PMID- 33518676
OWN - NLM
STAT- MEDLINE
DCOM- 20210927
LR  - 20210927
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 44
IP  - 2
DP  - 2021
TI  - Anti-allergic Inflammatory Components from the Leaves of Piper crocatum Ruiz & 
      Pav.
PG  - 245-250
LID - 10.1248/bpb.b20-00726 [doi]
AB  - Piper crocatum Ruiz & Pav. (P. crocatum), a traditional medicinal plant, has been 
      shown to possess various pharmacological activities, including anticancer 
      activity, antioxidant activity, antibacterial activity, anti-hyperglycemic 
      activity, anti-allergic inflammatory activity and others. To identify the 
      potential anti-allergic inflammatory effective constituents of P. crocatum, 13 
      single compounds were isolated from the methanol extract of P. crocatum leaves, 
      and their structures were identified by contrasting their NMR spectroscopic data 
      and previously published papers. First, the anti-allergic inflammatory activities 
      of these single compounds were examined by accessing immune function related 
      biomarkers such as nitric oxide (NO) and beta-hexosaminidase. We found that the 
      methanol extract and catechaldehyde (compound 1) potently suppressed NO 
      production. Additionally, Western blot analysis showed that P. crocatum methanol 
      extract and compound 1 suppressed the production of NO by reducing inducible 
      nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-induced 
      RAW264.7 macrophages. Consistent with these observations, P. crocatum methanol 
      extract and compound 1 remarkably decreased beta-hexosaminidase release from RBL-2H3 
      cells stimulated with 2,4-dinitrophenylated bovine serum albumin 
      (DNP-BSA)-specific immunoglobulin E (IgE) antibodies. Furthermore, 
      3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 
      (MTS) assay indicated that P. crocatum methanol extract and compound 1 exhibited 
      no cytotoxicity to RAW264.7 and RBL-2H3 cells. Based on these findings, compound 
      1 is suggested as an active anti-allergic inflammatory component of P. crocatum.
FAU - Gong, Yue
AU  - Gong Y
AD  - College of Pharmacy and Research Institute of Drug Development, Chonnam National 
      University.
FAU - Li, Hong Xu
AU  - Li HX
AD  - College of Pharmacy, Chungnam National University.
FAU - Guo, Rui Hong
AU  - Guo RH
AD  - College of Pharmacy and Research Institute of Drug Development, Chonnam National 
      University.
FAU - Widowati, Wahyu
AU  - Widowati W
AD  - Medical Research Centre, Faculty of Medicine, Maranatha Christian University.
FAU - Kim, Young Ho
AU  - Kim YH
AD  - College of Pharmacy, Chungnam National University.
FAU - Yang, Seo Young
AU  - Yang SY
AD  - College of Pharmacy, Chungnam National University.
FAU - Kim, Young Ran
AU  - Kim YR
AD  - College of Pharmacy and Research Institute of Drug Development, Chonnam National 
      University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/isolation & purification/*pharmacology
MH  - Anti-Inflammatory Agents/isolation & purification/*pharmacology
MH  - Cell Line, Tumor
MH  - Drug Evaluation, Preclinical
MH  - Methanol/chemistry
MH  - Mice
MH  - Piper/*chemistry
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Plant Leaves/chemistry
MH  - RAW 264.7 Cells
MH  - Rats
OTO - NOTNLM
OT  - Piper crocatum Ruiz & Pav.
OT  - allergic inflammation
OT  - catechaldehyde
OT  - inducible nitric oxide synthase
OT  - nitric oxide
OT  - beta-hexosaminidase
EDAT- 2021/02/02 06:00
MHDA- 2021/09/28 06:00
CRDT- 2021/02/01 05:50
PHST- 2021/02/01 05:50 [entrez]
PHST- 2021/02/02 06:00 [pubmed]
PHST- 2021/09/28 06:00 [medline]
AID - 10.1248/bpb.b20-00726 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2021;44(2):245-250. doi: 10.1248/bpb.b20-00726.