PMID- 33517972
OWN - NLM
STAT- MEDLINE
DCOM- 20210202
LR  - 20210202
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 50
IP  - 1
DP  - 2021 Jan
TI  - [Determination of domoic acid in shellfish by liquid chromatography- tandem mass 
      spectrometry coupled with dispersive solid phase extraction].
PG  - 116-142
LID - 10.19813/j.cnki.weishengyanjiu.2021.01.020 [doi]
AB  - OBJECTIVE: A method utilizing liquid chromatography-tandem mass 
      spectrometry(LC-MS/MS) coupled with dispersive solid phase extraction for 
      quantitative analysis of domoic acid in four kinds of shellfish was established. 
      METHODS: The sample of 0. 1 g was extracted with 25% methanol aqueous solution, 
      the extract was purified by dispersive solid phase extraction with 50 mg HLB and 
      5 mg GCB, and then filtered through a PTFE membrane. The analytes were separated 
      on a C_(18) column(100 mmx2. 1 mm, 1. 9 mum), and detected in selected reaction 
      monitoring(SRM) mode via positive electrospray ionization. The matrix matching 
      and external standard method was used for quantitation. RESULTS: Domoic acid 
      showed good linearity in the concentration range between 1. 0 ng/mL and 50. 0 
      ng/mL with correlation coefficients higher than 0. 9994. The detection limits of 
      domoic acid in shellfish was 5 mug/kg. The inter-and intra-day recoveries were 91. 
      6%-109. 2% and 90. 9%-109. 3%, respectively. The inter-and intra-day ralitive 
      standard deviations(RSDs) were lower than 8. 2% at spiked concentrations of 20, 
      50 and 100 mug/kg. CONCLUSION: The method is accurate, fast, easy to operate, 
      which can satisfy the requirements of public health emergency testing or routine 
      testing.
FAU - Fang, Li
AU  - Fang L
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 
      Municipal Center for Disease Control and Prevention, Zhoushan 316021, China.
FAU - Wu, Fan
AU  - Wu F
AD  - Wannan Medical College, School of Laboratory Medicine, Wuhu 241002, China.
FAU - Qiu, Fengmei
AU  - Qiu F
AD  - Daishan Center for Disease Control and Prevention, Zhoushan 316200, China.
FAU - Yu, Xinwei
AU  - Yu X
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 
      Municipal Center for Disease Control and Prevention, Zhoushan 316021, China.
FAU - Zhu, Yanhua
AU  - Zhu Y
AD  - School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - M02525818H (domoic acid)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Kainic Acid/analogs & derivatives
MH  - Shellfish/analysis
MH  - *Solid Phase Extraction
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - amnesic shellfish poisoning
OT  - dispersive solid phase extraction
OT  - domoic acid
OT  - liquid chromatography-tandem mass spectrometry
OT  - shellfish
EDAT- 2021/02/02 06:00
MHDA- 2021/02/03 06:00
CRDT- 2021/02/01 05:30
PHST- 2021/02/01 05:30 [entrez]
PHST- 2021/02/02 06:00 [pubmed]
PHST- 2021/02/03 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2021.01.020 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2021 Jan;50(1):116-142. doi: 
      10.19813/j.cnki.weishengyanjiu.2021.01.020.