PMID- 33507122
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20220129
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 20
IP  - 4
DP  - 2021 Feb
TI  - Upregulated circRNA hsa_circ_0071036 promotes tumourigenesis of pancreatic cancer 
      by sponging miR-489 and predicts unfavorable characteristics and prognosis.
PG  - 369-382
LID - 10.1080/15384101.2021.1874684 [doi]
AB  - Circular RNAs (circRNAs), the new stars of endogenous non-coding RNAs, are 
      dysregulated in various tumors including pancreatic cancer. Here, we aimed to 
      investigate the biological functions of hsa_circ_0071036 in the tumourigenesis 
      and progression of pancreatic ductal adenocarcinoma (PDAC) and its clinical 
      implications. The differential expression profile of circRNAs in 4 pairs of PDAC 
      tissues was analyzed by microarray assay. Quantitative real-time PCR and 
      fluorescence in situ hybridization (FISH) were utilized to determine the 
      expression patterns and their clinical significance. Functional experiments in 
      vitro and in vivo were performed to explore whether hsa_circ_0071036 functions as 
      an oncogenic circRNA in PDAC. Mechanistically, RT-qPCR, dual luciferase reporter 
      and RNA pull-down assays were conducted to identify the interaction between 
      hsa_circ_0071036 and miR-489 in PDAC. Hsa_circ_0071036 was remarkably 
      overexpressed in PDAC cell lines and tissue samples, which negatively correlated 
      with miR-489 expression. Aberrant expression of hsa_circ_0071036 correlated with 
      poor clinicopathological characteristics and prognoses of PDAC patients. 
      Knockdown of hsa_circ_0071036 suppressed proliferation and invasion and induced 
      apoptosis in vitro. Moreover, the in vivo xenograft model confirmed that 
      silencing of hsa_circ_0071036 attenuated tumor growth. Mechanistic analyses 
      indicated that hsa_circ_0071036 acted as an efficient miRNA sponge for miR-489 in 
      PDAC. In summary, our study revealed that upregulated hsa_circ_0071036 promotes 
      PDAC pathogenesis and progression by directly sponging miR-489, which implies an 
      important role for this circRNA-miRNA functional network.
FAU - Han, Xu
AU  - Han X
AUID- ORCID: 0000-0002-0238-543X
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Fang, Yuan
AU  - Fang Y
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Chen, Pingping
AU  - Chen P
AD  - Department of Assisted Reproduction, Xinhua Hospital, Shanghai Jiaotong 
      University School of Medicine & Molecular Genetics Group, Shanghai Institute for 
      Pediatric Research , Shanghai, China.
FAU - Xu, Yaolin
AU  - Xu Y
AUID- ORCID: 0000-0002-0971-518X
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Zhou, Wentao
AU  - Zhou W
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Rong, Yefei
AU  - Rong Y
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Li, Jian-Ang
AU  - Li JA
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Anesthesia, Zhongshan Hospital, Fudan University , Shanghai, China.
FAU - Lou, Wenhui
AU  - Lou W
AD  - Department of General Surgery, Zhongshan Hospital, Fudan University , Shanghai, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210128
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (MIRN489 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Carcinogenesis/genetics/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*biosynthesis/genetics
MH  - Middle Aged
MH  - Pancreatic Neoplasms/*diagnosis/genetics/*metabolism
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - RNA, Circular/*biosynthesis/genetics
MH  - Survival Rate/trends
MH  - Up-Regulation/*physiology
MH  - Xenograft Model Antitumor Assays/methods
PMC - PMC7894420
OTO - NOTNLM
OT  - Circrnas
OT  - hsa_circ_0071036
OT  - miR-489
OT  - pancreatic cancer
OT  - tumourigenesis
COIS- All authors declare that they have no conflict of interest.
EDAT- 2021/01/29 06:00
MHDA- 2021/12/15 06:00
PMCR- 2022/01/28
CRDT- 2021/01/28 12:12
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/01/28 12:12 [entrez]
PHST- 2022/01/28 00:00 [pmc-release]
AID - 1874684 [pii]
AID - 10.1080/15384101.2021.1874684 [doi]
PST - ppublish
SO  - Cell Cycle. 2021 Feb;20(4):369-382. doi: 10.1080/15384101.2021.1874684. Epub 2021 
      Jan 28.