein)
RN  - 0 (tear proteins)
RN  - 554Z48XN5E (Methimazole)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Chitinase-3-Like Protein 1/analysis/metabolism
MH  - Cytokines/analysis/metabolism
MH  - Exosomes/*chemistry/pathology
MH  - Eye Proteins/analysis/*metabolism
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Graves Ophthalmopathy/drug therapy/*pathology
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 9/analysis/metabolism
MH  - Methimazole/therapeutic use
MH  - Middle Aged
MH  - Tears/*cytology/metabolism
MH  - Vascular Cell Adhesion Molecule-1/analysis/metabolism
MH  - Vitamin D-Binding Protein/analysis/metabolism
PMC - PMC7866068
OTO - NOTNLM
OT  - Graves' ophthalmopathy
OT  - eear fluids
OT  - ehyroid-associated ophthalmopathy
OT  - exosomes
OT  - extracellular vesicle
OT  - eytokines
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/28 06:00
MHDA- 2021/09/04 06:00
PMCR- 2021/01/23
CRDT- 2021/01/27 01:07
PHST- 2020/12/23 00:00 [received]
PHST- 2021/01/14 00:00 [revised]
PHST- 2021/01/20 00:00 [accepted]
PHST- 2021/01/27 01:07 [entrez]
PHST- 2021/01/28 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2021/01/23 00:00 [pmc-release]
AID - ijms22031115 [pii]
AID - ijms-22-01115 [pii]
AID - 10.3390/ijms22031115 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Jan 23;22(3):1115. doi: 10.3390/ijms22031115.