PMID- 33499235
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210210
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 10
IP  - 3
DP  - 2021 Jan 22
TI  - Endothelin A Receptors Expressed in Glomeruli of Renal Transplant Patients May Be 
      Associated with Antibody-Mediated Rejection.
LID - 10.3390/jcm10030422 [doi]
LID - 422
AB  - BACKGROUND: Non-human leukocyte antigen (HLA) anti-endothelin A receptor 
      antibodies are presented as being potentially important, but the expression of 
      the endothelin A receptor in glomeruli (ETA receptor (g+)) has not yet been 
      described. We decided to evaluate the presence and relevance of the ETA receptor 
      in for-cause renal transplant biopsies. The aim of our study was to evaluate the 
      immunoreactivity of the ETA receptor and its significance in patients who 
      underwent a renal transplant biopsy due to the deterioration of transplant 
      function, with detailed characterization of staining in glomeruli. METHODS: The 
      immunohistochemical expression of ETA receptor (ETAR) was analyzed in renal 
      transplant biopsies. Microscopic evaluation was performed on paraffin sections in 
      glomeruli. The analysis was performed using a two-step scale (0: lack of ETAR 
      expression; 1: the presence of ETAR expression-mild to moderate 
      immunoreactivity). RESULTS: We analyzed 149 patients who underwent renal 
      allograft biopsy after renal transplantation. Positive staining of ETA receptors 
      in glomeruli (ETA receptor (g+)) was noticed in 13/149 (8.7%) patients. Five of 
      these 13 (38.5%) patients with ETA receptor (g+) developed antibody-mediated 
      rejection (AMR), while 13 of the remaining 136 (9.5%) ETA receptor (g-) patients 
      developed AMR (p = 0.0022). Graft loss was noticed in all but one ETA receptor 
      (g+) patient with AMR (4/5; 80%), but only in 2/13 (15%) ETA receptor (g-) 
      patients with AMR (p = 0.009) during the first year after biopsy. CONCLUSIONS: 
      The expression of endothelin A receptors in glomeruli seems to be a potentially 
      important feature in the diagnosis of damage during antibody-mediated rejection. 
      It may help to identify patients at a higher risk of allograft rejection and 
      injury.
FAU - Nowanska, Katarzyna
AU  - Nowanska K
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Banasik, Miroslaw
AU  - Banasik M
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Donizy, Piotr
AU  - Donizy P
AD  - Department of Pathomorphology and Oncological Cytology, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Koscielska-Kasprzak, Katarzyna
AU  - Koscielska-Kasprzak K
AD  - Research laboratory, Wroclaw Medical University, 50-367 Wroclaw, Poland.
FAU - Zmonarski, Slawomir
AU  - Zmonarski S
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Letachowicz, Krzysztof
AU  - Letachowicz K
AUID- ORCID: 0000-0002-2747-8851
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Kaminska, Dorota
AU  - Kaminska D
AUID- ORCID: 0000-0003-2400-9328
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Mazanowska, Oktawia
AU  - Mazanowska O
AUID- ORCID: 0000-0001-7669-7543
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Augustyniak-Bartosik, Hanna
AU  - Augustyniak-Bartosik H
AUID- ORCID: 0000-0003-3969-396X
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Tukiendorf, Andrzej
AU  - Tukiendorf A
AD  - Department of Public Health, Wroclaw Medical University, 50-367 Wroclaw, Poland.
FAU - Chudiak, Anna
AU  - Chudiak A
AD  - Division of Nursing in Internal Medicine Procedures, Department of Clinical 
      Nursing, Faculty of Health Sciences, Wroclaw Medical University, 50-367 Wroclaw, 
      Poland.
FAU - Dawiskiba, Tomasz
AU  - Dawiskiba T
AUID- ORCID: 0000-0003-2329-2918
AD  - Department of General, Vascular and Transplant Surgery, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Halon, Agnieszka
AU  - Halon A
AD  - Department of Pathomorphology and Oncological Cytology, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
FAU - Krajewska, Magdalena
AU  - Krajewska M
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, 50-367 Wroclaw, Poland.
LA  - eng
GR  - STM.C160.20.155/The research was financially supported by the Ministry of Health 
      subvention according to number from the IT Simple system of the Wroclaw Medical 
      University./
PT  - Journal Article
DEP - 20210122
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7865600
OTO - NOTNLM
OT  - allograft injury
OT  - antibody-mediated rejection
OT  - endothelin A receptors
OT  - non-HLA antibodies
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/28 06:00
MHDA- 2021/01/28 06:01
PMCR- 2021/01/22
CRDT- 2021/01/27 01:08
PHST- 2020/11/24 00:00 [received]
PHST- 2021/01/02 00:00 [revised]
PHST- 2021/01/18 00:00 [accepted]
PHST- 2021/01/27 01:08 [entrez]
PHST- 2021/01/28 06:00 [pubmed]
PHST- 2021/01/28 06:01 [medline]
PHST- 2021/01/22 00:00 [pmc-release]
AID - jcm10030422 [pii]
AID - jcm-10-00422 [pii]
AID - 10.3390/jcm10030422 [doi]
PST - epublish
SO  - J Clin Med. 2021 Jan 22;10(3):422. doi: 10.3390/jcm10030422.