PMID- 33505602
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231110
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 12
IP  - 12
DP  - 2020 Dec 26
TI  - Spinal cord injury regeneration using autologous bone marrow-derived neurocytes 
      and rat embryonic stem cells: A comparative study in rats.
PG  - 1591-1602
LID - 10.4252/wjsc.v12.i12.1591 [doi]
AB  - BACKGROUND: Spinal cord injury (SCI) is an important cause of traumatic paralysis 
      and is mainly due to motor vehicle accidents. However, there is no definite 
      treatment for spinal cord damage. AIM: To assess the outcome of rat embryonic 
      stem cells (rESC) and autologous bone marrow-derived neurocytes (ABMDN) treatment 
      in iatrogenic SCI created in rats, and to compare the efficacy of the two 
      different cell types. METHODS: The study comprised 45 male Wistar rats weighing 
      between 250 and 300 g, which were divided into three groups, the control, rESC 
      and ABMDN groups. The anesthetized animals underwent exposure of the thoracic 
      8(th) to lumbar 1(st) vertebrae. A T10-thoracic 12(th) vertebrae laminectomy was 
      performed to expose the spinal cord. A drop-weight injury using a 10 g weight 
      from a height of 25 cm onto the exposed spinal cord was conducted. The wound was 
      closed in layers. The urinary bladder was manually evacuated twice daily and 
      after each evacuation Ringer lactate 5 mL/100 g was administered, twice daily 
      after each bladder evacuation for the first 7 postoperative days. On the 10(th) 
      day, the rats underwent nerve conduction studies and behavioral assessment 
      [Basso, Beattie, Brenham (BBB)] to confirm paraplegia. Rat embryonic stem cells, 
      ABMDN and saline were injected on the 10(th) day. The animals were euthanized 
      after 8 wk and the spinal cord was isolated, removed and placed in 2% formalin 
      for histopathological analysis to assess the healing of neural tissues at the 
      axonal level. RESULTS: All the animals tolerated the procedure well. The BBB 
      scale scoring showed that at the end of the first week no recovery was observed 
      in the groups. Post-injection, there was a strong and significant improvement in 
      rats receiving rESC and ABMDN as compared to the control group based on the BBB 
      scale, and the Train-of-four-Watch SX acceleromyography device exhibited 
      statistically significant (P < 0.0001) regeneration of neural tissue after SCI. 
      Histological evaluation of the spinal cord showed maximum vacuolization and least 
      gliosis in the control group compared to the rESC and ABMDN treated animals. In 
      the ABMDN group, limited vacuolization and more prominent gliosis were observed 
      in all specimens as compared to the control and rESC groups. CONCLUSION: This 
      study provided strong evidence to support that transplantation of rESC and ABMDN 
      can improve functional recovery after iatrogenic SCI. The transplanted cells 
      showed a beneficial therapeutic effect when compared to the control group.
CI  - (c)The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights 
      reserved.
FAU - Sadat-Ali, Mir
AU  - Sadat-Ali M
AD  - Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University and King 
      Fahd Hospital of the University, Dammam 31952, Saudi Arabia. drsadat@hotmail.com.
FAU - Al-Dakheel, Dakheel A
AU  - Al-Dakheel DA
AD  - Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University, 
      AlKhobar 31952, Saudi Arabia.
FAU - Ahmed, Ayesha
AU  - Ahmed A
AD  - Department of Pathology, Imam Abdulrahman Bin Faisal University, Dammam 31952, 
      Saudi Arabia.
FAU - Al-Turki, Haifa A
AU  - Al-Turki HA
AD  - Department of Obstetrics and Gynecology, Imam Abdulrahman Bin Faisal University, 
      Dammam 31142, Saudi Arabia.
FAU - Al-Omran, Abdallah S
AU  - Al-Omran AS
AD  - Department of Orthopedic Surgery, Imam Abdulrahman Bin Faisal University and King 
      Fahd Hospital of the University, AlKhobar 31952, Saudi Arabia.
FAU - Acharya, Sadananda
AU  - Acharya S
AD  - Department of Public Health, College of Public Health, Imam Abdulrahman Bin 
      Faisal University, Dammam 31441, Saudi Arabia.
FAU - Al-Bayat, Methal I
AU  - Al-Bayat MI
AD  - Department of Pathology, Imam Abdulrahman Bin Faisal University, Dammam 31952, 
      Saudi Arabia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC7789116
OTO - NOTNLM
OT  - Autologous bone marrow-derived neurocytes
OT  - Embryonic stem cells
OT  - Iatrogenic
OT  - Regeneration
OT  - Spinal cord injury
OT  - Stem cells
COIS- Conflict-of-interest statement: All authors have no conflicts of interest related 
      to the manuscript.
EDAT- 2021/01/29 06:00
MHDA- 2021/01/29 06:01
PMCR- 2020/12/26
CRDT- 2021/01/28 05:42
PHST- 2020/03/21 00:00 [received]
PHST- 2020/08/24 00:00 [revised]
PHST- 2020/11/12 00:00 [accepted]
PHST- 2021/01/28 05:42 [entrez]
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/01/29 06:01 [medline]
PHST- 2020/12/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v12.i12.1591 [doi]
PST - ppublish
SO  - World J Stem Cells. 2020 Dec 26;12(12):1591-1602. doi: 10.4252/wjsc.v12.i12.1591.