PMID- 33517163 OWN - NLM STAT- MEDLINE DCOM- 20210512 LR - 20210512 IS - 1532-2661 (Electronic) IS - 0034-5288 (Linking) VI - 135 DP - 2021 Mar TI - A chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine is safe under international guidelines and effective both in experimental and field conditions. PG - 143-152 LID - S0034-5288(21)00024-2 [pii] LID - 10.1016/j.rvsc.2021.01.012 [doi] AB - Vaccination is currently the most effective strategy to control porcine reproductive and respiratory syndrome (PRRS). New-generation PRRS vaccines are required to be safe and broadly cross-protective. We have recently created the chimeric PRRS virus K418DM which proved to be a good vaccine candidate under field conditions. In the present study, we designed safety and efficacy tests under experimental and field conditions for further evaluation of K418DM1.1, a plaque-purified K418DM. In the homologous challenge study, K418DM1.1 induced high serum virus neutralization (SVN) antibody titers (i.e., 4.2 log(2) +/- 1.7) at 21 days post-challenge (dpc) and provided protection as demonstrated by the significantly lower levels of viremia at 3 and 7 dpc and significantly lower microscopic lung lesion scores compared to the unvaccinated group. K418DM1.1 was also protective in the heterologous challenge study, with vaccinated pigs showing significantly lower levels of viremia at 14 dpc compared to the unvaccinated pigs. A field study was performed to evaluate the efficacy of K418DM1.1 against heterologous exposure and vaccinated pigs presented significantly lower viremia than unvaccinated pigs. According to the safety test for the examination of virulence reversion, no infectivity was observed in tissue homogenate filtrate both in the vaccinated and comingled groups. Thus, the risk of virulence, as well as transmission, appeared negligible. These overall results indicate that K418DM1.1 is a good vaccine candidate based on its safety and protective efficacy. CI - Copyright (c) 2021 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Choi, Hwi-Yeon AU - Choi HY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, So-Hyun AU - Lee SH AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Ahn, So-Hyeun AU - Ahn SH AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Choi, Jong-Chul AU - Choi JC AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Jeong, Ji-Yun AU - Jeong JY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, Beom-Joo AU - Lee BJ AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Kang, Yeong-Lim AU - Kang YL AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Hwang, Seong-Soo AU - Hwang SS AD - Samhwa Breedings Agri. Inc., 435, Sinjin-ri, Gwangcheon-eup, Hongseong-gun, Chungcheongnam-Do 350-900, Republic of Korea. FAU - Lee, Jung-Keun AU - Lee JK AD - Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, 19555, North 59th Avenue, Glendale, AZ 85308, USA. FAU - Lee, Sang-Won AU - Lee SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Park, Seung-Yong AU - Park SY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Song, Chang-Seon AU - Song CS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Choi, In-Soo AU - Choi IS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, Joong-Bok AU - Lee JB AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: virus@konkuk.ac.kr. LA - eng PT - Journal Article DEP - 20210123 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Porcine Reproductive and Respiratory Syndrome/*immunology MH - Porcine respiratory and reproductive syndrome virus/*immunology MH - Sus scrofa MH - Swine MH - Vaccination/*veterinary MH - Viral Vaccines/*adverse effects MH - Viremia/immunology/*veterinary OTO - NOTNLM OT - Chimera OT - Efficacy OT - K418DM OT - PRRSV OT - Safety OT - Vaccine EDAT- 2021/02/01 06:00 MHDA- 2021/05/13 06:00 CRDT- 2021/01/31 20:33 PHST- 2020/08/10 00:00 [received] PHST- 2021/01/11 00:00 [revised] PHST- 2021/01/21 00:00 [accepted] PHST- 2021/02/01 06:00 [pubmed] PHST- 2021/05/13 06:00 [medline] PHST- 2021/01/31 20:33 [entrez] AID - S0034-5288(21)00024-2 [pii] AID - 10.1016/j.rvsc.2021.01.012 [doi] PST - ppublish SO - Res Vet Sci. 2021 Mar;135:143-152. doi: 10.1016/j.rvsc.2021.01.012. Epub 2021 Jan 23.