PMID- 33512500
OWN - NLM
STAT- MEDLINE
DCOM- 20220323
LR  - 20220330
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 46
IP  - 3
DP  - 2022 Mar 21
TI  - Development and Validation of an Analytical Method for Quantitation of Bisphenol 
      S in Rodent Plasma, Amniotic Fluid and Fetuses by UPLC-MS-MS.
PG  - 277-284
LID - 10.1093/jat/bkab008 [doi]
AB  - Bisphenol S (BPS) has been detected in personal care products, water, food and 
      indoor house dust, demonstrating the potential for human exposure. Due to limited 
      data to characterize the hazard of BPS, the National Toxicology Program (NTP) is 
      investigating the toxicity of BPS in rodent models. Generating systemic exposure 
      data is integral to putting toxicological findings into context. The objective of 
      this work was to develop and validate a method to quantitate free (unconjugated 
      parent) and total (free and all conjugated forms of) BPS in rodent plasma, 
      amniotic fluid and fetal homogenate in support of NTP studies. The method used 
      incubation with (total BPS) and without (free BPS) deconjugating enzyme and then 
      protein precipitation followed by ultra-performance liquid chromatography-tandem 
      mass spectrometry. In Sprague Dawley rat plasma, the method was linear (r >/= 0.99) 
      over the range 5-1,000 ng/mL, accurate (mean relative error (RE) </= +/-10.5%) and 
      precise (relative standard deviation (RSD) </= 7.7%). Mean recoveries were >/=93.1% 
      for both free and total analyses. The limits of detection were 1.15 ng/mL (free) 
      and 0.862 ng/mL (total) in plasma. The method was evaluated in the following 
      study matrices: (i) male Hsd:Sprague Dawley(R)SD(R) (HSD) rat plasma, (ii) female HSD 
      rat plasma, (iii) male B6C3F1 mouse plasma, (iv) female B6C3F1 mouse plasma, (v) 
      HSD rat gestational day (GD) 18 dam plasma, (vi) HSD rat GD 18 amniotic fluid, 
      (vii) HSD rat GD 18 fetal homogenate and (viii) HSD rat postnatal day 4 pup 
      plasma (mean %RE </= +/-8.2 and %RSD </= 8.7). Stability of BPS in extracted samples 
      was demonstrated for up to 7 days at various temperatures, and freeze-thaw 
      stability was demonstrated after three cycles over 7 days. BPS in various 
      matrices stored at -80 degrees C for at least 60 days was within 92.1-115% of Day 0 
      concentrations, demonstrating its stability in these matrices. These data 
      demonstrate that this simple method is suitable for determination of free and 
      total BPS in plasma, amniotic fluid and fetuses following exposure of rodents to 
      BPS.
CI  - (c) The Author(s) 2021. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Rehder Silinski, Melanie A
AU  - Rehder Silinski MA
AUID- ORCID: 0000-0003-0234-3798
AD  - Discovery Sciences Unit, RTI International, P.O. Box 12194, Research Triangle 
      Park, NC 27709, USA.
FAU - Fletcher, Brenda L
AU  - Fletcher BL
AD  - Discovery Sciences Unit, RTI International, P.O. Box 12194, Research Triangle 
      Park, NC 27709, USA.
FAU - Fernando, Reshan A
AU  - Fernando RA
AUID- ORCID: 0000-0002-9245-7790
AD  - Discovery Sciences Unit, RTI International, P.O. Box 12194, Research Triangle 
      Park, NC 27709, USA.
FAU - Robinson, Veronica G
AU  - Robinson VG
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
FAU - Waidyanatha, Suramya
AU  - Waidyanatha S
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
LA  - eng
GR  - HHSN273201400022C/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Phenols)
RN  - 0 (Sulfones)
RN  - 80-09-1 (bis(4-hydroxyphenyl)sulfone)
SB  - IM
MH  - *Amniotic Fluid
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid/methods
MH  - Female
MH  - Male
MH  - Mice
MH  - Phenols
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - Rodentia
MH  - Sulfones
MH  - *Tandem Mass Spectrometry/methods
PMC - PMC8935988
EDAT- 2021/01/30 06:00
MHDA- 2022/03/24 06:00
PMCR- 2022/01/09
CRDT- 2021/01/29 12:13
PHST- 2020/07/22 00:00 [received]
PHST- 2020/12/15 00:00 [revised]
PHST- 2021/01/08 00:00 [accepted]
PHST- 2021/01/30 06:00 [pubmed]
PHST- 2022/03/24 06:00 [medline]
PHST- 2021/01/29 12:13 [entrez]
PHST- 2022/01/09 00:00 [pmc-release]
AID - 6072573 [pii]
AID - bkab008 [pii]
AID - 10.1093/jat/bkab008 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2022 Mar 21;46(3):277-284. doi: 10.1093/jat/bkab008.