PMID- 33516151
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210707
IS  - 1873-2682 (Electronic)
IS  - 1011-1344 (Linking)
VI  - 216
DP  - 2021 Mar
TI  - Characterization and photoprotective potentiality of lime dwelling Pseudomonas 
      mediated melanin as sunscreen agent against UV-B radiations.
PG  - 112126
LID - S1011-1344(21)00004-X [pii]
LID - 10.1016/j.jphotobiol.2021.112126 [doi]
AB  - Prolonged exposure to Ultra Violet Radiation (UVR) adversely alters the functions 
      of many skin cell types causing skin cancer and photoaging, which had led to 
      increase in demand for more safe and natural sunscreens against UVR. The present 
      study focuses on production, structural characterization and evaluation of 
      photoprotective nature of melanin pigment derived from lime dwelling Pseudomonas 
      sp. Melanin was characterized by solubility, UV-Vis, FT-IR, (13)C-CPMAS, ESI-MS 
      spectroscopy, including particle size, melting point and elemental analyses. In 
      vitro cytotoxicity and photo-protective effect of Pseudomonas derived melanin 
      (Mel-P) against UV-B (Broad Band-BB) radiations were assessed on mouse 
      fibroblasts NIH 3 T3 cell lines. Reactive Oxygen Species (ROS) generated in NIH 
      3 T3 cells upon UV-B (BB) exposure was determined and quantified by Fluorescent 
      microscopic and Flow cytometric analyses. A natural melanin obtained from 
      Pseudomonas sp. contains 5,6- dihydroxy indole 2-carboxyic acid (DHICA) as its 
      basic constituent and possess typical properties of eumelanin as revealed by the 
      characterization studies. Mel-P has shown cell viability of 61.33 +/- 6.58% at the 
      concentration of 500 mug/mL proving its non-cytotoxic effect. Owing to its 
      anti-oxidant property, melanin efficiently protected the mouse fibroblast cells 
      from UV-B (BB) irradiation in a dose dependant manner demonstrating its potential 
      as an active photoprotective agent.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Seelam, Siva Deepthi
AU  - Seelam SD
AD  - Department of Microbiology, Gulbarga University, Kalaburgi 585106, Karnataka, 
      India; Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of 
      Chemical Technology, Hyderabad 500007, Telangana, India. Electronic address: 
      deepoo216@gmail.com.
FAU - Agsar, Dayanand
AU  - Agsar D
AD  - Department of Microbiology, Gulbarga University, Kalaburgi 585106, Karnataka, 
      India. Electronic address: dayanandagsar@gmail.com.
FAU - Halmuthur M, Sampath Kumar
AU  - Halmuthur M SK
AD  - Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of 
      Chemical Technology, Hyderabad 500007, Telangana, India. Electronic address: 
      sampath@iict.res.in.
FAU - Reddy Shetty, Prakasham
AU  - Reddy Shetty P
AD  - Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of 
      Chemical Technology, Hyderabad 500007, Telangana, India. Electronic address: 
      prakasham@iict.res.in.
FAU - Vemireddy, Sravanthi
AU  - Vemireddy S
AD  - Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of 
      Chemical Technology, Hyderabad 500007, Telangana, India. Electronic address: 
      vemireddy.sravanthireddy@gmail.com.
FAU - Reddy, K Mohan
AU  - Reddy KM
AD  - Department of Biotechnology, Gulbarga University, Kalaburgi 585106, Karnataka, 
      India. Electronic address: kmrphdgug@mail.com.
FAU - Umesh, M K
AU  - Umesh MK
AD  - Department of Microbiology, Gulbarga University, Kalaburgi 585106, Karnataka, 
      India. Electronic address: umeshbt720@gmail.com.
FAU - Rajitha, C H
AU  - Rajitha CH
AD  - Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of 
      Chemical Technology, Hyderabad 500007, Telangana, India. Electronic address: 
      raajiguduru@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210120
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Antioxidants)
RN  - 0 (Calcium Compounds)
RN  - 0 (Melanins)
RN  - 0 (Oxides)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sunscreening Agents)
RN  - 12627-86-0 (eumelanin)
RN  - C7X2M0VVNH (lime)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antioxidants/chemistry/metabolism
MH  - Calcium Compounds/*chemistry/metabolism
MH  - Fibroblasts/cytology/radiation effects
MH  - Humans
MH  - Melanins/*chemistry/metabolism/pharmacology
MH  - Mice
MH  - Oxides/*chemistry/metabolism
MH  - Protective Agents/*chemistry/pharmacology
MH  - Pseudomonas/metabolism/*radiation effects
MH  - Reactive Oxygen Species/chemistry/metabolism
MH  - Skin
MH  - Skin Neoplasms/*prevention & control
MH  - Solubility
MH  - Sunscreening Agents/*chemistry/metabolism
MH  - Ultraviolet Rays
OTO - NOTNLM
OT  - Melanin
OT  - Natural pigment
OT  - Non-cytotoxic
OT  - ROS scavenging
OT  - UV-B protectant
EDAT- 2021/01/31 06:00
MHDA- 2021/07/08 06:00
CRDT- 2021/01/30 20:17
PHST- 2019/12/27 00:00 [received]
PHST- 2020/12/13 00:00 [revised]
PHST- 2021/01/14 00:00 [accepted]
PHST- 2021/01/31 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2021/01/30 20:17 [entrez]
AID - S1011-1344(21)00004-X [pii]
AID - 10.1016/j.jphotobiol.2021.112126 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2021 Mar;216:112126. doi: 
      10.1016/j.jphotobiol.2021.112126. Epub 2021 Jan 20.