PMID- 33522207
OWN - NLM
STAT- MEDLINE
DCOM- 20210907
LR  - 20210907
IS  - 2589-451X (Electronic)
IS  - 0255-2922 (Linking)
VI  - 41
IP  - 1
DP  - 2021 Feb
TI  - Relationship between cardiotonic activity of Fuzi (Radix Aconiti Lateralis 
      Preparata) and its fingerprint determined by liquid chromatography-mass 
      spectrometry.
PG  - 140-149
LID - 10.19852/j.cnki.jtcm.2021.01.016 [doi]
AB  - OBJECTIVE: To investigate the relationship between the cardiotonic activity of 
      Fuzi (Radix Aconiti Lateralis Preparata, RALP) and its fingerprint determined by 
      liquid chromatography-mass spectrometry (LC-MS). METHODS: First, the fingerprints 
      of six processed products of RALP were established by high performance liquid 
      chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) 
      followed by analysis of the principal component of the relative peak area of its 
      common peaks. Next, the scores of the first five principal components were used 
      as input for an artificial neural network (ANN). Additionally, the therapeutic 
      effect of RALP was assessed by measuring the hemodynamic indexes of heart failure 
      model rats. Subsequently, fluorescence semi-quantitative polymerase chain 
      reaction and an enzyme-linked immunosorbent assay kit were used to determine the 
      effects of RALP-processed products on the serum levels of noradrenaline (NA), 
      angiotensin-Ⅰ (Ang-Ⅰ), and the expression of beta-norepinephrine receptor mRNA 
      (beta-NRm) in the rat cardiac tissues. P < 0.05 was used as the output of the ANN. 
      Finally, a network was constructed to display the relationship between the LC-MS 
      fingerprints and the cardiotonic activity of the RALP-processed products. 
      RESULTS: Several types of RALPs can improve diastolic function, systolic function 
      and heart rate. On the basis of the findings from the principal component 
      analysis (PCA) of 16 common peaks of fingerprints of six RALP-processed products, 
      it was revealed that the first five principal components may include 100% of the 
      information of the original data. As observed from the multilayer perceptron 
      neural network analysis, principal component 4 presented with the strongest 
      effects on serum levels of NA and Ang-Ⅰ in rats, while principal component 1 
      exerted the greatest effect on beta-NRm expression in cardiac tissue. CONCLUSION: 
      The key findings obtained from this study indicated that the network constructed 
      by the PCA-ANN may predict pharmacodynamic effects of the main ingredients of 
      Traditional Chinese Medicine (TCM). This method may serve as a new approach to 
      identify the relationship between LC-MS fingerprints and the pharmacodynamic 
      effects of TCM ingredients.
FAU - Tong, Hengli
AU  - Tong H
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 
      330004, China.
FAU - Zhu, Jing
AU  - Zhu J
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 
      330004, China.
FAU - Gong, Feipeng
AU  - Gong F
AD  - Department of Orthopaedic, Jiangxi Provincial People's Hospital, Nanchang 330006, 
      China.
FAU - Zhong, Lingyun
AU  - Zhong L
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 
      330004, China.
FAU - Xu, Ting
AU  - Xu T
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 
      330004, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
RN  - 0 (Cardiotonic Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Receptors, Adrenergic)
RN  - 11002-13-4 (Angiotensinogen)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Aconitum/*chemistry
MH  - Angiotensinogen/genetics/metabolism
MH  - Animals
MH  - Cardiotonic Agents/administration & dosage/*chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Drugs, Chinese Herbal/administration & dosage/*chemistry
MH  - Heart Failure/*drug therapy/genetics/metabolism
MH  - Humans
MH  - Male
MH  - Mass Spectrometry
MH  - Norepinephrine/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic/genetics/metabolism
OTO - NOTNLM
OT  - Artificial neural network
OT  - Chromatography, high pressure liquid
OT  - Ginger
OT  - Mass spectrometry
OT  - Principal component analysi
OT  - Radix Aconiti Lateralis Preparata
EDAT- 2021/02/02 06:00
MHDA- 2021/09/08 06:00
CRDT- 2021/02/01 06:10
PHST- 2021/02/01 06:10 [entrez]
PHST- 2021/02/02 06:00 [pubmed]
PHST- 2021/09/08 06:00 [medline]
AID - 3131 [pii]
AID - 10.19852/j.cnki.jtcm.2021.01.016 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2021 Feb;41(1):140-149. doi: 10.19852/j.cnki.jtcm.2021.01.016.