PMID- 33517147
OWN - NLM
STAT- MEDLINE
DCOM- 20210625
LR  - 20210625
IS  - 1879-0429 (Electronic)
IS  - 0958-1669 (Linking)
VI  - 68
DP  - 2021 Apr
TI  - Dendritic cell metabolism: moving beyond in vitro-culture-generated paradigms.
PG  - 202-212
LID - S0958-1669(20)30195-6 [pii]
LID - 10.1016/j.copbio.2020.12.010 [doi]
AB  - Dendritic cells (DCs) are key orchestrators of immunity and tolerance. It has 
      become evident that DC function can be influenced by cellular metabolic programs. 
      However, conclusions from early metabolic studies using in vitro GM-CSF DC 
      cultures fail to correlate with bona fide DC populations. Here, we discuss the 
      existing paradigms in the DC metabolism field, focusing on the limitations of the 
      models utilized. Furthermore, we introduce alternative models to generate DCs in 
      vitro that better emulate DCs found in vivo. Finally, we highlight new techniques 
      to evaluate DC metabolism at the single-cell level. The combination of these two 
      strategies could help advance the DC metabolism field towards a more 
      physiological understanding, which is crucial for the development of effective 
      DC-based therapies.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Minarrieta, Lucia
AU  - Minarrieta L
AD  - Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and 
      Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, 
      Canada.
FAU - Velasquez, Lis Noelia
AU  - Velasquez LN
AD  - Institute of Medical Microbiology and Hygiene, University Medical Centre of the 
      Johannes Gutenberg-University Mainz, Mainz, Germany.
FAU - Sparwasser, Tim
AU  - Sparwasser T
AD  - Institute of Medical Microbiology and Hygiene, University Medical Centre of the 
      Johannes Gutenberg-University Mainz, Mainz, Germany.
FAU - Berod, Luciana
AU  - Berod L
AD  - Institute of Molecular Medicine, University Medical Centre of the Johannes 
      Gutenberg-University Mainz, Mainz, Germany. Electronic address: 
      berod@uni-mainz.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20210128
PL  - England
TA  - Curr Opin Biotechnol
JT  - Current opinion in biotechnology
JID - 9100492
SB  - IM
MH  - Cell Differentiation
MH  - *Dendritic Cells
EDAT- 2021/02/01 06:00
MHDA- 2021/06/29 06:00
CRDT- 2021/01/31 20:32
PHST- 2020/09/09 00:00 [received]
PHST- 2020/11/25 00:00 [revised]
PHST- 2020/12/14 00:00 [accepted]
PHST- 2021/02/01 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2021/01/31 20:32 [entrez]
AID - S0958-1669(20)30195-6 [pii]
AID - 10.1016/j.copbio.2020.12.010 [doi]
PST - ppublish
SO  - Curr Opin Biotechnol. 2021 Apr;68:202-212. doi: 10.1016/j.copbio.2020.12.010. 
      Epub 2021 Jan 28.