PMID- 33507382
OWN - NLM
STAT- MEDLINE
DCOM- 20210225
LR  - 20210225
IS  - 1617-4623 (Electronic)
IS  - 1617-4623 (Linking)
VI  - 296
IP  - 2
DP  - 2021 Mar
TI  - Identification of hub lncRNA ceRNAs in multiple sclerosis based on ceRNA 
      mechanisms.
PG  - 423-435
LID - 10.1007/s00438-020-01750-1 [doi]
AB  - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous 
      system, and the pathogenesis is influenced by genetic susceptibility. 
      Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play 
      essential roles in complex diseases, including acting as competing endogenous 
      RNAs (ceRNAs). However, the functional roles and regulatory mechanisms of lncRNAs 
      acting as ceRNAs in MS are still unclear. In this study, we identified hub lncRNA 
      ceRNAs in MS based on ceRNA mechanisms and annotated their functions. The 
      lncRNA-associated ceRNA network (LACN) was constructed by integrating the 
      expression profiles of lncRNA/mRNA and miRNA in MS and normal samples, and the 
      experimentally validated interactions of lncRNA-miRNA and mRNA-miRNA. We found 
      three hub lncRNA ceRNAs (XIST, OIP5-AS1, and CTB-89H12.4) using the network 
      analysis and obtained 96 lncRNA-mediated competing triplets (LCTs, 
      lncRNA-miRNA-mRNA) with the hub lncRNA ceRNAs, which constituted 3 hub ceRNA 
      modules. The functional analysis identified 12 pathways enriched by the 3 hub 
      lncRNA ceRNAs, of which 6 were confirmed to be related to MS. For example, XIST 
      was enriched in the 'spliceosome' and 'RNA transport' related to the typing of 
      MS, and CTB-89H12.4 was enriched in the 'mTOR signaling pathway,' a potential 
      therapeutic target for MS. We dissected the expression patterns of the 96 LCTs in 
      MS individually. LCT XIST-miR-326-HNRNPA1, for which the expression pattern in MS 
      revealed that XIST and HNRNPA1 were up-regulated and miR-326 was down-regulated, 
      consisted of risk RNAs for MS that were validated by other research. Therefore, 
      XIST-miR-326-HNRNPA1 might play a central role in the pathogenesis of MS. These 
      results will contribute to the discovery of novel biomarkers and the development 
      of new therapeutic methods for MS.
FAU - Ding, Yanjun
AU  - Ding Y
AUID- ORCID: 0000-0003-3980-7701
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
AD  - Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Li, Taotao
AU  - Li T
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Yan, Xinwei
AU  - Yan X
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Cui, Mintian
AU  - Cui M
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Wang, Chao
AU  - Wang C
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Wang, Situo
AU  - Wang S
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China.
FAU - Zhang, Fengmin
AU  - Zhang F
AD  - Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, 
      Harbin, 150086, China. fengminzhang@ems.hrbmu.edu.cn.
FAU - Zhang, Ruijie
AU  - Zhang R
AD  - College of Bioinformatics Science and Technology, Harbin Medical University, 
      Harbin, 150086, China. zhangrj@ems.hrbmu.edu.cn.
LA  - eng
GR  - LH2019C043/Natural Science Foundation of Heilongjiang Province/
PT  - Journal Article
DEP - 20210128
PL  - Germany
TA  - Mol Genet Genomics
JT  - Molecular genetics and genomics : MGG
JID - 101093320
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Heterogeneous Nuclear Ribonucleoprotein A1)
RN  - 0 (MIRN326 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (XIST non-coding RNA)
RN  - 0 (hnRNPA1 protein, human)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - Databases, Genetic
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - Genetic Predisposition to Disease
MH  - Heterogeneous Nuclear Ribonucleoprotein A1/*genetics
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Molecular Sequence Annotation
MH  - Multiple Sclerosis/*genetics
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - CeRNAs
OT  - LncRNAs
OT  - MS
OT  - ceRNA network
OT  - lncRNA-mediated competing triplets
EDAT- 2021/01/29 06:00
MHDA- 2021/02/26 06:00
CRDT- 2021/01/28 12:14
PHST- 2020/06/26 00:00 [received]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2021/01/29 06:00 [pubmed]
PHST- 2021/02/26 06:00 [medline]
PHST- 2021/01/28 12:14 [entrez]
AID - 10.1007/s00438-020-01750-1 [pii]
AID - 10.1007/s00438-020-01750-1 [doi]
PST - ppublish
SO  - Mol Genet Genomics. 2021 Mar;296(2):423-435. doi: 10.1007/s00438-020-01750-1. 
      Epub 2021 Jan 28.