PMID- 33504684
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210409
IS  - 2093-3665 (Print)
IS  - 2093-3673 (Electronic)
IS  - 2093-3665 (Linking)
VI  - 54
IP  - 1
DP  - 2021 Mar 31
TI  - Anti-proliferative and apoptotic effects of hull-less pumpkin extract on human 
      papillary thyroid carcinoma cell line.
PG  - 104-111
LID - 10.5115/acb.20.228 [doi]
AB  - Papillary thyroid carcinoma (PTC) is one of the most common cancers of the 
      endocrine system. Previous studies have shown that the extract of hull-less 
      pumpkin seed (HLPS) has a significant anti-cancer effect. The aim of this study 
      was to evaluate the effect of this plant extract on the proliferation of PTC 
      cells. In this study, an extract of this plant was prepared by soxhlet extraction 
      method and analyzed by Gas Chromatography-Mass Spectrometry. The cytotoxicity of 
      PTX and plant extract was investigated using the methylthiazol tetrazolium (MTT) 
      method. For careful investigation of morphological alteration, we used 
      hematoxylin and eosin and Giemsa stinging. Based on MTT assay test, the IC(50) 
      value of paclitaxel (PTX) was significantly less than the hydro-alcoholic extract 
      of HLPS at all of the incubation time. Our results of histological staining 
      showed that HLPS and PTX induced significant morphological alteration in the PTC 
      cultured cell that consistent with cell death. Comparing the groups treated by 
      PTX or HLPS with control group showed significant differences. It seems that HLPS 
      extract has an apparent effect on treatment of PTC, at least in laboratory 
      condition, albeit for realistic decision about the effect of HLPS on PTC, more 
      molecular investigations are necessary.
FAU - Bahadori, Mohammad Hadi
AU  - Bahadori MH
AUID- ORCID: 0000-0001-9840-0229
AD  - Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of 
      Medical Sciences, Rasht, Iran.
FAU - Azari, Zoleikha
AU  - Azari Z
AUID- ORCID: 0000-0001-5923-0482
AD  - Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of 
      Medical Sciences, Rasht, Iran.
FAU - Zaminy, Arash
AU  - Zaminy A
AUID- ORCID: 0000-0002-2388-481X
AD  - Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of 
      Medical Sciences, Rasht, Iran.
FAU - Dabirian, Sara
AU  - Dabirian S
AUID- ORCID: 0000-0002-7945-6558
AD  - Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University 
      of Medical Sciences, Rasht, Iran.
FAU - Mehrdad, Seyed Mojtaba
AU  - Mehrdad SM
AUID- ORCID: 0000-0003-2641-452X
AD  - Department of Endocrinology, Guilan University of Medical Sciences, Rasht, Iran.
FAU - Kondori, Bahman Jalali
AU  - Kondori BJ
AUID- ORCID: 0000-0002-6134-9965
AD  - Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University 
      of Medical Sciences, Tehran, Iran.
AD  - Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), 
      Baqiyatallah University of Medical Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Anat Cell Biol
JT  - Anatomy & cell biology
JID - 101531987
PMC - PMC8017459
OTO - NOTNLM
OT  - Cell proliferation
OT  - Herbal medicine
OT  - Paclitaxel
OT  - Thyroid neoplasms
COIS- Conflicts of Interest No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2021/01/29 06:00
MHDA- 2021/01/29 06:01
PMCR- 2021/03/31
CRDT- 2021/01/28 05:34
PHST- 2020/08/26 00:00 [received]
PHST- 2020/11/30 00:00 [revised]
PHST- 2020/12/14 00:00 [accepted]
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/01/29 06:01 [medline]
PHST- 2021/01/28 05:34 [entrez]
PHST- 2021/03/31 00:00 [pmc-release]
AID - acb.20.228 [pii]
AID - acb-54-1-104 [pii]
AID - 10.5115/acb.20.228 [doi]
PST - ppublish
SO  - Anat Cell Biol. 2021 Mar 31;54(1):104-111. doi: 10.5115/acb.20.228.