PMID- 33498972
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210316
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 10
IP  - 2
DP  - 2021 Jan 22
TI  - Evidence for Establishing the Clinical Breakpoint of Cefquinome against 
      Haemophilus Parasuis in China.
LID - 10.3390/pathogens10020105 [doi]
LID - 105
AB  - Haemophilus parasuis can cause high morbidity and mortality in swine. Cefquinome 
      possesses excellent antibacterial activity against pathogens causing diseases of 
      the respiratory tract. This study aimed to establish the clinical breakpoint 
      (CBP) of cefquinome against H. parasuis and to monitor the resistance change. 
      Referring to the minimum inhibitory concentration (MIC) distribution of 
      cefquinome against 131 H. parasuis isolates, the MIC(50) and MIC(90) were 
      determined to be 0.125 and 1 mug/mL, respectively. And the epidemiological cutoff 
      (ECOFF) value was 1 mug/mL. HPS42 was selected as a representative strain for the 
      pharmacodynamic (PD) experiment, pharmacokinetic (PK) experiment and clinical 
      experiments. The PK/PD index values, area under concentration-time curve 
      (AUC)/MIC, of the bacteriostatic, bactericidal, and bacterial elimination effects 
      were 23, 41, and 51 h, respectively. The PK/PD cutoff was calculated as 0.125 
      mug/mL by Monte Carlo simulation (MCS), and the clinical cutoff was 0.25-4 mug/mL 
      by WindoW. Combing these three values, the CBP of cefquinome against H. parasuis 
      was found to be 1 mug/mL. In conclusion, this was the first study to integrate 
      various cutoffs to establish the CBP in the laboratory. It is helpful to 
      distinguish wild type H. parasuis and reduce the probability of treatment 
      failure.
FAU - Mi, Kun
AU  - Mi K
AUID- ORCID: 0000-0001-7058-1849
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
FAU - Sun, Da
AU  - Sun D
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
FAU - Li, Mei
AU  - Li M
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
FAU - Hao, Haihong
AU  - Hao H
AUID- ORCID: 0000-0003-0199-5700
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
FAU - Zhou, Kaixiang
AU  - Zhou K
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
FAU - Liu, Zhenli
AU  - Liu Z
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
FAU - Yuan, Zonghui
AU  - Yuan Z
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
AD  - MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry 
      Products, Huazhong Agricultural University, Wuhan 430000, China.
FAU - Huang, Lingli
AU  - Huang L
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU), Wuhan 430000, 
      China.
AD  - MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong 
      Agricultural University, Wuhan 430000, China.
AD  - MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry 
      Products, Huazhong Agricultural University, Wuhan 430000, China.
LA  - eng
GR  - 2016YFD0501310/National key research and development program/
GR  - 2662017PY081/Fundamental research funds for the Central Universities/
PT  - Journal Article
DEP - 20210122
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC7912692
OTO - NOTNLM
OT  - Haemophilus parasuis
OT  - PK/PD cutoff
OT  - cefquinome
OT  - clinical breakpoint
OT  - clinical cutoff
OT  - epidemiological cutoff
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/01/28 06:00
MHDA- 2021/01/28 06:01
PMCR- 2021/01/22
CRDT- 2021/01/27 01:07
PHST- 2020/11/30 00:00 [received]
PHST- 2021/01/05 00:00 [revised]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2021/01/27 01:07 [entrez]
PHST- 2021/01/28 06:00 [pubmed]
PHST- 2021/01/28 06:01 [medline]
PHST- 2021/01/22 00:00 [pmc-release]
AID - pathogens10020105 [pii]
AID - pathogens-10-00105 [pii]
AID - 10.3390/pathogens10020105 [doi]
PST - epublish
SO  - Pathogens. 2021 Jan 22;10(2):105. doi: 10.3390/pathogens10020105.