PMID- 33506649
OWN - NLM
STAT- MEDLINE
DCOM- 20211115
LR  - 20211115
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 24
IP  - 3
DP  - 2021 Mar
TI  - MicroRNA-320c inhibits articular chondrocytes proliferation and induces apoptosis 
      by targeting mitogen-activated protein kinase 1 (MAPK1).
PG  - 402-410
LID - 10.1111/1756-185X.14053 [doi]
AB  - AIM: To clarify the interaction of microRNA-320c (miR-320c) and mitogen-activated 
      protein kinase 1 (MAPK1), and to investigate the effects of miR-320c on articular 
      chondroctye proliferation and apoptosis. METHODS: Lentiviral expression vectors 
      were constructed and dual luciferase assays containing MAPK1 3'-untranslated 
      regions (3'-UTRs) were performed. Small hairpin RNA (shRNA) was utilized to 
      modulate MAPK1 expression. The messenger RNA and protein expression levels were 
      determined by quantitative real-time polymerase chain reaction (qRT-PCR) and 
      western blotting respectively. Cell Counting Kit-8 and flow cytometry were 
      conducted to detect the proliferation and apoptosis of Human 
      Chondrocyte-articular (HC-a) cells. Besides that, the influences of miR-320c and 
      MAPK1 on MAPK pathway activation were also evaluated. RESULTS: Our data 
      identified MAPK1 as a direct target gene of miR-320c, and miR-320c can negatively 
      regulate MAPK1 expression by directly binding to MAPK1 3'-UTR in HC-a cells. 
      Further functional study displayed that miR-320c overexpression and MAPK1 shRNA 
      significantly suppressed the proliferation of HC-a cells and promoted cell 
      apoptosis. Meanwhile, MAPK1 shRNA could attenuate miR-320c inhibitor promotive 
      effects on HC-a cell proliferation and reverse its inhibitory effect on cell 
      apoptosis. MAPK1 overexpression could rescue the inhibitory effect of miR-320c on 
      HC-a cell proliferation, and weaken the accelerating effect of miR-320c on cell 
      apoptosis. However, neither miR-320c or MAPK1 shRNA regulate the expression of 
      c-JUN, JNK and c-Fos. CONCLUSION: miR-320c inhibits articular chondrocyte 
      proliferation and induces apoptosis by targeting MAPK1, suggesting that miR-320c 
      perhaps participates in the pathogenesis of osteoarthritis and acts as a 
      potential target for the therapeutic treatment of osteoarthritis.
CI  - (c) 2021 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons 
      Australia, Ltd.
FAU - Zhao, Like
AU  - Zhao L
AUID- ORCID: 0000-0001-9408-6623
AD  - Department of Rheumatology, Beijing Hospital, National Center of Gerontology, 
      Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Zhou, Rongwei
AU  - Zhou R
AD  - Department of Respiratory and Critical Care Medicine, Shanghai Sixth People's 
      Hospital, Shanghai Jiaotong University, Shanghai, China.
FAU - Wang, Qian
AU  - Wang Q
AD  - Department of Rheumatology, Beijing Hospital, National Center of Gerontology, 
      Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Cheng, Yongjing
AU  - Cheng Y
AD  - Department of Rheumatology, Beijing Hospital, National Center of Gerontology, 
      Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Gao, Ming
AU  - Gao M
AD  - Department of Rheumatology, Beijing Hospital, National Center of Gerontology, 
      Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Huang, Cibo
AU  - Huang C
AD  - Department of Rheumatology, Beijing Hospital, National Center of Gerontology, 
      Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 
      China.
LA  - eng
GR  - BJ-2018-003/Beijing Hospital Research Foundation/
PT  - Journal Article
DEP - 20210127
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (MIRN320 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Small Interfering)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.24 (MAPK1 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Cell Count
MH  - Cell Line
MH  - Cell Proliferation
MH  - Chondrocytes/metabolism/*pathology
MH  - DNA/genetics
MH  - *Gene Expression Regulation
MH  - Humans
MH  - MicroRNAs/biosynthesis/*genetics
MH  - Mitogen-Activated Protein Kinase 1/biosynthesis/*genetics
MH  - Osteoarthritis/*genetics/metabolism/pathology
MH  - RNA, Small Interfering/genetics
OTO - NOTNLM
OT  - MAPK1
OT  - MiR-320c
OT  - apoptosis
OT  - cell proliferation
OT  - chondrocytes
EDAT- 2021/01/29 06:00
MHDA- 2021/11/16 06:00
CRDT- 2021/01/28 05:50
PHST- 2020/09/18 00:00 [received]
PHST- 2020/12/09 00:00 [revised]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/01/28 05:50 [entrez]
AID - 10.1111/1756-185X.14053 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2021 Mar;24(3):402-410. doi: 10.1111/1756-185X.14053. Epub 2021 
      Jan 27.