PMID- 33500150 OWN - NLM STAT- MEDLINE DCOM- 20210423 LR - 20210423 IS - 1879-0097 (Electronic) IS - 0109-5641 (Linking) VI - 37 IP - 3 DP - 2021 Mar TI - Resin-based dental materials containing 3-aminopropyltriethoxysilane modified halloysite-clay nanotubes for extended drug delivery. PG - 508-515 LID - S0109-5641(21)00006-3 [pii] LID - 10.1016/j.dental.2020.12.011 [doi] AB - OBJECTIVE: To synthesize and characterize a novel resin-based dental material containing 3-aminopropyltriethoxysilane (APTES) surface-modified halloysite-clay nanotubes (HNTs) for long-term delivery of guest molecules. METHODS: The optimal concentrations of HNT (10, 15, 20 wt.%) and silane (0, 2, 4 vol.%sil) to be incorporated into the resin-based materials were determined (15 wt.%HNT, 4 vol.%sil) after assessment of the mechanical properties (DC%, degree of conversion; FS, flexural strength; FM, flexural modulus; and UTS, ultimate tensile strength). The HNTsil-powder was loaded with chlorhexidine (CHX) to evaluate the effect of the silanization on drug release. Resin-discs were prepared for the following groups: RES (resin), HNT (resin+15 wt.%HNT), HNTsil (resin+15 wt.%HNT silanized), HNT-CHX (resin+15 wt.%HNT loaded with chlorhexidine), HNTsil-CHX (resin+15 wt.%HNTsil-CHX), and 0.2 vol.%CHX (resin+0.2 vol.%CHX solution). Specimens were stored in water for 1, 3, 5, 10, and 15 days at 37 degrees C. Aliquots from each time point and the final 15-day specimens were evaluated for the zone of inhibition (ZOI) against Streptococcus mutans. CHX release was analyzed using spectrophotometry at absorbance of 300 nm. Data were statistically analyzed (alpha = 0.05). RESULTS: All materials presented similar DC%. Reduced FS but increased FM was detected for 20 wt.%HNT-4%APTES. Groups with 15 wt.% and 20 wt.%HNT with/without APTES presented higher values of UTS. Agar diffusion data indicates that the HNTsil-CHX had a greater ZOI than all other groups over 15 days. HNTsil-CHX had the highest absorbance for day 1 but presented similar values to other groups every time point after. SIGNIFICANCE: Silanization of nanotubes followed by encapsulation of chlorhexidine is a promising technique for long-term delivery of guest molecules. CI - Copyright (c) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. FAU - Karczewski, Ashley AU - Karczewski A AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA. Electronic address: aekarcze@iu.edu. FAU - Kalagi, Sara AU - Kalagi S AD - Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA; Department of Restorative and Prosthetic Dental Sciences, College of Dentistry, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. Electronic address: Kalajis@ksau-hs.edu.sa. FAU - Viana, Itallo Emidio Lira AU - Viana IEL AD - Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, 05508-000 SP, Brazil. Electronic address: italloviana@usp.br. FAU - Martins, Victor Mota AU - Martins VM AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA; Department of Operative Dentistry and Dental Materials, Federal University of Uberlandia, UFU, Minas Gerais, Brazil; Department of Operative Dentistry, School of Dentistry, Faculdade Patos de Minas (FPM), Patos de Minas, Minas Gerais, 38700-001, Brazil. Electronic address: victor.martins@faculdadepatosdeminas.edu.br. FAU - Duarte, Simone AU - Duarte S AD - Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA. Electronic address: siduarte@iu.edu. FAU - Gregory, Richard L AU - Gregory RL AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA. Electronic address: rgregory@iu.edu. FAU - P Youngblood, Jeffrey AU - P Youngblood J AD - School of Materials Engineering, Purdue University, 701 West Stadium Ave., West Lafayette, IN 47907-2045, USA. Electronic address: jpyoungb@purdue.edu. FAU - Platt, Jeffrey A AU - Platt JA AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA. Electronic address: jplatt2@iu.edu. FAU - Feitosa, Sabrina AU - Feitosa S AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry (IUSD), Indianapolis, IN 46202, USA. Electronic address: sfeitosa@iu.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210123 PL - England TA - Dent Mater JT - Dental materials : official publication of the Academy of Dental Materials JID - 8508040 RN - 0 (Dental Materials) RN - 0 (Pharmaceutical Preparations) RN - 0 (Propylamines) RN - 0 (Silanes) RN - L8S6UBW552 (amino-propyl-triethoxysilane) RN - R4KO0DY52L (Chlorhexidine) RN - T1FAD4SS2M (Clay) MH - Chlorhexidine MH - Clay MH - Dental Materials MH - Materials Testing MH - *Nanotubes MH - *Pharmaceutical Preparations MH - Propylamines MH - Silanes OTO - NOTNLM OT - Chlorhexidine OT - Inhibition zone OT - Nanotubes OT - Resin-based materials OT - Silanization EDAT- 2021/01/28 06:00 MHDA- 2021/04/24 06:00 CRDT- 2021/01/27 05:38 PHST- 2020/08/17 00:00 [received] PHST- 2020/12/13 00:00 [revised] PHST- 2020/12/30 00:00 [accepted] PHST- 2021/01/28 06:00 [pubmed] PHST- 2021/04/24 06:00 [medline] PHST- 2021/01/27 05:38 [entrez] AID - S0109-5641(21)00006-3 [pii] AID - 10.1016/j.dental.2020.12.011 [doi] PST - ppublish SO - Dent Mater. 2021 Mar;37(3):508-515. doi: 10.1016/j.dental.2020.12.011. Epub 2021 Jan 23.