PMID- 33513930
OWN - NLM
STAT- MEDLINE
DCOM- 20210415
LR  - 20210415
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 3
DP  - 2021 Jan 27
TI  - Acer tataricum subsp. ginnala Inhibits Skin Photoaging via Regulating MAPK/AP-1, 
      NF-kappaB, and TGFbeta/Smad Signaling in UVB-Irradiated Human Dermal Fibroblasts.
LID - 10.3390/molecules26030662 [doi]
LID - 662
AB  - Skin, the organ protecting the human body from external factors, maintains 
      structural and tensile strength by containing many collagen fibrils, particularly 
      type I procollagen. However, oxidative stress by ultraviolet (UV) exposure causes 
      skin photoaging by activating collagen degradation and inhibiting collagen 
      synthesis. Acer tataricum subsp. ginnala extract (AGE) is a herbal medicine with 
      anti-inflammatory and anti-oxidative effects, but there is no report on the 
      protective effect against skin photoaging. Therefore, we conducted research 
      concentrating on the anti-photoaging effect of Acer tataricum subsp. ginnala (AG) 
      in UVB (20 mJ/cm(2))-irradiated human dermal fibroblasts (HDF). Then, various 
      concentrations (7.5, 15, 30 microg/mL) of AGE were treated in HDF for 24 h following 
      UVB irradiation. After we performed AGE treatment, the matrix metalloproteinase1 
      (MMP1) expression was downregulated, and the type I procollagen level was 
      recovered. Then, we investigated the mitogen-activated protein kinases/activator 
      protein 1 (MAPK/AP-1) and nuclear factor-kappaB (NF-kappaB) pathway, which induce 
      collagen breakdown by promoting the MMP1 level and pro-inflammatory cytokines. 
      The results indicated that AGE downregulates the expression of the MAPK/AP-1 
      pathway, leading to MMP1 reduction. AGE inhibits nuclear translocation of NF-kappaB 
      and inhibitor of nuclear factor-kappaB (IkappaB) degradation. Therefore, it downregulates 
      the expression of MMP1 and pro-inflammatory cytokines such as TNF-alpha, IL-1beta, and 
      IL-6 increased by UVB. Besides, the TGFbeta/Smad pathway, which is mainly 
      responsible for the collagen synthesis in the skin, was also analyzed. AGE 
      decreases the expression of Smad7 and increases TGFbetaRII expression and Smad3 
      phosphorylation. This means that AGE stimulates the TGFbeta/Smad pathway that plays 
      a critical role in promoting collagen synthesis. Thus, this study suggests that 
      AGE can be a functional material with anti-photoaging properties.
FAU - Jin, Yu-Jung
AU  - Jin YJ
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, Seoul 02447, Korea.
FAU - Ji, Yura
AU  - Ji Y
AUID- ORCID: 0000-0002-9074-5238
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, Seoul 02447, Korea.
FAU - Jang, Young-Pyo
AU  - Jang YP
AUID- ORCID: 0000-0001-5865-9228
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, Seoul 02447, Korea.
AD  - Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee 
      University, Seoul 02447, Korea.
FAU - Choung, Se-Young
AU  - Choung SY
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, Seoul 02447, Korea.
AD  - Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee 
      University, Seoul 02447, Korea.
LA  - eng
PT  - Journal Article
DEP - 20210127
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Collagen Type I)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (Smad Proteins)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Acer/*chemistry
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - Down-Regulation/drug effects
MH  - Fibroblasts/*drug effects/metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Signal Transduction/*drug effects
MH  - Skin/*drug effects/metabolism
MH  - Skin Aging/*drug effects
MH  - Smad Proteins/metabolism
MH  - Transcription Factor AP-1/metabolism
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC7865648
OTO - NOTNLM
OT  - Acer tataricum subsp. ginnala
OT  - MAPK/AP-1
OT  - NF-kB
OT  - TGFbeta/Smad
OT  - matrix metalloproteinase (MMP)
OT  - type I procollagen
COIS- There are no conflicts of interest to declare.
EDAT- 2021/01/31 06:00
MHDA- 2021/04/16 06:00
PMCR- 2021/01/27
CRDT- 2021/01/30 01:02
PHST- 2020/12/24 00:00 [received]
PHST- 2021/01/23 00:00 [revised]
PHST- 2021/01/23 00:00 [accepted]
PHST- 2021/01/30 01:02 [entrez]
PHST- 2021/01/31 06:00 [pubmed]
PHST- 2021/04/16 06:00 [medline]
PHST- 2021/01/27 00:00 [pmc-release]
AID - molecules26030662 [pii]
AID - molecules-26-00662 [pii]
AID - 10.3390/molecules26030662 [doi]
PST - epublish
SO  - Molecules. 2021 Jan 27;26(3):662. doi: 10.3390/molecules26030662.