PMID- 33505935
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210130
IS  - 2287-1098 (Print)
IS  - 2287-8602 (Electronic)
IS  - 2287-1098 (Linking)
VI  - 25
IP  - 4
DP  - 2020 Dec 31
TI  - Inhibitory Effect of Black Radish (Raphanus sativus L. var. niger) Extracts on 
      Lipopolysaccharide-Induced Inflammatory Response in the Mouse 
      Monocyte/Macrophage-Like Cell Line RAW 264.7.
PG  - 408-421
LID - 10.3746/pnf.2020.25.4.408 [doi]
AB  - Black radish (Raphanus sativus L. var. niger), which is cultivated worldwide, is 
      used in traditional medicine as it aids liver function, gastric secretion, 
      gallbladder function, and gallstone mitigation. In this study, we examined the 
      anti-inflammatory effects of black radish extract (BRE) on the lipopolysaccharide 
      (LPS)- and interleukin (IL)-6-mediated inflammatory responses in the RAW 264.7 
      cell lines. Our findings show that BRE significantly ameliorated LPS-induced 
      nitric oxide (NO) release and production of pro-inflammatory cytokines, such as 
      IL-1beta, IL-6, tumor necrosis factor (TNF)-alpha, and prostaglandin E2. The levels of 
      cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) in LPS-stimulated RAW 
      264.7 cells were found to be suppressed by BRE. Further, BRE significantly 
      suppressed the LPS-induced expression of mRNAs encoding COX-2, iNOS, IL-1beta, IL-6, 
      and TNF-alpha in a concentration-dependent manner. BRE treatment significantly 
      inhibited Janus kinase 2 (JAK2) and signal transducer and activator of 
      transcription 3 (STAT3) phosphorylation in IL-6- and LPS-treated RAW 264.7 cells. 
      In addition, BRE decreased the levels of phosphorylated extracellular 
      signal-regulated protein kinases and c-Jun N-terminal kinase under the same 
      conditions. Moreover, BRE induced high nuclear factor erythroid 2-related factor 
      2 (NRF2) levels and its target gene heme oxygenase 1 (HO-1) in the absence of 
      LPS. These data demonstrate that BRE may be beneficial for treating inflammation 
      through selective immunomodulatory effects, which may be mediated by inhibition 
      of the STAT3/JAK2 and activation of the NRF2/HO-1 signal transduction pathways.
CI  - Copyright (c) 2020 by The Korean Society of Food Science and Nutrition. All rights 
      Reserved.
FAU - Jeon, Hyungsik
AU  - Jeon H
AD  - Biodiversity Research Institute, Jeju Technopark, Jeju 63608, Korea.
FAU - Yang, Dawun
AU  - Yang D
AD  - Research Team, Creation & Innovation Research Institute, IT'S HANBUL Co., Ltd., 
      Seoul 06101, Korea.
FAU - Lee, Nam Ho
AU  - Lee NH
AD  - Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, 
      Korea.
FAU - Ahn, Meejung
AU  - Ahn M
AD  - Department of Animal Science, College of Life Science, Sangji University, Gangwon 
      26339, Korea.
FAU - Kim, Giok
AU  - Kim G
AD  - Biodiversity Research Institute, Jeju Technopark, Jeju 63608, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Prev Nutr Food Sci
JT  - Preventive nutrition and food science
JID - 101586663
PMC - PMC7813598
OTO - NOTNLM
OT  - JAK2/STAT3
OT  - LPS/IL-6
OT  - NRF2
OT  - anti-inflammatory effects
OT  - black radish
COIS- AUTHOR DISCLOSURE STATEMENT The authors declare no conflict of interest.
EDAT- 2021/01/29 06:00
MHDA- 2021/01/29 06:01
PMCR- 2020/12/31
CRDT- 2021/01/28 05:44
PHST- 2020/09/18 00:00 [received]
PHST- 2020/10/26 00:00 [accepted]
PHST- 2021/01/28 05:44 [entrez]
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/01/29 06:01 [medline]
PHST- 2020/12/31 00:00 [pmc-release]
AID - PNFS-25-408 [pii]
AID - 10.3746/pnf.2020.25.4.408 [doi]
PST - ppublish
SO  - Prev Nutr Food Sci. 2020 Dec 31;25(4):408-421. doi: 10.3746/pnf.2020.25.4.408.